未经批准:骨生成和血管生成对骨折愈合很重要。Irisin是一种肌肉衍生的单核因子,与骨形成有关。
未经评估:为了证明irisin对骨折愈合的影响,闭合性股骨中段骨折在8周龄C57BL/6小鼠中产生.手术后每隔一天腹膜内注射Irisin,骨折愈合通过X线片进行评估.使用显微计算机断层扫描评估骨折骨痂的骨形态。通过三点弯曲测试评估来自每组的小鼠的股骨。通过定量实时聚合酶链反应(qRT-PCR)评估irisin对间充质干细胞成骨分化的影响。碱性磷酸酶染色和茜素红染色。通过qRT-PCR评估人脐静脉内皮细胞(HUVECs)的血管生成,迁移测试,和试管形成测定。
未经证实:愈伤组织形成增加,与对照组相比,irisin治疗组观察到矿化和更坚韧的骨折愈合,表明由于Irisin治疗,骨折骨痂愈合更好。在irisin治疗组中,愈伤组织的血管表面和血管体积分数也增加。在irisin治疗组中,愈伤组织中BMP2,CD31和VEGF的表达增强。在小鼠骨髓间充质干细胞中,irisin促进ALP表达和矿化,并增加了成骨基因的表达,包括OSX,Runx2,OPG,ALP,OCN和BMP2。Irisin还促进HUVEC迁移和管形成。血管生成基因的表达,包括ANGPT1,ANGPT2,VEGFb,HUVEC中CD31、FGF2和PDGFRB被irisin增加。
UNASSIGNED:所有结果表明irisin可以通过成骨和血管生成促进骨折愈合。这些发现有助于理解骨折愈合过程中的肌肉-骨骼相互作用。
未经证实:Irisin是骨骼肌分泌的最重要的单核因子之一。研究发现,irisin通过影响骨细胞和成骨细胞而具有合成代谢作用。根据我们的研究,irisin可以通过增加骨量和血管形成来促进骨折愈合,这提供了irisin促进骨折愈合和改善临床结果的潜在用途。
UNASSIGNED: Osteogenesis and angiogenesis are important for bone fracture healing. Irisin is a muscle-derived monokine that is associated with bone formation.
UNASSIGNED: To demonstrate the effect of irisin on bone fracture healing, closed mid-diaphyseal femur fractures were produced in 8-week-old C57BL/6 mice. Irisin was administrated intraperitoneally every other day after surgery, fracture healing was assessed by using X-rays. Bone morphometry of the fracture callus were assessed by using micro-computed tomography. Femurs of mice from each group were assessed by the three-point bending testing. Effect of irisin on osteogenic differentiation in mesenchymal stem cells in vitro was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR), alkaline phosphatase staining and alizarin red staining. Angiogenesis of human umbilical vein endothelial cells (HUVECs) were evaluated by qRT-PCR, migration tests, and tube formation assays.
UNASSIGNED: Increased callus formation, mineralization and tougher fracture healing were observed in the irisin-treated group than in the control group, indicating the better fracture callus healing due to Irisin treatment. The vessel surface and vessel volume fraction of the callus also increased in the irisin-treated group. The expression of BMP2, CD31, and VEGF in callus were enhanced in the irisin-treated group. In mouse bone mesenchymal stem cells, irisin promoted ALP expression and mineralization, and increased the expression of osteogenic genes, including OSX, Runx2, OPG, ALP, OCN and BMP2. Irisin also promoted HUVEC migration and tube formation. Expression of angiogenic genes, including ANGPT1, ANGPT2, VEGFb, CD31, FGF2, and PDGFRB in HUVECs were increased by irisin.
UNASSIGNED: All the results indicate irisin can promote fracture healing through osteogenesis and angiogenesis. These findings help in the understanding of muscle-bone interactions during fracture healing.
UNASSIGNED: Irisin was one of the most important monokine secreted by skeletal muscle. Studies have found that irisin have anabolic effect one bone remodeling through affecting osteocyte and osteoblast. Based on our study, irisin could promote bone fracture healing by increasing bone mass and vascularization, which provide a potential usage of irisin to promote fracture healing and improve clinical outcomes.