未经证实:血吸虫病是一种寄生虫感染,影响全球超过2亿人。血吸虫卵,但不是成虫,主要负责血吸虫病在肝脏的特定发病率。目前尚不清楚曼氏链球菌卵是否消耗宿主代谢产物,以及这如何影响宿主父母。
UNASSIGNED:通过基质辅助激光解吸/电离质谱成像分析了代谢重编程,液相色谱与高分辨率质谱,代谢物定量,共聚焦激光扫描显微镜,活细胞成像,实时定量PCR,西方印迹,评估DNA损伤,仓鼠模型的免疫组织学和人类细胞系的功能实验。主要结果在人体活检中得到验证。
未经证实:曼氏链球菌感染会引起中性脂质和糖原的肝衰竭。此外,不同的脂质种类的分布和限速代谢酶的调节在曼氏链球菌感染的动物的肝脏中被破坏。值得注意的是,鸡蛋动员,合并,并储存宿主脂质,而相关的代谢重编程导致氧化应激诱导的肝细胞DNA损伤。活性氧物质清除剂的施用改善了这些有害作用。
UNASSIGNED:我们的研究结果表明,曼氏链球菌卵通过可溶性因子完全重新编程脂质和碳水化合物代谢,导致宿主薄壁组织中氧化应激诱导的细胞损伤。
UNASSIGNED:作者证明了寄生虫S.mansoni的可溶性蛋制品诱导肝细胞重编程,导致代谢衰竭和强烈的氧化还原失衡。值得注意的是,鸡蛋动员,合并,并储存宿主脂质,而代谢重编程导致氧化应激诱导的肝细胞DNA损伤,独立于宿主的免疫反应。曼氏虫卵通过肝细胞和肠细胞的代谢重编程利用宿主环境。通过诱导DNA损伤,这种被忽视的热带病可能会促进肝细胞损伤,从而影响国际卫生工作。
UNASSIGNED: Schistosomiasis is a parasitic infection which affects more than 200 million people globally. Schistosome eggs, but not the adult worms, are mainly responsible for schistosomiasis-specific morbidity in the liver. It is unclear if S. mansoni eggs consume host metabolites, and how this compromises the host parenchyma.
UNASSIGNED: Metabolic reprogramming was analyzed by matrix-assisted laser desorption/ionization mass spectrometry imaging, liquid chromatography with high-resolution mass spectrometry, metabolite quantification, confocal laser scanning microscopy, live cell imaging, quantitative real-time PCR, western blotting, assessment of DNA damage, and immunohistology in hamster models and functional experiments in human cell lines. Major results were validated in human biopsies.
UNASSIGNED: The infection with S. mansoni provokes hepatic exhaustion of neutral lipids and glycogen. Furthermore, the distribution of distinct lipid species and the regulation of rate-limiting metabolic enzymes is disrupted in the liver of S. mansoni infected animals. Notably, eggs mobilize, incorporate, and store host lipids, while the associated metabolic reprogramming causes oxidative stress-induced DNA damage in hepatocytes. Administration of reactive oxygen species scavengers ameliorates these deleterious effects.
UNASSIGNED: Our findings indicate that S. mansoni eggs completely reprogram lipid and carbohydrate metabolism via soluble factors, which results in oxidative stress-induced cell damage in the host parenchyma.
UNASSIGNED: The authors demonstrate that soluble egg products of the parasite S. mansoni induce hepatocellular reprogramming, causing metabolic exhaustion and a strong redox imbalance. Notably, eggs mobilize, incorporate, and store host lipids, while the metabolic reprogramming causes oxidative stress-induced DNA damage in hepatocytes, independent of the host\'s immune response. S. mansoni eggs take advantage of the host environment through metabolic reprogramming of hepatocytes and enterocytes. By inducing DNA damage, this neglected tropical disease might promote hepatocellular damage and thus influence international health efforts.