未经证实:肠道微生物群在人类生命的早期阶段发挥着重要作用。我们先前的研究表明,黄疸患儿参与半乳糖代谢的肠道菌群丰度发生了改变,并与血清胆红素水平升高相关。我们进行了本研究,以系统地评估黄疸新生儿胎粪代谢组的变化,并寻找与新生儿黄疸相关的代谢标志物。
未经评估:我们纳入了68例新生儿高胆红素血症,也被称为新生儿黄疸(NJ)和68个匹配的健康对照(HC),在他们出生时收集了胎粪样本,并通过液相色谱-质谱进行代谢组学分析。
UNASSIGNED:肠道代谢产物能够明确区分新生儿黄疸(NJ)和健康对照(HC)组。我们还确定了NJ和HC组之间显著不同的肠道代谢物的组成;这些差异显著的代谢物富含氨基tRNA生物合成;泛酸和辅酶生物合成;和缬氨酸,亮氨酸和异亮氨酸生物合成途径。肠道支链氨基酸(BCAA)水平与血清胆红素水平呈正相关,和基于BCAAs的随机森林分类器模型的接收者工作特性曲线下的面积,脯氨酸,蛋氨酸,苯丙氨酸和总胆红素达到96.9%,显示诊断应用的良好潜力。基于机器学习的因果推断分析揭示了BCAAs对血清总胆红素和NJ的因果效应。
UNASSIGNED:黄疸新生儿肠道代谢产物的改变显示BCAAs升高与血清总胆红素呈正相关。BCAAs脯氨酸,蛋氨酸,苯丙氨酸是NJ的潜在生物标志物。
UNASSIGNED: The gut microbiota plays an important role in the early stages of human life. Our previous study showed that the abundance of intestinal flora involved in galactose metabolism was altered and correlated with increased serum bilirubin levels in children with jaundice. We conducted the present study to systematically evaluate alterations in the meconium metabolome of neonates with jaundice and search for metabolic markers associated with neonatal jaundice.
UNASSIGNED: We included 68 neonates with neonatal hyperbilirubinemia, also known as neonatal jaundice (NJ) and 68 matched healthy controls (HC), collected meconium samples from them at birth, and performed metabolomic analysis via liquid chromatography-mass spectrometry.
UNASSIGNED: Gut metabolites enabled clearly distinguishing the neonatal jaundice (NJ) and healthy control (HC) groups. We also identified the compositions of the gut metabolites that differed significantly between the NJ and HC groups; these differentially significant metabolites were enriched in aminyl tRNA biosynthesis; pantothenic acid and coenzyme biosynthesis; and the valine, leucine and isoleucine biosynthesis pathways. Gut branched-chain amino acid (BCAA) levels were positively correlated with serum bilirubin levels, and the area under the receiver operating characteristic curve of the random forest classifier model based on BCAAs, proline, methionine, phenylalanine and total bilirubin reached 96.9%, showing good potential for diagnostic applications. Machine learning-based causal inference analysis revealed the causal effect of BCAAs on serum total bilirubin and NJ.
UNASSIGNED: Altered gut metabolites in neonates with jaundice showed that increased BCAAs and total serum bilirubin were positively correlated. BCAAs proline, methionine, phenylalanine are potential biomarkers of NJ.