碱性亮氨酸拉链域转录因子(TFs),其中酵母激活蛋白(Yap)是一个重要的类别,对菌核的发展至关重要,应激反应,营养生长,和孢子粘附。然而,关于YapTFs如何对昆虫病原真菌的致病性没有任何了解。在这项工作中,球孢白僵菌用于鉴定和敲除与Yap相似的酵母基因BbYap1。BbYap1基因缺失对球孢芽孢杆菌的脂质稳态有影响;油酸,例如,下降了95.69%。与野生菌株相比,BbYap1突变体的毒力和营养性发育要少得多,同时对化学压力表现出更大的敏感性。值得注意的是,BbYap1缺失带来的生理异常在很大程度上是通过添加外源油酸来修复的,如在血腔注射组中昆虫存活率的显著增加所见。感染BbYap1突变体后,宿主表现出β-1,3-葡聚糖识别蛋白表达的显著下调,gallerimycin,gloverin,和类似于moricin的蛋白质基因.同样,外源油酸的引入显著增加了宿主对上述基因的表达。总之,BbYap1通过逃避宿主体液防御来调节细胞酶脂质稳态和真菌毒力,这有助于真菌化学胁迫和营养发育。
目的:昆虫病原真菌(EPF)在生物防治策略中提供了一种有效且生态友好的替代方法来遏制昆虫种群。当EPF进入宿主的血淋巴时,他们会遇到各种各样的应激反应,如免疫和氧化应激。碱性亮氨酸拉链域转录因子,其中酵母激活蛋白(Yap)是一个重要的类别,具有与新陈代谢相关的多种生物学功能,发展,繁殖,分生孢子,应激反应,和致病性。这项研究表明,球孢白僵菌的BbYap1通过逃避宿主体液防御来调节细胞酶脂质稳态和真菌毒力,这有助于真菌化学胁迫和营养发育。这些发现为理解YAP在EPF中的分子作用提供了新的视角。
Basic leucine zipper domain transcription factors (TFs), of which yeast activator protein (Yap) is a significant class, are crucial for the development of sclerotia, the stress response, vegetative growth, and spore adhesion. Nevertheless, nothing is known about how Yap TFs contribute to the pathogenicity of entomopathogenic fungus. In this work, Beauveria bassiana was used to identify and knock out the yeast gene BbYap1, which is similar to Yap. The BbYap1 gene deletion has an impact on lipid homeostasis of B. bassiana; oleic acid, for example, dropped by 95.69%. The BbYap1 mutant exhibited much less virulence and vegetative development in comparison to the wild strain, while demonstrating a greater sensitivity to chemical stress. It is noteworthy that the physiological abnormalities brought on by BbYap1 deletion were largely repaired by the addition of exogenous oleic acid, as seen by the notable increase in insect survival in the blood cavity injection group. Following infection with the BbYap1 mutant, the host exhibits a considerable down-regulation of the expression of β-1,3-glucan recognition protein, gallerimycin, gloverin, and moricin-like protein genes. Likewise, the introduction of exogenous oleic acid markedly increased the host\'s expression of the aforementioned genes. In summary, BbYap1 regulates cellular enzyme lipid homeostasis and fungal virulence by eluding host humoral defense, which contributes to fungal chemical stress and vegetative development.
OBJECTIVE: Entomopathogenic fungi (EPF) offer an effective and eco-friendly alternative to curb insect populations in biocontrol strategy. When EPF enter the hemolymph of their host, they encounter a variety of stress reactions, such as immunological and oxidative stress. Basic leucine zipper domain transcription factors, of which yeast activator protein (Yap) is a significant class, have diverse biological functions related to metabolism, development, reproduction, conidiation, stress responses, and pathogenicity. This study demonstrates that BbYap1 of Beauveria bassiana regulates cellular enzyme lipid homeostasis and fungal virulence by eluding host humoral defense, which contributes to fungal chemical stress and vegetative development. These findings offer fresh perspectives for comprehending molecular roles of YAP in EPF.