筋膜病,一种全球重新出现的人畜共患疾病,主要是由肝片吸虫寄生虫感染引起的,通常被称为肝吸虫。这种疾病对牲畜生产力有相当大的影响。这项研究旨在评估山羊的fl虫负担和粪便卵数,这些山羊施用了组织蛋白酶L模拟表位的噬菌体克隆,然后感染了肝肝囊虫。此外,疫苗接种对生殖系统组织学的影响,特别是与成年寄生虫的卵生成有关,被检查过。总共二十四只山羊,在棚子里长大,分为四组,每组六只动物。这些组是随机分配的。然后对山羊进行两轮疫苗接种。每次疫苗接种涉及施用1×1013个含有组织蛋白酶L2特异性模拟表位的噬菌体颗粒(第1组:PPIRNGK),组织蛋白酶L1(第2组:DPWWLKQ),和组织蛋白酶L1(第3组:SGTFLFS)。在第0周和第4周进行免疫,并将QuilA佐剂与模拟表位组合使用。对照组给予磷酸盐缓冲盐水(PBS)(第4组)。在第6周时,所有组均口服感染200例肝胆。初次免疫后第22周,受试者被安乐死,从胆管和肝组织中取出成年肝肠球菌标本,随后量化。对标本进行了完整的组织学检查,以检查生殖系统,包括睾丸,子房,卵黄,Mehlis\'腺体,还有子宫.挑战后的平均侥幸负担减少了50.4%,62.2%,在接受含有组织蛋白酶L2PPIRNGK的疫苗接种的山羊中,有75.3%(p<0.05),组织蛋白酶L1DPWWLKQ,和组织蛋白酶L1SGTFLFS,分别。接受疫苗接种的动物表现出寄生虫卵产生的显著减少。接种山羊的IgG1和IgG2同种型水平明显高于对照组,表明保护与混合的Th1/Th2免疫应答的诱导有关。对山羊施用组织蛋白酶L在诱导肝吸虫生殖器官的组织学损伤方面表现出适度的功效,导致鸡蛋产量减少。
Fasciolosis, a globally re-emerging zoonotic disease, is mostly caused by the parasitic infection with Fasciola hepatica, often known as the liver fluke. This disease has a considerable impact on livestock productivity. This study aimed to evaluate the fluke burdens and faecal egg counts in goats that were administered phage clones of cathepsin L mimotopes and then infected with F. hepatica metacercariae. Additionally, the impact of vaccination on the histology of the reproductive system, specifically related to egg generation in adult parasites, was examined. A total of twenty-four goats, which were raised in sheds, were divided into four groups consisting of six animals each. These groups were randomly assigned. The goats were then subjected to two rounds of vaccination. Each vaccination involved the administration of 1 × 1013 phage particles containing specific mimotopes for cathepsin L2 (group 1: PPIRNGK), cathepsin L1 (group 2: DPWWLKQ), and cathepsin L1 (group 3: SGTFLFS). The immunisations were carried out on weeks 0 and 4, and the Quil A adjuvant was used in combination with the mimotopes. The control group was administered phosphate-buffered saline (PBS) (group 4). At week 6, all groups were orally infected with 200 metacercariae of F. hepatica. At week 22 following the initial immunisation, the subjects were euthanised, and adult F. hepatica specimens were retrieved from the bile ducts and liver tissue, and subsequently quantified. The specimens underwent whole-mount histology for the examination of the reproductive system, including the testis, ovary, vitellaria, Mehlis\' gland, and uterus. The mean fluke burdens following the challenge were seen to decrease by 50.4%, 62.2%, and 75.3% (p < 0.05) in goats that received vaccinations containing cathepsin L2 PPIRNGK, cathepsin L1 DPWWLKQ, and cathepsin L1 SGTFLFS, respectively. Animals that received vaccination exhibited a significant reduction in the production of parasite eggs. The levels of IgG1 and IgG2 isotypes in vaccinated goats were significantly higher than in the control group, indicating that protection is associated with the induction of a mixed Th1/Th2 immune response. The administration of cathepsin L to goats exhibits a modest level of efficacy in inducing histological impairment in the reproductive organs of liver flukes, resulting in a reduction in egg output.