PDF(Pubmed)
Abstract：
Bacillus species appearas the most attractive plant growth-promoting rhizobacteria (PGPR) and alternative to synthetic chemical pesticides. The present study examined the antagonistic potential of spore forming-Bacilli isolated from organic farm soil samples of Allahabad, India. Eighty-seven Bacillus strains were isolated and characterized based on their morphological, plant growth promoting traits and molecular characteristics. The diversity analysis used 16S-rDNA, BOX-element, and enterobacterial repetitive intergenic consensus. Two strains, PR30 and PR32, later identified as Bacillus sp., exhibited potent in vitro antagonistic activity against Ralstonia solanaceorum. These isolates produced copious amounts of multiple PGP traits, such as indole-3-acetic acid (40.0 and 54.5 μg/mL), phosphate solubilization index (PSI) (4.4 and 5.3), ammonia, siderophore (3 and 4 cm), and 1-aminocyclopropane-1-carboxylate deaminase (8.1and 9.2 μM/mg//h) and hydrogen cyanide. These isolates were subjected to the antibiotic sensitivity test. The two potent isolates based on the higher antagonistic and the best plant growth-promoting ability were selected for plant growth-promoting response studies in tomatoe, broccoli, and chickpea. In the pot study, Bacillus subtilis (PR30 and PR31) showed significant improvement in seed germination (27-34%), root length (20-50%), shoot length (20-40%), vigor index (50-75%), carotenoid content (0.543-1.733), and lycopene content (2.333-2.646 mg/100 g) in tomato, broccoli, and chickpea. The present study demonstrated the production of multiple plant growth-promoting traits by the isolates and their potential as effective bioinoculants for plant growth promotion and biocontrol of phytopathogens.
摘要：
芽孢杆菌物种似乎是最有吸引力的促进植物生长的根际细菌（PGPR），也是合成化学农药的替代品。本研究检查了从阿拉哈巴德的有机农场土壤样品中分离出的孢子形成芽孢杆菌的拮抗潜力，印度。对87株芽孢杆菌属菌株进行了分离和鉴定,植物生长促进性状和分子特性。多样性分析使用16S-rDNA,BOX元素,和肠细菌重复基因间共识。两株，PR30和PR32，后来被鉴定为芽孢杆菌属。,对茄菌表现出有效的体外拮抗活性。这些分离株产生了大量的多种PGP性状，如吲哚-3-乙酸（40.0和54.5μg/mL），磷酸盐增溶指数（PSI）（4.4和5.3），氨,铁载体（3和4厘米），和1-氨基环丙烷-1-羧酸脱氨酶（8.1和9.2μM/mg//h）和氰化氢。对这些分离物进行抗生素敏感性试验。基于较高的拮抗作用和最佳的植物生长促进能力，选择了两个有效的分离株用于番茄的植物生长促进反应研究，西兰花,还有鹰嘴豆.在盆栽研究中，枯草芽孢杆菌(PR30和PR31)在种子萌发方面表现出显著的改善(27-34%),根长（20-50%），拍摄长度（20-40％），活力指数（50-75%），类胡萝卜素含量（0.543-1.733），番茄中的番茄红素含量（2.333-2.646mg/100g），西兰花,还有鹰嘴豆.本研究证明了分离株产生的多种植物生长促进性状及其作为植物生长促进和植物病原体生物防治的有效生物接种剂的潜力。
