目的:小熊猫目前是Caniformaia亚目中唯一幸存的Ailuidae家族成员。在这项研究中,我们提供解剖学数据,形态计量学,眶区的组织学和组织化学检查,眼睑,眶腺,和来自弗罗茨瓦夫动物园的两名成年男性Ailurusfulgensfulgens的眼衣,波兰。
方法:该研究涉及眼球和选定的眼睛附属器官的形态计量学分析,随着骨骼轨道的分析,包括它的形态计量学,宏观,和微观评估。显微镜评估包括组织学和组织化学染色,前者涉及苏木精和伊红(H&E),Movatpentachrome,Picro-Mallory三色,Fontana-Masson,后者包括PAS,ABpH1.0,ABpH2.5;ABpH2.5/PAS,HDI。
结果:上眼睑(UE)和下眼睑(LE)表现出发育良好的骶骨腺,皮脂腺,和特征性的简单肺泡腺(产生粘液分泌)。存在泪腺的眼睑部分。仅在上眼睑观察到单个淋巴滤泡。第三眼睑(SGTE)的浅表腺体是多叶腺泡复合物,可产生粘液分泌,并包含在许多淋巴结聚集体的小叶间导管内。第三眼睑(TE)呈T形,由透明组织组成,含有ALT。泪腺(LG)还显示出多叶腺泡复合物,产生粘液分泌,只有一个淋巴滤泡.角膜由4层组成,因为鲍曼的膜不存在。证明了由7-10层上皮细胞组成的Vogt栅栏。瞳孔在静止时水平呈卵形(死后)。括约肌瞳孔和扩张器瞳孔发育良好。宏观上,透明绒毡层表现为乳白色,非乳白色的新月形。组织学上,脉络膜透明绒毡层由5至9层松散堆积的卵圆细胞组成。视网膜的组成与陆地夜间食肉动物的组成相似。
结论:我们的研究结果表明,小熊猫的眼睛和眼眶区域的解剖特征与Musteloidea分支中描述的相似,以及犬科和乌尔西科。
OBJECTIVE: The red panda is currently the only surviving member of the Ailuridae family in the Caniformia suborder. In this study, we provide data on anatomical, morphometric, histological and histochemical examination of the orbital region, eyelids, orbital gland, and eye tunics in two adult males Ailurus fulgens fulgens from the Wroclaw Zoological Garden, Poland.
METHODS: The study involved morphometric analysis of the eyeball and selected accessory organs of the eye, along with analysis of the bony
orbit, including its morphometry, macroscopic, and microscopic evaluation. Microscopic evaluation encompassed histological and histochemical staining, with the former involving hematoxylin & eosin (H&E), Movat pentachrome, picro-Mallory trichrome, Fontana-Masson, and the latter including PAS, AB pH 1.0, AB pH 2.5; AB pH 2.5/PAS, and HDI.
RESULTS: The upper (UE) and lower (LE) eyelids presented well-developed tarsal glands, sebaceous glands, and a characteristic simple alveolar gland (producing a mucous secretion). The palpebral part of the lacrimal gland was present. A single lymphoid follicle was observed only in the upper eyelids. The superficial gland of the third eyelid (SGTE) was a multilobar acinar complex that produces mucous secretion and is contained within the interlobular ducts of numerous aggregates of lymph nodes. The third eyelid (TE) was T-shaped and composed of hyaline tissue, containing CALT. The lacrimal gland (LG) also revealed a multilobar acinar complex that produced mucous secretion, with a single lymphoid follicle. The cornea consisted of 4 layers, as Bowman\'s membrane was absent. The Vogt palisades composed of 7-10 layers of epithelial cells were demonstrated. The pupil was horizontally ovoid at rest (post-mortem). The sphincter pupil and the dilator pupil were well developed. Macroscopically, the tapetum lucidum appeared as a milky, non-opalescent crescent. Histologically, the choroidal tapetum lucidum cellulosum consisted of 5 to 9 layers of loosely packed oval cells. The retina showed a composition similar to that of terrestrial nocturnal carnivores.
CONCLUSIONS: The results of our research indicate that the anatomical features of the eye and orbital region in the red panda share similarities with those described in the Musteloidea clade, as well as the Canidae and Ursidae families.