PDF(Pubmed)
Abstract：
The principles of myogenesis play crucial roles in the production of cultured meat, and identifying protein stimulators associated with myogenesis holds great potential to enhance the efficiency of this process. In this study, we used surface plasmon resonance (SPR)-based screening of a natural product library to discover ligands for Pax7 and MyoD, key regulators of satellite cells (SCs), and performed cell-based assays on Hanwoo SCs (HWSCs) to identify substances that promote cell proliferation and/or differentiation. Through an SPR analysis, we found that six chemicals, including one Pax7+/MyoD- chemical, four Pax7+/MyoD+ chemicals, and one Pax7-/MyoD+ chemical, bound to Pax7 and/or MyoD proteins. Among four Pax7+/MyoD+ chemicals, parthenolide (0.5 and 1 µM) and rutin (100 and 200 µM) stimulated cell proliferation in the medium with 10% FBS similar to the medium with 20% FBS, without affecting differentiation. Adenosine, a Pax7-/MyoD+ chemical, accelerated differentiation. These chemicals could be potential additives to reduce the reliance of FBS required for HWSC proliferation and differentiation in cultured meat production.
摘要：
肌肉发生的原理在培养肉的生产中起着至关重要的作用，识别与肌生成相关的蛋白质刺激物具有提高该过程效率的巨大潜力。在这项研究中,我们使用基于表面等离子体共振（SPR）的天然产品库筛选来发现Pax7和MyoD的配体，卫星细胞（SC）的关键调节因子，并对HanwooSC（HWSC）进行基于细胞的测定，以鉴定促进细胞增殖和/或分化的物质。通过SPR分析,我们发现了六种化学物质，包括一种Pax7+/MyoD-化学物质，四种Pax7+/MyoD+化学品，还有一种Pax7-/MyoD+化学物质,与Pax7和/或MyoD蛋白结合。在四种Pax7+/MyoD+化学物质中，小白菊内酯（0.5和1µM）和芦丁（100和200µM）在含10％FBS的培养基中刺激细胞增殖，类似于含20％FBS的培养基，而不影响分化。腺苷,Pax7-/MyoD+化学物质，加速分化。这些化学物质可能是潜在的添加剂，以减少HWSC增殖和分化所需的FBS在养殖肉类生产中的依赖性。
