水凝胶,整合多种生物相容性材料,已经成为骨修复应用的有希望的候选人。本研究提出了一种用于骨组织工程的双网络水凝胶,结合聚(乙二醇)二丙烯酸酯(PEGDA)和壳聚糖(CS)通过UV聚合和离子交联交联。同时,使用一锅法合成了铜掺杂的介孔二氧化硅纳米球(Cu-MSNs)。Cu-MSNs通过原位生物矿化进行了额外的修饰,导致磷灰石层的形成。聚多巴胺用于促进钙(Ca)和磷酸盐(P)离子在Cu-MSNs(Cu-MSNs/PDA@CaP)表面的沉积。通过整合不同浓度的Cu-MSNs/PDA@CaP(25、50、100、150、200μg/mL)来产生复合水凝胶。表征揭示了复合水凝胶内独特的互连多孔结构,与纯PEGDA相比,压缩应力显着提高了169.6%(从89.01升高到240.19kPa)。体外生物相容性实验表明,复合水凝胶在7天内保持了提高的细胞活力(高达106.6%)并促进了细胞的快速增殖。水凝胶显示ALP表达的显著增加57.58%,ARS染色的惊人增加235.27%。此外,它显著增强了关键成骨基因的表达,如运行相关转录因子2(RUNX2),胶原蛋白1a1(Col1a1),和分泌磷蛋白1(Spp1),将其确立为有前途的骨再生支架。这项研究显示了Cu-MSNs/PDA@CaP如何成功地整合到双网络水凝胶中,产生具有良好生物反应的复合材料。由于其改进的特性,这种复合水凝胶具有推进骨再生程序的潜力。
Hydrogels, integrating diverse biocompatible materials, have emerged as promising candidates for bone repair applications. This study presents a double network hydrogel designed for bone tissue engineering, combining poly(ethylene glycol) diacrylate (
PEGDA) and chitosan (CS) crosslinked through UV polymerization and ionic crosslinking. Concurrently, copper-doped mesoporous silica nanospheres (Cu-MSNs) were synthesized using a one-pot method. Cu-MSNs underwent additional modification through in-situ biomineralization, resulting in the formation of an apatite layer. Polydopamine was employed to facilitate the deposition of Calcium (Ca) and Phosphate (P) ions on the surface of Cu-MSNs (Cu-MSNs/PDA@CaP). Composite hydrogels were created by integrating varied concentrations of Cu-MSNs/PDA@CaP (25, 50, 100, 150, 200 μg/mL). Characterization unveiled distinctive interconnected porous structures within the composite hydrogel, showcasing a notable 169.6 % enhancement in compressive stress (elevating from 89.01 to 240.19 kPa) compared to pure
PEGDA. In vitro biocompatibility experiments illustrated that the composite hydrogel maintained elevated cell viability (up to 106.6 %) and facilitated rapid cell proliferation over 7 days. The hydrogel demonstrated a substantial 57.58 % rise in ALP expression and a surprising 235.27 % increase in ARS staining. Moreover, it significantly enhanced the expression of crucial osteogenic genes, such as run-related transcription factors 2 (RUNX2), collagen 1a1 (Col1a1), and secreted phosphoprotein 1 (Spp1), establishing it as a promising scaffold for bone regeneration. This study shows how Cu-MSNs/PDA@CaP were successfully integrated into a double network hydrogel, resulting in a composite material with good biological responses. Due to its improved characteristics, this composite hydrogel holds the potential for advancing bone regeneration procedures.