背景:内生真菌(EF)存在于植物中,不会造成伤害,并提供诸如增强营养和产生生物活性化合物等益处,改善寄主植物的药用特性。选择具有既定药用特性的植物来研究EF很重要,因为它可以更深入地了解他们的影响。因此,本研究旨在探讨接种药用植物紫苏后EF的影响,特别关注它们在增强药用特性方面的作用。
结果:在当前的研究中,两个EF的影响,即,接种后,在紫苏叶片上观察到了从枝条中分离出的Irpexlenis和Schizhyllumcommunity。植物分为四组,即A组:对照组,B组:接种I.lenis;C组:接种S.commune;D组:同时接种EF。I.lenis的接种影响显示叶绿素a浓度增加(5.32mg/g),叶绿素b(4.46mg/g),总叶绿素含量(9.78mg/g),蛋白质(68.517±0.77mg/g),碳水化合物(137.886±13.71毫克/克),和粗纤维(3.333±0.37%)。此外,接种I.lenis的植物显示出最高的P浓度(14605mg/kg),Mg(4964.320mg/kg),Ca(27389.400mg/kg),和Mn(86.883mg/kg)。植物化学分析的结果还表明总黄酮含量增加(2.347mg/g),酚类(3.086mg/g),单宁(3.902毫克/克),和接种I.lenis的P.frutescens叶提取物中的生物碱(1.037mg/g)。因此,总的来说,在B组中观察到最好的接种结果,即接种I.lenis。甲醇叶提取物的GC-MS分析显示10种生物活性成分,包括9-十八烯酸(Z)-,甲酯,和十六烷酸,甲酯是所有组的主要成分。苯酚(没食子酸)和类黄酮(芦丁,山奈酚,和槲皮素)也通过HPTLC分析观察到接种后增加。与对照组相比,通过DPPH(抑制%:83.45µg/ml)和FRAP(2.980µMFe(II)当量)分析,植物化学物质含量的增加与抗氧化潜力的提高有关。
结论:用I.lenis接种显着增强了营养成分的吸收,植物化学物质,和抗氧化特性,表明它有可能提高寄主植物的治疗性能。
BACKGROUND: Endophytic fungi (EF) reside within plants without causing harm and provide benefits such as enhancing nutrients and producing bioactive compounds, which improve the medicinal properties of host plants. Selecting plants with established medicinal properties for studying EF is important, as it allows a deeper understanding of their influence. Therefore, the study aimed to investigate the impact of EF after inoculating the medicinal plant Perilla frutescens, specifically focusing on their role in enhancing medicinal properties.
RESULTS: In the current study, the impact of two EF i.e., Irpex lenis and Schizophyllum commune isolated from A. bracteosa was observed on plant Perilla frutescens leaves after inoculation. Plants were divided into four groups i.e., group A: the control group, group B: inoculated with I. lenis; group C: inoculated with S. commune and group D: inoculated with both the EF. Inoculation impact of I. lenis showed an increase in the concentration of chlorophyll a (5.32 mg/g), chlorophyll b (4.46 mg/g), total chlorophyll content (9.78 mg/g), protein (68.517 ± 0.77 mg/g), carbohydrates (137.886 ± 13.71 mg/g), and crude fiber (3.333 ± 0.37%). Furthermore, the plants inoculated with I. lenis showed the highest concentrations of P (14605 mg/kg), Mg (4964.320 mg/kg), Ca (27389.400 mg/kg), and Mn (86.883 mg/kg). The results of the phytochemical analysis also indicated an increased content of total flavonoids (2.347 mg/g), phenols (3.086 mg/g), tannins (3.902 mg/g), and alkaloids (1.037 mg/g) in the leaf extract of P. frutescens inoculated with I. lenis. Thus, overall the best results of inoculation were observed in Group B i.e. inoculated with I. lenis. GC-MS analysis of methanol leaf extract showed ten bioactive constituents, including 9-Octadecenoic acid (Z)-, methyl ester, and hexadecanoic acid, methyl ester as major constituents found in all the groups of P. frutescens leaves. The phenol (gallic acid) and flavonoids (rutin, kaempferol, and quercetin) were also observed to increase after inoculation by HPTLC analysis. The enhancement in the phytochemical content was co-related with improved anti-oxidant potential which was analyzed by DPPH (% Inhibition: 83.45 µg/ml) and FRAP (2.980 µM Fe (II) equivalent) assay as compared with the control group.
CONCLUSIONS: Inoculation with I. lenis significantly enhances the uptake of nutritional constituents, phytochemicals, and antioxidant properties in P. frutescens, suggesting its potential to boost the therapeutic properties of host plants.