Abstract：
BACKGROUND: Processing cow ghee (clarified butterfat) with therapeutic herbs, i.e. ghrita, is recognized for augmenting the therapeutic efficacy of plant materials. Ashwagandha ghrita (AG) is an effective Ayurvedic formulation consisting of Indian ginseng, i.e., Withania somnifera (L.) Dunal, the main constituent used to treat infertility, weakness, gynaecological disorders, and general debility.
OBJECTIVE: The present investigation was undertaken to corroborate the ethnopharmacological claim of AG as \'Vajikarana Rasayana\' for its aphrodisiac potential using bioinformatics (in-silico) and experimental (in-vitro and in-vivo) approaches.
METHODS: AG was formulated as per the methods reported in Ayurved sarsangraha. AG was further subjected to HPLC, GCMS analysis, and biological (acute toxicity and aphrodisiac) assessment per the standard procedures. Thirty-eight bioactives of Indian ginseng were subjected to computational studies (molecular docking and network pharmacology) to confirm the plausible mechanism.
RESULTS: AG was found to be safe up to 2000 mg/kg body wt., and it showed dose-dependent upsurge (p < 0.01 and p < 0.05, wherever necessary) in mount and intromission frequency, genital grooming, and anogenital sniffing at 150 and 300 mg/kg body weight suggesting aphrodisiac activity. In-vitro studies demonstrated significant relaxation of the Corpus Cavernosal Smooth Muscle at all concentrations in a dose-dependent manner. Furthermore, the results of molecular modelling studies were in agreement with the biological activity and showed interaction with phosphodiesterase-5 as a possible target.
CONCLUSIONS: AG exhibited an aphrodisiac effect and substantiated the traditional claim of Indian ginseng-based ghrita formulation as \'Vajikarana Rasayana\'.
OBJECTIVE: The present investigation was undertaken to corroborate the ethnopharmacological claim of AG as \'Vajikarana Rasayana\' for its aphrodisiac potential using bioinformatics (in-silico) and experimental (in-vitro and in-vivo) approaches.
METHODS: AG was formulated as per the methods reported in Ayurved sarsangraha. AG was further subjected to HPLC, GCMS analysis, and biological (acute toxicity and aphrodisiac) assessment per the standard procedures. Thirty-eight bioactives of Indian ginseng were subjected to computational studies (molecular docking and network pharmacology) to confirm the plausible mechanism.
RESULTS: AG was found to be safe up to 2000 mg/kg body wt., and it showed dose-dependent upsurge (p < 0.01 and p < 0.05, wherever necessary) in mount and intromission frequency, genital grooming, and anogenital sniffing at 150 and 300 mg/kg body weight suggesting aphrodisiac activity. In-vitro studies demonstrated significant relaxation of the Corpus Cavernosal Smooth Muscle at all concentrations in a dose-dependent manner. Furthermore, the results of molecular modelling studies were in agreement with the biological activity and showed interaction with phosphodiesterase-5 as a possible target.
CONCLUSIONS: AG exhibited an aphrodisiac effect and substantiated the traditional claim of Indian ginseng-based ghrita formulation as \'Vajikarana Rasayana\'.
摘要：
背景：用治疗草药加工牛酥油（澄清乳脂），即ghrita,被认为是增强植物材料的治疗功效。Ashwagandhaghrita（AG）是一种有效的阿育吠陀配方，由印度人参组成，即，有忧郁症（L.）Dunal是用于治疗不孕症的主要成分，弱点,妇科疾病,和普遍的虚弱。
目的：进行本研究是为了使用生物信息学（计算机模拟）和实验（体外和体内）方法来证实AG作为“VajikaranaRasayana”的壮阳潜力。
方法：AG按照Ayurvedsarsangraha报告的方法配制。AG进一步进行HPLC,GCMS分析，和生物（急性毒性和壮阳）评估按照标准程序。对印度人参的38种生物活性物质进行了计算研究（分子对接和网络药理学），以确认其合理的机制。
结果：发现AG在2000mg/kg体重下是安全的。,它在坐骑和入射频率上显示出剂量依赖性的上升（p<0.01和p<0.05，必要时），生殖器美容,和肛门生殖器嗅探在150和300毫克/千克体重表明壮阳活动。体外研究表明，在所有浓度下，海绵体平滑肌均以剂量依赖性方式显着松弛。此外，分子模型研究的结果与生物学活性结合在一起，并显示与磷酸二酯酶-5的相互作用是可能的靶标。
结论：AG表现出壮阳作用，并证实了印度人参为基础的ghrita制剂的传统主张为“VajikaranaRasayana”。
目的：进行本研究是为了使用生物信息学（计算机模拟）和实验（体外和体内）方法来证实AG作为“VajikaranaRasayana”的壮阳潜力。
方法：AG按照Ayurvedsarsangraha报告的方法配制。AG进一步进行HPLC,GCMS分析，和生物（急性毒性和壮阳）评估按照标准程序。对印度人参的38种生物活性物质进行了计算研究（分子对接和网络药理学），以确认其合理的机制。
结果：发现AG在2000mg/kg体重下是安全的。,它在坐骑和入射频率上显示出剂量依赖性的上升（p<0.01和p<0.05，必要时），生殖器美容,和肛门生殖器嗅探在150和300毫克/千克体重表明壮阳活动。体外研究表明，在所有浓度下，海绵体平滑肌均以剂量依赖性方式显着松弛。此外，分子模型研究的结果与生物学活性结合在一起，并显示与磷酸二酯酶-5的相互作用是可能的靶标。
结论：AG表现出壮阳作用，并证实了印度人参为基础的ghrita制剂的传统主张为“VajikaranaRasayana”。
