Occurrence

发生
  • 文章类型: Journal Article
    抗生素污染由于其对公共卫生和环境的深远影响而引起全球关注。这篇全面的综述探讨了环境污染中各种抗生素类别的普遍存在及其与自然生态系统的相互作用。氟喹诺酮类药物,大环内酯类,四环素,和磺酰胺已成为全球环境基质中普遍存在的污染物。这些抗生素的浓度在不同的环境中有所不同,受生产实践的影响,消费者行为,和社会经济因素。低收入和中低收入国家在管理抗生素污染方面面临着独特的挑战。具有水解等主导机制,吸附,和生物降解导致有毒副产物的形成。生态毒性报告揭示了这些副产物对水生和陆地生态系统的有害影响,进一步强调问题的严重性。值得注意的是,单独监测抗生素母体化合物可能不足以制定有效的抗生素污染控制和管理策略。这一审查强调了全面、解决环境抗生素污染和对抗抗生素耐药性的多部门方法。它还主张制定和实施考虑到具体环境条件和因素的量身定制的国家行动计划。因此,一种方法对于维护公众健康和自然生态系统的微妙平衡至关重要。
    Antibiotic contamination poses a significant global concern due to its far-reaching impact on public health and the environment. This comprehensive review delves into the prevalence of various antibiotic classes in environmental pollution and their interactions with natural ecosystems. Fluoroquinolones, macrolides, tetracyclines, and sulphonamides have emerged as prevalent contaminants in environmental matrices worldwide. The concentrations of these antibiotics vary across diverse environments, influenced by production practices, consumer behaviours, and socio-economic factors. Low- and low-middle-income countries face unique challenges in managing antibiotic contamination, with dominant mechanisms like hydrolysis, sorption, and biodegradation leading to the formation of toxic byproducts. Ecotoxicity reports reveal the detrimental effects of these byproducts on aquatic and terrestrial ecosystems, further emphasizing the gravity of the issue. Notably, monitoring the antibiotic parent compound alone may be inadequate for framing effective control and management strategies for antibiotic pollution. This review underscores the imperative of a comprehensive, multi-sectoral approach to address environmental antibiotic contamination and combat antimicrobial resistance. It also advocates for the development and implementation of tailored national action plans that consider specific environmental conditions and factors. Thus, an approach is crucial for safeguarding both public health and the delicate balance of our natural ecosystems.
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  • 文章类型: Journal Article
    在可预见的将来,由于污染物高氯酸盐的出现而产生的污染预计将对水环境产生重大影响。大量的研究工作已经致力于研究解决高氯酸盐污染的处理技术,近几十年来引起了广泛的国际关注。利用科学网进行了系统评价,Scopus,和科学直接数据库,以确定2000年至2024年发表的相关文章。总共选择了551篇文章用于利用VOS查看器进行深入检查。文献计量分析表明,中国等国家,美国,智利,印度,Japan,韩国一直是这一主题研究的重要贡献者。ClO4-的发生顺序为:地表水>地下水>饮用水。高氯酸盐污染的各种修复方法,如吸附,离子交换,膜过滤,化学还原,和生物还原,有人建议。此外,该研究严格评估了每种方法的优缺点,并提出了解决其局限性的建议。先进的技术已经显示出显著提高从水源中去除高氯酸盐和共污染物的潜力。然而,为了有效去除水中的高氯酸盐,需要解决天然水源中高氯酸盐浓度低和与这些技术相关的能耗高的问题。
    The pollution resulting from the emergence of the contaminant perchlorate is anticipated to have a substantial effect on the water environment in the foreseeable future. Considerable research efforts have been devoted to investigating treatment technologies for addressing perchlorate contamination, garnering widespread international interest in recent decades. A systematic review was conducted utilizing the Web of Science, Scopus, and Science Direct databases to identify pertinent articles published from 2000 to 2024. A total of 551 articles were chosen for in-depth examination utilizing VOS viewer. Bibliometric analysis indicated that countries such as China, the United States, Chile, India, Japan, and Korea have been prominent contributors to the research on this topic. The order of ClO4- occurrence was as follows: surface water > groundwater > drinking water. Various remediation methods for perchlorate contamination, such as adsorption, ion-exchange, membrane filtration, chemical reduction, and biological reduction, have been suggested. Furthermore, the research critically evaluated the strengths and weaknesses of each approach and offered recommendations for addressing their limitations. Advanced technologies have shown the potential to achieve notably enhanced removal of perchlorate and co-contaminants from water sources. However, the low concentration of perchlorate in natural water sources and the high energy consumption related to these technologies need to be solved in order to effectively remove perchlorate from water.
