背景:枸橼酸西地那非对身体有各种影响,包括血管扩张,抑制血小板聚集,促进血管的生长,刺激细胞凋亡和成纤维细胞的粘附,减少炎症。这项研究旨在探讨西地那非如何影响手术治疗的跟腱,在组织结构和机械性能方面。
方法:将48只体重350~400g的Wistar白化大鼠随机分为两组,每组6人,研究组给予枸橼酸西地那非,对照组给予生理盐水,分别。在氯胺酮和赛拉嗪麻醉下建立跟腱断裂模型。在整个实验过程中,老鼠被安置在八个单独的笼子里,每人六个。第一组的研究组和对照组在1周结束时处死。并采集跟腱样本。之后,在14天处死第二组后采集跟腱样本,第三组在21天,第四组在28天,分别。新生血管形成,炎症,组织病理学评估收获的跟腱的纤维化和成纤维细胞活性。生物力学,对跟腱进行拉伸并持续直到肌腱破裂。计算破裂时的最大力值。
结果:给予枸橼酸西地那非21天的T21组的平均最大强度值,为31.1±4.36N,和对照组C21组的平均最大强度值,为20.56±6.92N。组间观察到显著差异(p:0.008)。在比较中,T28组(45.17±5.54N)也表现出比C28组(34.62±3.21N)更大的强度(p:0.004)。该研究还注意到两组之间新血管形成的显着差异,在第一周,1轻度,在T7组中观察到3个中度和2个显著的新生血管形成,在T28组中,在4个标本中观察到中度新生血管形成,并且在2个标本中观察到显著的新生血管形成(p:0.001)。此外,各组在纤维化水平上表现出显著差异,炎症和成纤维细胞增殖(分别为p:0.017,p:0.036,(p:0.035))。
结论:研究表明,枸橼酸西地那非可以增强肌腱愈合的生物力学和组织病理学方面,导致更强的肌腱。
BACKGROUND: Sildenafil Citrate has various effects on the body, including widening blood vessels, inhibiting platelet aggregation, promoting the growth of blood vessels, stimulating apoptosis and adhesion of fibroblasts, and reducing inflammation. This research aims to explore how Sildenafil Citrate affects surgically treated Achilles tendons, both in terms of tissue structure and mechanical properties.
METHODS: Forty-eight Wistar-albino rats weighing 350-400 g were randomly divided into groups, 6 in each group, as the study group was given Sildenafil Citrate and the control group given saline, respectively. The Achilles tendon rupture model was created under ketamine and xylazine anesthesia. During the entire experiment, rats were housed in eight separate cages, six of them each. The study group and control group of the first group were sacrificed at the end of 1 week, and Achilles tendon samples were taken. After that, Achilles tendon samples were taken after sacrificing the second group at 14 days, the third group at 21 days, and the fourth group at 28 days, respectively. Neovascularization, inflammation, fibrosis and fibroblastic activities of the harvested Achilles tendons were evaluated histopathologically. Biomechanically, stretching was applied to the Achilles tendons and continued until the tendon ruptured. the maximum force values at the moment of rupture were calculated.
RESULTS: The mean maximum strength value of group T21, which was given sildenafil citrate for 21 days, was 31.1 ± 4.36 N, and the mean maximum strength value of group C21, which was the control group, was 20.56 ± 6.92 N. A significant difference was observed between the groups (p: 0.008). Group T28 (45.17 ± 5.54 N) also demonstrated greater strength than group C28 (34.62 ± 3.21 N) in the comparison (p: 0.004). The study also noted significant differences between the groups in neovascularization, in the first week, 1 mild, 3 moderate and 2 prominent neovascularization was observed in group T7, in group T28, moderate neovascularization was observed in 4 specimens and prominent neovascularization was observed in 2 specimens (p: 0.001). Furthermore, the groups showed significant differences in their levels of fibrosis, inflammation and fibroblastic proliferation (p: 0.017, p: 0.036, (p: 0.035) respectively).
CONCLUSIONS: Study has demonstrated that sildenafil citrate can enhance the biomechanical and histopathological aspects of tendon healing, resulting in a stronger tendon.