■为了评估最常见的口腔病原体的粘附水平的差异,变形链球菌和牙龈卟啉单胞菌,在人类唾液来源的微观世界生物膜中,与时间和正畸托槽的原材料有关。
■将样品分为三组支架材料:1)单晶氧化铝陶瓷(CR),2)不锈钢金属(SS),和3)聚碳酸酯塑料(PL),和羟基磷灰石(HA)组用于模拟牙釉质表面。唾液是从一个健康的捐赠者那里收集的,和唾液衍生的生物膜在每个样品上生长。进行实时聚合酶链反应以定量评估总细菌附着水平的差异,变形链球菌和牙龈卟啉单胞菌在第1天和第4天。
■变形链球菌和牙龈卟啉单胞菌对CR和HA的粘附力高于其他支架材料(SS=PL■口腔病原体的粘附性越高,如变形链球菌和牙龈卟啉单胞菌对CR的影响表明,在正畸治疗期间,使用CR托槽可能会促进牙龈炎症和牙釉质脱钙。
UNASSIGNED: To evaluate differences in the adhesion levels of the most common oral pathogens, Streptococcus mutans and Porphyromonas gingivalis , in human saliva-derived microcosm biofilms with respect to time and raw materials of orthodontic brackets.
UNASSIGNED: The samples were classified into three groups of bracket materials: 1) monocrystalline alumina ceramic (CR), 2) stainless steel metal (SS), and 3) polycarbonate plastic (PL), and a hydroxyapatite (HA) group was used to mimic the enamel surface. Saliva was collected from a healthy donor, and saliva-derived biofilms were grown on each sample. A real-time polymerase chain reaction was performed to quantitatively evaluate differences in the attachment levels of total bacteria, S. mutans and P. gingivalis at days 1 and 4.
UNASSIGNED: Adhesion of S. mutans and P. gingivalis to CR and HA was higher than the other bracket materials (SS = PL < CR = HA). Total bacteria demonstrated higher adhesion to HA than to bracket materials, but no significant differences in adhesion were observed among the bracket materials (CR = SS = PL < HA). From days 1 to 4, the adhesion of P. gingivalis decreased, while that of S. mutans and total bacteria increased, regardless of material type.
UNASSIGNED: The higher adhesion of oral pathogens, such as S. mutans and P. gingivalis to CR suggests that the use of CR brackets possibly facilitates gingival inflammation and enamel decalcification during orthodontic treatment.