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  • 文章类型: Journal Article
    水生环境中新兴微污染物(EM)的累积加剧,特别是在COVID-19之后,引起了全世界对安全问题的极大关注。本文综述了废水中25种抗COVID-19相关EMs的来源和发生情况。应该指出的是,抗COVID-19相关EM的浓度,比如抗病毒药物,增塑剂,抗菌药物,大流行后,废水中的精神药物显着增加。此外,生态毒性,生态,重点比较和分析COVID-19前后典型EMs的健康风险。基于环境健康优先指数法,确定了与抗COVID-19相关的典型EMs的优先控制序列。洛匹那韦(LPV),文拉法辛(VLX),邻苯二甲酸二(2-乙基己基)酯(DEHP),苯扎氯铵(BAC),三氯卡班(TCC),邻苯二甲酸二正丁酯(DBP),西酞普兰(CIT),邻苯二甲酸二异丁酯(DIBP),和三氯生(TCS)被确定为大流行后时期的重中之重。此外,还提供了一些有关EMs毒性和风险评估的见解。该综述为正确认识和控制COVID-19后的EMs污染提供了方向,对客观评估COVID-19对环境和健康的影响具有重要意义。
    Aggravated accumulation of emerging micropollutants (EMs) in aquatic environments, especially after COVID-19, raised significant attention throughout the world for safety concerns. This article reviews the sources and occurrence of 25 anti-COVID-19 related EMs in wastewater. It should be pointed out that the concentration of anti-COVID-19 related EMs, such as antivirals, plasticizers, antimicrobials, and psychotropic drugs in wastewater increased notably after the pandemic. Furthermore, the ecotoxicity, ecological, and health risks of typical EMs before and after COVID-19 were emphatically compared and analyzed. Based on the environmental health prioritization index method, the priority control sequence of typical EMs related to anti-COVID-19 was identified. Lopinavir (LPV), venlafaxine (VLX), di(2-ethylhexyl) phthalate (DEHP), benzalkonium chloride (BAC), triclocarban (TCC), di-n-butyl phthalate (DBP), citalopram (CIT), diisobutyl phthalate (DIBP), and triclosan (TCS) were identified as the top-priority control EMs in the post-pandemic period. Besides, some insights into the toxicity and risk assessment of EMs were also provided. This review provides direction for proper understanding and controlling the EMs pollution after COVID-19, and is of significance to evaluate objectively the environmental and health impacts induced by COVID-19.
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  • 文章类型: Journal Article
    长时间接触多环芳烃(PAHs)及其衍生物,特别是硝化多环芳烃(NPAHs)和含氧多环芳烃(OPAHs),与单独的PAHs相比,可能会导致不良健康影响,并且可能具有更高的毒性风险。各种提取方法已用于来自食品样品的PAHs衍生物。然后使用气相色谱/质谱和高效液相色谱技术分析分析物。PAHs衍生物越来越多地在环境中被检测到,引发了众多研究人员的审查。同样,他们在食物中的存在正成为一个重要的问题。在熏制食品中发现的PAH衍生物水平升高可能导致有害的饮食暴露并造成潜在的健康危害。此外,调查食物中这些污染物的暴露水平是当务之急,因为它们被人类消费具有固有的风险。因此,这篇综述集中在毒性,分析,发生,以及食物来源中存在的NPAHs和OPAHs的风险评估。
    Prolonged exposure to polycyclic aromatic hydrocarbons (PAHs) and their derivatives, particularly nitrated polycyclic aromatic hydrocarbons (NPAHs) and oxygenated polycyclic aromatic hydrocarbons (OPAHs), can result in adverse health effects and may carry higher toxicity risks compared to PAHs alone. Various extraction methods have been utilized for PAHs derivatives from food samples. The analytes are then analyzed using gas chromatography/mass spectrometry and high-performance liquid chromatography techniques. PAHs derivatives are increasingly being detected in the environment, prompting scrutiny from numerous researchers. Similarly, their presence in food is becoming a significant concern. The elevated levels of PAH derivatives found in smoked food may result in detrimental dietary exposure and pose potential health hazards. Furthermore, investigating the level of exposure to these contaminants in food is imperative, as their consumption by humans carries inherent risks. Consequently, this review concentrates on the toxicity, analysis, occurrence, and risk evaluation of NPAHs and OPAHs present in food sources.
