■口腔微生物群的失衡可导致各种口腔疾病,并可能影响总体健康。长期饮酒超过一定阈值可能会影响牙周炎的发作和进展。然而,慢性饮酒影响牙周炎的机制及其与口腔微生物群落变化的关系尚不清楚.
■这项研究使用16SrRNA基因扩增子测序来检查受慢性饮酒影响的牙周炎大鼠口腔微生物群落的动态变化。
■将24只雄性Wistar大鼠随机分配到牙周炎(P)或牙周炎酒精(PA)组。PA组可以不受限制地获得酒精10周,而P组只能获得水。四周后,两组均出现牙周炎。10周后,测定大鼠血清中谷丙转氨酶和谷草转氨酶的血清水平。口腔拭子取自大鼠,并进行16SrRNA基因测序。使用苏木精和曙红染色和显微计算机断层扫描评估牙槽骨状态。
■与牙周炎组相比,PA组的大鼠表现出更严重的牙周组织损伤。虽然口腔微生物多样性保持稳定,两组之间某些微生物群落的相对丰度存在显着差异。在PA组中,放线菌和脱硫菌在门水平更为普遍。在属一级,Cutubacterium,提西菌,Romboutsia,放线菌,Lawsonella,缺氧球菌,和梭状芽孢杆菌_sensu_stricto_1在PA组中明显更丰富,而嗜血杆菌明显不那么丰富。此外,使用Tax4Fun进行功能预测显示,PA组的碳水化合物代谢显着丰富。
■长期饮酒会加剧大鼠牙周炎,并影响其口腔微生物群的组成和功能特征,如16SrRNA基因测序结果所示。由于长期饮酒,这些微生物改变可能导致大鼠牙周炎恶化。
UNASSIGNED: The imbalance of oral microbiota can contribute to various oral disorders and potentially impact general health. Chronic alcohol consumption beyond a certain threshold has been implicated in influencing both the onset and progression of periodontitis. However, the mechanism by which chronic alcohol consumption affects periodontitis and its association with changes in the oral microbial community remains unclear.
UNASSIGNED: This study used 16S rRNA gene amplicon sequencing to examine the dynamic changes in the oral microbial community of rats with periodontitis influenced by chronic alcohol consumption.
UNASSIGNED: Twenty-four male Wistar rats were randomly allocated to either a periodontitis (P) or periodontitis + alcohol (PA) group. The PA group had unrestricted access to alcohol for 10 weeks, while the P group had access to water only. Four weeks later, both groups developed periodontitis. After 10 weeks, serum levels of alanine aminotransferase and aspartate aminotransferase in the rats\' serum were measured. The oral swabs were obtained from rats, and 16S rRNA gene sequencing was conducted. Alveolar bone status was assessed using hematoxylin and eosin staining and micro-computed tomography.
UNASSIGNED: Rats in the PA group exhibited more severe periodontal tissue damage compared to those in the periodontitis group. Although oral microbial diversity remained stable, the relative abundance of certain microbial communities differed significantly between the two groups. Actinobacteriota and Desulfobacterota were more prevalent at the phylum level in the PA group. At the genus level, Cutibacterium, Tissierella, Romboutsia, Actinomyces, Lawsonella, Anaerococcus, and Clostridium_sensu_stricto_1 were significantly more abundant in the PA group, while Haemophilus was significantly less abundant. Additionally, functional prediction using Tax4Fun revealed a significant enrichment of carbohydrate metabolism in the PA group.
UNASSIGNED: Chronic alcohol consumption exacerbated periodontitis in rats and influenced the composition and functional characteristics of their oral microbiota, as indicated by 16S rRNA gene sequencing results. These microbial alterations may contribute to the exacerbation of periodontitis in rats due to chronic alcohol consumption.