AP2/ERF转录因子基因在调节植物对各种非生物胁迫的反应中起着重要作用,如寒冷,干旱,高盐度,和高温。然而,对油棕AP2/ERF基因的功能了解较少。我们先前获得了172个油棕的AP2/ERF基因,发现在盐度下,EgAP2.25的表达显着上调,冷,或干旱胁迫条件。在本研究中,进行了EgAP2.25的序列鉴定和表达分析,表明它在烟草中瞬时过表达。结果表明,过表达EgAP2.25的转基因烟草植物比野生型烟草植物对盐度胁迫具有更强的耐受性。与野生型植物相比,过表达系显示出较高的发芽率,更好的植物生长,和更少的叶绿素损害。此外,EgAP2.25转基因植物耐盐性的提高主要归因于较高的抗氧化酶活性,脯氨酸和可溶性糖含量增加,减少H2O2的产生,和较低的MDA积累。此外,几个与胁迫相关的标记基因,包括NtSOD,NtPOD,NtCAT,NtERD10B,NtDREB2B,NtERD10C,和NtP5CS,在受盐分胁迫的EgAP2.25转基因烟草植株中显著上调。总的来说,EgAP2.25基因的过表达显着增强了转基因烟草植物的盐度胁迫耐受性。本研究为进一步探索EgAP2.25基因赋予油棕耐盐性的调控机制奠定了基础。
AP2/ERF transcription factor genes play an important role in regulating the responses of plants to various abiotic stresses, such as cold, drought, high salinity, and high temperature. However, less is known about the function of oil palm AP2/ERF genes. We previously obtained 172 AP2/ERF genes of oil palm and found that the expression of EgAP2.25 was significantly up-regulated under salinity, cold, or drought stress conditions. In the present study, the sequence characterization and expression analysis for EgAP2.25 were conducted, showing that it was transiently over-expressed in Nicotiana tabacum L. The results indicated that transgenic tobacco plants over-expressing EgAP2.25 could have a stronger tolerance to salinity stress than wild-type tobacco plants. Compared with wild-type plants, the over-expression lines showed a significantly higher germination rate, better plant growth, and less chlorophyll damage. In addition, the improved salinity tolerance of EgAP2.25 transgenic plants was mainly attributed to higher antioxidant enzyme activities, increased proline and soluble sugar content, reduced H2O2 production, and lower MDA accumulation. Furthermore, several stress-related marker genes, including NtSOD, NtPOD, NtCAT, NtERD10B, NtDREB2B, NtERD10C, and NtP5CS, were significantly up-regulated in EgAP2.25 transgenic tobacco plants subjected to salinity stress. Overall, over-expression of the EgAP2.25 gene significantly enhanced salinity stress tolerance in transgenic tobacco plants. This study lays a foundation for further exploration of the regulatory mechanism of the EgAP2.25 gene in conferring salinity tolerance in oil palm.