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  • 文章类型: Journal Article
    西藏东南部的墨脱是各种各样的野生动物的家园。然而,研究这些哺乳动物的物种目录,分布,保护状况仍然不足,尽管它们在维持生态平衡方面发挥了关键作用。这项研究进行了一项相机诱捕调查,以评估哺乳动物的生物多样性以及在格当的自然栖息地中保护哺乳动物的重要性,墨狗还讨论了藏东南山区野生动物保护研究的未来方向和应用前景。调查,从2023年4月到2024年5月,有19,754个相机陷阱天,揭示了5个目14科的25种哺乳动物。其中,四个被列为濒危物种,五个是脆弱的,两个被IUCN红色名录威胁到了,有9人在中国脊椎动物红色名录上被列为极度濒危或濒危,七种是中国国家一级重点保护野生动物。食肉目表现出最高的多样性,包括12种。此外,这项研究填补了有关IUCN中贡山muntjacMuntiacusgongshanensis代表性不足的知识空白,并首次为雅鲁藏布江沿岸的喜马拉雅红熊猫Ailurusfulgens和中国红熊猫Ailurusstyani的共存记录提供了新的见解,并记录了四个大中型物种的新海拔上限。关于相机陷阱捕获的相对丰度指数(RAI),确定的最普遍的物种是白颊猕猴,其次是贡山蒙太奇和喜马拉雅山塞罗·卡比尼斯塔尔。监测还捕获了一些家犬和牲畜,以及人类的干扰。这些发现强调了保护这些哺乳动物的重要性,并强调了保护其栖息地和减少威胁其生存的人类活动的必要性。从而维持该地区的生态平衡。此外,这项研究强调了Gedang\对全球哺乳动物多样性保护工作的重要性,为有效的野生动物保护策略提供必要的数据。
    The Medog in southeastern Tibet is home to a diverse range of wild animals. However, research on these mammals\' species directories, distribution, and conservation status remains insufficient, despite their crucial role in maintaining ecological balance. The study carried out a camera-trapping survey to assess mammal biodiversity and the significance of mammal protection in their natural habitats in Gedang, Medog. Future directions and application prospects of the study for wildlife conservation in the southeastern Tibetan mountains were also discussed. The survey, spanning from April 2023 to May 2024, with 19,754 camera trap days, revealed 25 mammalian species across five orders and 14 families. Among these, four classified as Endangered, five as Vulnerable, two as Near Threatened on the IUCN Red List, nine were categorized as Critically Endangered or Endangered on the Red List of China\'s Vertebrates, and seven were China\'s national first-class key protected wildlife. The order Carnivora exhibited the highest diversity, comprising 12 species. Furthermore, the study filled the knowledge gap regarding the underrepresentation of Gongshan muntjac Muntiacus gongshanensis in IUCN and provided new insights into the recorded coexistence of the Himalayan red panda Ailurus fulgens and Chinese red panda Ailurus styani along the Yarlung Zangbo River for the first time, and also documented new upper elevation limits for four large to medium-sized species. Regarding the relative abundance indices (RAI) captured by camera traps, the most prevalent species identified was the White-cheeked macaque Macaca leucogenys, followed by the Gongshan muntjac and Himalayan serow Capricornis thar. The monitoring also captured a number of domestic dogs and livestock, as well as human disturbances. These findings underscore the importance of conserving these mammals and emphasize the need for conservation efforts to protect their habitats and reduce human activities that threaten their survival, thereby maintaining the ecological balance of the region. Additionally, the research highlighted Gedang\'s significance to global conservation efforts for mammalian diversity, providing essential data for effective wildlife conservation strategies.
