OBJECTIVE: The present investigation was undertaken to corroborate the ethnopharmacological claim of AG as \'Vajikarana Rasayana\' for its aphrodisiac potential using bioinformatics (in-silico) and experimental (in-vitro and in-vivo) approaches.
METHODS: AG was formulated as per the methods reported in Ayurved sarsangraha. AG was further subjected to HPLC, GCMS analysis, and biological (acute toxicity and aphrodisiac) assessment per the standard procedures. Thirty-eight bioactives of Indian ginseng were subjected to computational studies (molecular docking and network pharmacology) to confirm the plausible mechanism.
RESULTS: AG was found to be safe up to 2000 mg/kg body wt., and it showed dose-dependent upsurge (p < 0.01 and p < 0.05, wherever necessary) in mount and intromission frequency, genital grooming, and anogenital sniffing at 150 and 300 mg/kg body weight suggesting aphrodisiac activity. In-vitro studies demonstrated significant relaxation of the Corpus Cavernosal Smooth Muscle at all concentrations in a dose-dependent manner. Furthermore, the results of molecular modelling studies were in agreement with the biological activity and showed interaction with phosphodiesterase-5 as a possible target.
CONCLUSIONS: AG exhibited an aphrodisiac effect and substantiated the traditional claim of Indian ginseng-based ghrita formulation as \'Vajikarana Rasayana\'.
目的:进行本研究是为了使用生物信息学(计算机模拟)和实验(体外和体内)方法来证实AG作为“VajikaranaRasayana”的壮阳潜力。
方法:AG按照Ayurvedsarsangraha报告的方法配制。AG进一步进行HPLC,GCMS分析,和生物(急性毒性和壮阳)评估按照标准程序。对印度人参的38种生物活性物质进行了计算研究(分子对接和网络药理学),以确认其合理的机制。
结果:发现AG在2000mg/kg体重下是安全的。,它在坐骑和入射频率上显示出剂量依赖性的上升(p<0.01和p<0.05,必要时),生殖器美容,和肛门生殖器嗅探在150和300毫克/千克体重表明壮阳活动。体外研究表明,在所有浓度下,海绵体平滑肌均以剂量依赖性方式显着松弛。此外,分子模型研究的结果与生物学活性结合在一起,并显示与磷酸二酯酶-5的相互作用是可能的靶标。
结论:AG表现出壮阳作用,并证实了印度人参为基础的ghrita制剂的传统主张为“VajikaranaRasayana”。