Abstract：
Colonization by several oral pathogens and the onset of oral diseases, such as dental caries and periodontal diseases, are closely related. Therefore, the analysis of pathogens in oral specimens would be helpful for the risk assessment of oral diseases. We developed a rapid multiplex real-time polymerase chain reaction (PCR) method using a portable device and newly designed probe/primer sets to detect the oral pathogens Streptococcus mutans, Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia. The theoretical minimum detectable cell numbers of S. mutans, P. gingivalis, T. denticola, and T. forsythia were 1, 1, 4, and 3, respectively. The multiplex real-time PCR system simultaneously detected the colonization of S. mutans and P. gingivalis in human saliva. These results suggest that the multiplex real-time PCR system may be useful for the risk assessment of oral diseases.
摘要：
几种口腔病原体的定植和口腔疾病的发作，如龋齿和牙周疾病，密切相关。因此,口腔标本中病原体的分析将有助于口腔疾病的风险评估。我们开发了一种快速多重实时聚合酶链反应（PCR）方法，使用便携式设备和新设计的探针/引物组来检测口腔病原体变异链球菌，牙龈卟啉单胞菌,Denticola密螺旋体,还有连翘坦纳菌.变异链球菌的理论最小可检测细胞数，牙龈卟啉单胞菌，T.Denticola,连翘和连翘分别为1、1、4和3。多重实时PCR系统同时检测人唾液中变异链球菌和牙龈卟啉单胞菌的定植。这些结果表明，多重实时PCR系统可能有助于口腔疾病的风险评估。