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  • 文章类型: Journal Article
    新烟碱(NEO),最广泛使用的杀虫剂,在环境中无处不在,由于它们的亲水性引起了人们的关注,持久性,潜在的生态风险。作为主要的农药消费国,中国在近地天体污染方面表现出显著的地区差异。这篇评论探讨了NEO分布,来源,以及中国各地的有毒风险。环境样品中确定的主要NEO污染物包括吡虫啉,噻虫嗪,还有啶虫脒.在北方,玉米种植是雨季近地天体的主要来源,而水稻全年在南方占主导地位。在南部地区的水生环境中检测到高浓度的近地天体(130.25ng/L),城市河流教派。(157.66纳克/升),和长江下游断面(58.9纳克/升),表明气候条件和城市污染排放是水污染的重要驱动因素。与其他土壤类型相比,农业土壤中检测到的新烟碱含量更高,南部农业区的浓度(平均27.21ng/g)高于北部地区(平均12.77ng/g)。大气NEO水平较低,最高浓度为1560pg/m3。中国水生环境中新烟碱类农药的总含量主要超过35ng/L的慢性毒性生态阈值,特别是在北京和齐鲁湖流域,它们可能超过200ng/L的急性毒性生态阈值。在未来,努力的重点是新烟碱在中国西南农业发达地区的分布,同时也强调它们在城市绿化和家庭环境中的使用。
    Neonicotinoids (NEOs), the most widely used class of insecticides, are pervasive in the environment, eliciting concerns due to their hydrophilicity, persistence, and potential ecological risks. As the leading pesticide consumer, China shows significant regional disparities in NEO contamination. This review explores NEO distribution, sources, and toxic risks across China. The primary NEO pollutants identified in environmental samples include imidacloprid, thiamethoxam, and acetamiprid. In the north, corn cultivation represents the principal source of NEOs during wet seasons, while rice dominates in the south year-round. The high concentration levels of NEOs have been detected in the aquatic environment in the southern regions (130.25 ng/L), the urban river Sects. (157.66 ng/L), and the downstream sections of the Yangtze River (58.9 ng/L), indicating that climate conditions and urban pollution emissions are important drivers of water pollution. Neonicotinoids were detected at higher levels in agricultural soils compared to other soil types, with southern agricultural areas showing higher concentrations (average 27.21 ng/g) than northern regions (average 12.77 ng/g). Atmospheric NEO levels were lower, with the highest concentration at 1560 pg/m3. The levels of total neonicotinoid pesticides in aquatic environments across China predominantly exceed the chronic toxicity ecological threshold of 35 ng/L, particularly in the regions of Beijing and the Qilu Lake Basin, where they likely exceed the acute toxicity ecological threshold of 200 ng/L. In the future, efforts should focus on neonicotinoid distribution in agriculturally developed regions of Southwest China, while also emphasizing their usage in urban greening and household settings.
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  • 文章类型: Journal Article
    乙基汞(EtHg),类似于甲基汞(MeHg),具有很强的神经毒性和生物蓄积性。尽管最近的研究表明它存在于天然土壤和沉积物中,用于EtHg分析的常见丙基化衍生可能会产生EtHg伪影,可能导致其在环境样本中的高估。此外,EtHg的广泛环境流行仍未得到证实,保持其重要性在很大程度上不确定。这项研究调查了在丙基化衍生化过程中EtHg伪影的形成,用真实样品评估不同提取方法下的伪影形成和回收率,证实了EtHg在中国湿地的广泛存在。在丙基化衍生化过程中明显存在EtHg伪影,并且强烈依赖于衍生化溶液中Hg2(0.1-10ng)的水平(R²=0.99),占Hg2+的1.38-2.14%。CuSO4-HNO3CH2Cl2萃取(有效去除Hg2)与丙基化衍生化相结合,可为土壤/沉积物中的EtHg测量提供出色的回收率(81-86%)和低伪影( Ethylmercury (EtHg), similar to methylmercury (MeHg), is highly neurotoxic and bioaccumulative. Although recent studies suggested its occurrence in natural soils and sediments, the common propylation derivatization for EtHg analysis might generate EtHg artifacts, potentially leading to its overestimation in environmental samples. Furthermore, the extensive environmental prevalence of EtHg remains unverified, keeping its importance largely uncertain. This study investigated the formation of EtHg artifacts during propylation derivatization, evaluating artifacts formation and recoveries under different extraction methods with real samples, and confirmed the widespread occurrence of EtHg in Chinese wetlands. EtHg artifacts were obviously present during the propylation derivatization and strongly dependent on the levels of Hg2+ (0.1-10 ng) in the derivatization solution (R² = 0.99), accounting for 1.38-2.14% of Hg2+. CuSO4-HNO3CH2Cl2 extraction (effectively removing Hg2+) combined with propylation derivatization offers excellent recovery (81-86%) and low artifacts (< LOD: 1.98 × 10-4 ng/g) for EtHg measurement in soils/sediments, with results aligning with those from online solid phase extraction-high performance liquid chromatography-inductively coupled plasma mass spectrometry (R2 = 0.99). Additionally, we observed the occurrence of EtHg in soil and sediment samples across 14 Chinese wetlands, with concentrations varying from 6.08 to 171 pg/g, similar to MeHg concentrations at some sites. EtHg positively correlates with MeHg, total Hg, and total organic carbon across all samples, indicating a possible biological formation. These findings help better understand and predict the prevalence of EtHg in wetlands and its key role in environmental Hg cycle.
