PDF(Pubmed)
Abstract：
An excessive inflammatory response of the gastrointestinal tract is recognized as one of the major contributors to ulcerative colitis (UC). Despite this, effective preventive approaches for UC remain limited. Rosmarinic acid (RA), an enriched fraction from Perilla frutescens, has been shown to exert beneficial effects on disease-related inflammatory disorders. However, RA-enriched perilla seed meal (RAPSM) and perilla seed (RAPS) extracts have not been investigated in dextran sulfate sodium (DSS)-induced UC in mice. RAPSM and RAPS were extracted using the solvent-partitioning method and analyzed with high-pressure liquid chromatography (HPLC). Mice with UC induced using 2.5% DSS for 7 days were pretreated with RAPSM and RAPS (50, 250, 500 mg/kg). Then, the clinical manifestation, colonic histopathology, and serum proinflammatory cytokines were determined. Indeed, DSS-induced UC mice exhibited colonic pathological defects including an impaired colon structure, colon length shortening, and increased serum proinflammatory cytokines. However, RAPSM and RAPS had a protective effect at all doses by attenuating colonic pathology in DSS-induced UC mice, potentially through the suppression of proinflammatory cytokines. Concentrations of 50 mg/kg of RAPSM and RAPS were sufficient to achieve a beneficial effect in UC mice. This suggests that RAPSM and RAPS have a preventive effect against DSS-induced UC, potentially through alleviating inflammatory responses and relieving severe inflammation in the colon.
摘要：
胃肠道的过度炎症反应被认为是溃疡性结肠炎(UC)的主要原因之一。尽管如此,UC的有效预防方法仍然有限。迷迭香酸(RA),来自紫苏的富集部分，已被证明对疾病相关的炎性疾病发挥有益作用。然而,在葡聚糖硫酸钠（DSS）诱导的小鼠UC中尚未研究富含RA的紫苏种子粉（RAPSM）和紫苏种子（RAPS）提取物。使用溶剂分配方法提取RAPSM和RAPS，并用高压液相色谱（HPLC）进行分析。用RAPSM和RAPS(50、250、500mg/kg)预处理使用2.5%DSS诱导的UC小鼠7天。然后，临床表现，结肠组织病理学,并测定血清促炎细胞因子。的确,DSS诱导的UC小鼠表现出结肠病理缺陷，包括受损的结肠结构，结肠长度缩短，血清促炎细胞因子增加。然而,在DSS诱导的UC小鼠中，RAPSM和RAPS在所有剂量下都有保护作用，可能通过抑制促炎细胞因子。50mg/kgRAPSM和RAPS的浓度足以在UC小鼠中实现有益效果。这表明RAPSM和RAPS对DSS引起的UC有预防作用，可能通过减轻炎症反应和缓解结肠中的严重炎症。
