Abstract：
<b>Background and Objective:</b> The prioritisation of oil palm studies involves the exploration of novel bacterial isolates as possible agents for suppressing <i>Ganoderma boninense</i>. The objective of this study was to evaluate and characterise the potential of rhizospheric bacteria, obtained from the rhizosphere of oil palm plants, in terms of their ability to demonstrate anti-<i>Ganoderma </i>activity. <b>Materials and Methods:</b> The study began by employing a dual culture technique to select hostile bacteria. Qualitative detection was performed to assess the antifungal activity, as well as the synthesis of chitinase and glucanase, from certain isolates. The candidate strains were molecularly identified using 16S-rRNA ribosomal primers, specifically the 27F and 1492R primers. <b>Results:</b> The findings of the study indicated that the governmental plantation exhibited the highest ratio between diazotroph and indigenous bacterial populations in comparison to the other sites. Out of a pool of ninety bacterial isolates, a subset of twenty-one isolates demonstrated the ability to impede the development of <i>G. boninense</i>, as determined using a dual culture experiment. Twenty-one bacterial strains were found to exhibit antifungal activity. Nine possible bacteria were found based on the sequence analysis. These bacteria include <i>Burkholderia territorii</i> (RK2, RP2, RP3, RP5), <i>Burkholderia stagnalis</i> (RK3), <i>Burkholderia cenocepacia</i> (RP1), <i>Serratia marcescens</i> (RP13) and <i>Rhizobium multihospitium</i> (RU4). <b>Conclusion:</b> The findings of the study revealed that a significant proportion of the bacterial population exhibited the ability to perform nitrogen fixation, indole-3-acetic acid (IAA) production and phosphate solubilization. However, it is worth noting that <i>Rhizobium multihospitium</i> RU4 did not demonstrate the capacity for phosphate solubilization, while <i>B. territory</i> RK2 did not exhibit IAA production.
摘要：
<b>背景和目标:</b>油棕研究的优先次序包括探索新型细菌分离株作为抑制<i>灵芝boninense</i>的可能药物。这项研究的目的是评估和表征根际细菌的潜力，从油棕榈植物的根际获得，就他们展示抗灵芝和灵芝活动的能力而言。<b>材料与方法:</b>本研究开始于采用双重培养技术选择敌对细菌。进行定性检测以评估抗真菌活性，以及几丁质酶和葡聚糖酶的合成，从某些分离物中。使用16S-rRNA核糖体引物对候选菌株进行分子鉴定，特别是27F和1492R引物。＆lt;b＆gt;结果：＆lt;/b＆gt;研究结果表明，与其他地点相比，政府种植园在重氮菌和土著细菌种群之间的比例最高。在九十种细菌分离物中，21个分离株的一个子集证明了阻碍<i>G.发展的能力。boninense</i>,如使用双重培养实验所确定的。发现21种细菌菌株具有抗真菌活性。根据序列分析发现了9种可能的细菌。这些细菌包括<i>伯克霍尔德氏菌</i>(RK2,RP2,RP3,RP5),<i>Burkholderiastagnalis</i>(RK3),<i>伯克霍尔德氏菌</i>(RP1),<i>粘质沙雷氏菌</i>(RP13)和<i>多菌根瘤菌</i>(RU4).<b>结论:</b>研究结果表明,相当比例的细菌种群表现出进行固氮的能力,吲哚-3-乙酸(IAA)生产和磷酸盐溶解。然而,值得注意的是，<i>多菌根瘤菌</i>RU4没有证明磷酸盐溶解的能力，而<i>B.领土</i>RK2没有展示IAA生产。