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  • 文章类型: Journal Article
    玉米赤霉烯酮,镰刀菌属产生的一种突出的霉菌毒素。,广泛污染谷物和动物饲料。玉米赤霉烯酮的热稳定性对传统的去除方法造成了严重的障碍,这可能会带来新的安全问题,或降低营养质量。相比之下,生物技术提供吸引人的好处,如易于应用和有效,具有低毒性的副产品。因此,本文旨在描述近5年玉米赤霉烯酮在谷物和谷物饲料中的发生情况,概述主要国家关于玉米赤霉烯酮的规章制度,并讨论了控制谷物和谷物基饲料中玉米赤霉烯酮的生物学方法的最新进展。此外,本文还综述了玉米赤霉烯酮去除生物策略在谷物和谷物基饲料中的应用和发展趋势。
    Zearalenone, a prominent mycotoxin produced by Fusarium spp., ubiquitously contaminates cereal grains and animal feedstuffs. The thermal stability of zearalenone creates serious obstacles for traditional removal methods, which may introduce new safety issues, or reducing nutritional quality. In contrast, biological technologies provide appealing benefits such as easy to apply and effective, with low toxicity byproducts. Thus, this review aims to describe the occurrence of zearalenone in cereals and cereal-based feedstuffs in the recent 5 years, outline the rules and regulations regarding zearalenone in the major countries, and discuss the recent developments of biological methods for controlling zearalenone in cereals and cereal-based feedstuffs. In addition, this article also reviews the application and the development trend of biological strategies for removal zearalenone in cereals and cereal-based feedstuffs.
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  • 文章类型: Journal Article
    确定污水处理厂(WWTP)废水中的优先污染物对于优化监测工作至关重要。完善法规,并制定有针对性的缓解策略。尽管污水处理厂废水中存在许多痕量有机污染物,缺乏全面的优先排序方案,阻碍有效控制。本研究筛选了216种微污染物,包括药品,杀虫剂,和工业化学品,在中国46个污水处理厂的废水中检测到了这一点。开发了一种多准则优先排序方法,考虑基于中值浓度和检测频率的暴露潜力,以及由持久性决定的潜在危险,生物蓄积性,体外毒性,和体内毒性。过滤掉低暴露或潜在危险的污染物,并计算了优先指数对其余59种物质进行排名。最优先的15种污染物包括受管制的持久性有机污染物,如全氟辛酸及其替代品,如全氟丁烷磺酸盐,农药转化产品,和新出现的污染物,如双酚A,目前在污水处理厂废水中没有受到监管。这项研究提供了一种系统的方法来识别优先污染物,并为监测,regulation,并控制污水处理厂废水中公认和被忽视的污染物。
    Identifying priority pollutants in wastewater treatment plant (WWTP) effluents is crucial for optimizing monitoring efforts, improving regulations, and developing targeted mitigation strategies. Despite the presence of numerous trace organic pollutants in WWTP effluents, a comprehensive prioritization scheme is lacking, hindering effective control. This study screened 216 micropollutants, including pharmaceuticals, pesticides, and industrial chemicals, which had been detected in effluents from 46 WWTPs across China. A multi-criteria prioritization method was developed, considering exposure potential based on median concentrations and detection frequencies, as well as hazard potential determined by persistence, bioaccumulation, in vitro toxicity, and in vivo toxicity. Pollutants with low exposure or hazard potential were filtered out, and a priority index was calculated to rank the remaining 59 substances. The top 15 priority pollutants included regulated persistent organic pollutants like perfluorooctanoic acid and their alternatives such as perfluorobutane sulfonate, pesticide transformation products, and emerging contaminants such as bisphenol A, which are not currently regulated in WWTP effluents. This study provides a systematic approach to identify priority pollutants and generates a guiding framework for monitoring, regulation, and control of both well-recognized and overlooked contaminants in WWTP effluents.
