■糖尿病是一种代谢紊乱,影响中枢神经系统的发育,在学习和记忆中起着重要作用。糖尿病会增加细胞中的活性氧(ROS)水平并改变几种基因的表达,包括SYP,BDNF,PAX7和SYNCAM1,通过FOXO转录因子。这项研究旨在评估糖尿病对小鼠小脑皮质形态指标和基因表达的影响。
■在12名成年人中诱发了糖尿病,使用注射链脲佐菌素的雄性C57BL小鼠。两个月后,老鼠被解剖,小脑储存用于进一步分析。对于形态测量分析,组织切片用甲酚紫染色并用光学显微镜检查。对于基因表达分析,提取了RNA,并合成了cDNA。SYP的mRNA水平,BDNF,通过实时PCR方法测量PAX7和SYNCAM1基因。
■分子层和浦肯野层的厚度,且糖尿病组Purkinje和颗粒细胞数较对照组明显减少P<0.01)。该地区,周边,与对照组相比,糖尿病组Purkinje细胞的直径明显减小P<0.01)。PAX7、SYP、糖尿病组的BDNF基因显著降低。然而,糖尿病组大鼠小脑SYNCAM1表达较对照组显著升高(P<0.05)。
■诱导的小鼠糖尿病可以降低小脑中记忆相关基因的表达。此外,这些基因影响小脑的形态和厚度。
UNASSIGNED: Diabetes is a metabolic disorder that affects the development of the central nervous system and plays an important role in learning and memory. Diabetes increases the reactive oxygen species (ROS) level in cells and changes the expression of several genes, including SYP, BDNF,
PAX7, and SYNCAM1, through the FOXO transcription factor. This study was done to assess the effect of diabetes on morphometric indexes of the cerebellar cortex and gene expression in mice.
UNASSIGNED: Diabetes was induced in twelve adult, male C57BL mice using an injection of streptozotocin. After two months, the mice were dissected, and the cerebellum was stored for further analysis. For the morphometric analysis, tissue sections were stained with cresyl violet and examined with a light microscope. For gene expression analysis, the RNA was extracted, and cDNA was synthesized. The mRNA levels of SYP, BDNF,
PAX7, and SYNCAM1 genes were measured by the real-time PCR method.
UNASSIGNED: The thickness of the molecular layer and Purkinje layer, and the number of Purkinje and granular cells in the diabetic group were significantly reduced compared to controls P<0.0 1). The area, perimeter, and diameter of Purkinje cells in the diabetic group were significantly reduced compared to controls P<0.0 1). The expression of
PAX7, SYP, and BDNF genes of the diabetic group was significantly reduced. However, SYNCAM1 expression in the cerebellum of the diabetic group was significantly increased compared to controls (P<0.05).
UNASSIGNED: Induced diabetes in mice can decrease the expression of memory-related genes in the cerebellum. Also, these genes affect the morphology and thickness of the cerebellum.