Oropouche病毒(OROV)的特征是重新出现的虫媒病毒,引起了公众的极大关注,在拉丁美洲国家发现的几起急性发烧爆发的原因,登记了超过50万的报告病例。这种病毒的发病率本质上受到环境条件的青睐,其中这些特征与病媒种群向人口贩运地区的增加和分布有关。此外,在巴西,存在一个关于缺乏诊断的问题,该问题汇总了病原体的成功.因此,通过分子技术,我们发现了27例OROV阳性病例,在亚马逊西部的边境地区传播,其中44.44%(12/27)的队列特征为有报告症状的感染者,主要是发烧,肌痛,和背部疼痛。在阳性样本中,可以获得总共48.14%(13/27)的样品来分析Oropouche的S和M段,这显示了巴西序列之间的相似性。因此,有可能验证奥罗夫在朗多尼亚和边境地区的流通情况,其中跟踪被忽视的虫媒病毒对于新出现和重新出现的病毒的基因组监测是必要的。重要信息亚马逊西部地区以急性高热疾病的爆发而闻名,缺乏针对不同病原体的特定诊断阻碍了医疗保健单位对患者的管理。1990年代该地区已经记录了Oropouche病毒。然而,这是第一项研究,在这个记录之后,使用筛查测试来排除寨卡病毒,以检测患有急性高热疾病的个体,登革热,还有基孔肯雅,通过对Rondonia州和边境地区的病毒传播进行测序来证实。我们强调包括对Oropouche等病毒进行诊断的重要性,多年来一直在遭受漏报,并且与亚马逊西部地区的季节性相关,对该地区的公共卫生有直接影响的因素。此外,我们强调基因组监测在阐明影响这些地区常住人口的暴发中的重要性。
Oropouche virus (OROV) is characterized as a re-emerging arbovirus of great concern for public health, being responsible for several outbreaks of acute fever identified in Latin American countries, registering more than half a million reported cases. The incidence of reports of this virus is intrinsically favored by environmental conditions, in which such characteristics are related to the increase and distribution of the vector population to areas of human traffic. Moreover, there is a problem regarding the lack of diagnosis in Brazil that aggregates the success of the etiologic agent. Thus, by means of molecular techniques, we identified 27 positive cases of the OROV circulating in border locations in western Amazon, with 44.44% (12/27) of the cohort characterized as infected individuals with reported symptoms, mainly ranging from fever, myalgia, and back pain. Among the positive samples, it was possible to obtain a total of 48.14% (13/27) samples to analyze the S and M segments of
Oropouche, which showed similarities among the Brazilian sequences. Thus, it was possible to verify the circulation of the OROV in Rondonia and border areas, in which the tracking of neglected arboviruses is necessary for the genomic surveillance of emerging and re-emerging viruses.IMPORTANCEThe western Amazon region is known for outbreaks of acute febrile illnesses, to which the lack of specific diagnostics for different pathogens hinders the management of patients in healthcare units. The
Oropouche virus has already been recorded in the region in the 1990s. However, this is the first study, after this record, to perform the detection of individuals with acute febrile illness using a screening test to exclude Zika, dengue, and chikungunya, confirmed by sequencing the circulation of the virus in the state of Rondonia and border areas. We emphasize the importance of including diagnostics for viruses such as
Oropouche, which suffers underreporting for years and is related to seasonal periods in Western Amazon locations, a factor that has a direct influence on public health in the region. In addition, we emphasize the importance of genomic surveillance in the elucidation of outbreaks that affect the resident population of these locations.