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  • 文章类型: Journal Article
    背景:描述伊蚊幼虫的时空发生和栖息地特征对于控制伊蚊传播的病毒性疾病至关重要。这项研究评估了南部阿法尔地区伊蚊幼虫的时空丰度和栖息地特征,埃塞俄比亚。
    方法:在AwashSebat调查了未成熟的蚊子,阿沃什·阿尔巴,从2022年5月到2023年4月,南部阿法尔地区的韦勒城镇每月一次。幼虫和p的调查是沿着可用的盛水容器进行的。将收集的幼虫/p饲养成虫,并在形态上按物种/属进行鉴定。还对生境的物理和化学性质进行了表征。
    结果:共收集了9099只伊蚊幼虫/p,其中53.6%(4875)来自AwashSebat,29.5%(2687)来自AwashArba,16.9%(1537)来自Weer。持水轮胎的伊蚊幼虫/p数量最多,其次是储水桶。所有饲养成虫的伊蚊幼虫/p在形态上被鉴定为埃及伊蚊。整体集装箱指数为47.28%,房屋指数18.19%,Breteau指数59.94%,Pupal指数171.94。在Ae的发生中观察到显着的正相关。带保水轮胎的埃及伊蚊幼虫/p(AOR=15.89,CI=3.55-71.09,p<0.001),储水桶(AOR=19.84,CI=4.64-84.89,p<0.001),家庭栖息地(AOR=3.76,CI=1.27-11.12,p=0.017),与Ae呈显著负相关。埃及伊蚊幼虫/p的发生和自来水来源(AOR=0.08,CI=0.02-0.31,p=0.001)。Ae.埃及伊蚊幼虫/p的密度与水的溶解氧(β=0.523,p<0.001)和总硬度(β=0.475,p=0.034)呈正相关。
    结论:不同类型的人工盛水容器对Ae呈阳性。埃及伊蚊幼虫/蛹.Ae.埃及伊蚊幼虫/p在使用过的保水轮胎中很丰富,储水桶,和AwashSebat的水泥罐,阿沃什·阿尔巴,和Werer城镇。这可能会使城镇居民面临感染Ae的高风险。埃及伊蚊传播病毒疾病,如基孔肯雅热和登革热暴发。因此,我们建议将人工盛水容器管理作为控制Ae的策略。埃及伊蚊,从而传播虫媒病毒病。
    BACKGROUND: Describing spatio-temporal occurrence and habitat characteristics of Aedes mosquito larvae is crucial for the control of Aedes borne viral diseases. This study assessed spatio-temporal abundance and habitat characteristics of Aedes larvae in the Southern Afar Region, Ethiopia.
    METHODS: Immature mosquitoes were surveyed in Awash Sebat, Awash Arba, and Werer towns of the Southern Afar Region once per month from May 2022 to April 2023. Larvae and pupae surveys were carried out along the available water-holding containers. The collected larvae/pupae were reared to adults and identified by  species/genus morphologically. The physical and chemical properties of the habitats were also characterized.
    RESULTS: A total of 9099 Aedes larvae/pupae were collected, of which 53.6% (4875) were from Awash Sebat, 29.5% (2687) from Awash Arba and 16.9% (1537) from Werer. Water-holding tyres harboured the highest number of Aedes larvae/pupae followed by water-storage drums. All the Aedes larvae/pupae reared to adults were morphologically identified as Aedes aegypti. The overall Container Index was 47.28%, House Index 18.19%, Breteau Index 59.94% and Pupal Index 171.94. Significant positive relations were observed in the occurrences of Ae. aegypti larvae/pupae with water-holding tyre (AOR = 15.89, CI = 3.55-71.09, p < 0.001), water storage drums (AOR = 19.84, CI = 4.64-84.89, p < 0.001), domestic habitat (AOR = 3.76, CI = 1.27-11.12, p = 0.017), and significant negative relations were observed with Ae. aegypti larvae/pupae occurrence and tap water source (AOR = 0.08, CI = 0.02-0.31, p = 0.001). Ae. aegypti larvae/pupae densities showed positive relations with dissolved oxygen (β = 0.523, p < 0.001) and total hardness (β = 0.475, p = 0.034) of water.
    CONCLUSIONS: Diverse types of artificial water-holding containers were positive for Ae. aegypti larvae/pupae. Ae. aegypti larvae/pupae were abundant in used water-holding tyres, water storage drums, and cement tanks in Awash Sebat, Awash Arba, and Werer towns. This could  put the residents of the towns at high risk of infections with Ae. aegypti transmitted viral diseases such as chikungunya and dengue outbreaks. Thus, we recommend artificial water-holding container management as a strategy to control Ae. aegypti and hence the arboviral diseases transmission.
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