具有以交换的抗原结合恒定结构域为特征的 Fab 臂的双特异性抗体。
关键词: Ab, antibody BLI, biolayer interferometry BSA, bovine serum albumin Bispecific antibody CDR, complementarity determining region DSC, differential scanning calorimetry Domain-exchanged antibody EC50, half-maximal effective concentration FBS, fetal bovine serum FITC, fluorescein isothiocyanate Fab constant domain exchange Fab, fragment antigen binding Fc, fragment crystallizable Fcab, Fc with antigen binding properties HPLC-SEC, high pressure liquid chromatography-size exclusion chromatography Her2 internalization IgG, immunoglobulin G LC-ESI-MS, liquid chromatography-electrospray ionization-mass spectrometry PBS, phosphate buffered saline PE, phycoerythrin PEI, polyethylenimine PNGase F, Peptide:N-glycosidase F RMSD, root mean square deviation TRA, trastuzumab Tm, melting temperature VEGF, vascular endothelial growth factor “Knobs-into-holes” heterodimerization
来 源: DOI:10.1016/j.bbrep.2021.100959 PDF(Sci-hub) PDF(Pubmed)