背景:在当前由新型冠状病毒引起的大流行的背景下,分子检测不仅限于临床实验室,同时也面临着复杂多变的实时检测领域的挑战。在食品冷链包装和运输过程中检测到一系列新型冠状病毒事件,分子诊断在食品加工中的应用,包装,交通运输,和其他联系紧急。迫切需要一种能够适应食品安全多样性和复杂性的快速检测技术。
UNASSIGNED:这篇综述介绍了一种新的分子诊断技术-基于CRISPR-Cas12a的生物传感器分析技术。系统地阐明其发展过程和检测原理。它总结并系统地组织了它在病毒中的应用,食源性致病菌,小分子检测,等。在过去的四年里,为食品检测提供了全新的全面解决方案。最后,本文提出了食品安全面临的挑战和前景。
结论:新型冠状病毒危害渗透到食品工业的每一步,从加工到包装到运输。基于CRISPR-Cas12a的生物传感器分析技术在感染性病原体的定性和定量分析中具有巨大的潜力。CRISPR-Cas12a可以有效地识别特定核酸靶标的存在和序列的微小变化,这对于核酸鉴定和病原体检测尤为重要。此外,CRISPR-Cas12a方法可以在几天内调整和重新配置以检测其他病毒,为食品安全领域的核酸诊断提供设备。未来的工作将集中在用于多重检测的便携式微流体设备的开发上。Shaoetal.采用物理分离方法分离不同微流体通道中的Cas蛋白,实现多重检测,每个通道通过添加具有不同间隔序列的crRNA同时检测不同的靶标。虽然CRISPR-Cas12a技术在检测方面具有突出的优势,从新兴技术向实际应用的转变存在几个技术壁垒。新开发的基于CRISPR-Cas12a的应用和方法促进了众多诊断和检测解决方案的发展,并且在医学诊断方面有很大的潜力,环境监测,尤其是食品检测。
BACKGROUND: In the context of the current pandemic caused by the novel coronavirus, molecular detection is not limited to the clinical laboratory, but also faces the challenge of the complex and variable real-time detection fields. A series of novel coronavirus events were detected in the process of food cold chain packaging and transportation, making the application of molecular diagnosis in food processing, packaging, transportation, and other links urgent. There is an urgent need for a rapid detection technology that can adapt to the diversity and complexity of food safety.
UNASSIGNED: This review introduces a new molecular diagnostic technology-biosensor analysis technology based on CRISPR-Cas12a. Systematic clarification of its development process and detection principles. It summarizes and systematically organizes its applications in viruses, food-borne pathogenic bacteria, small molecule detection, etc. In the past four years, which provides a brand-new and comprehensive solution for food detection. Finally, this article puts forward the challenges and the prospects for food safety.
CONCLUSIONS: The novel coronavirus hazards infiltrated every step of the food industry, from processing to packaging to transportation. The biosensor analytical technology based on CRISPR-Cas12a has great potential in the qualitative and quantitative analysis of infectious pathogens. CRISPR-Cas12a can effectively identify the presence of the specific nucleic acid targets and the small changes in sequences, which is particularly important for nucleic acid identification and pathogen detection. In addition, the CRISPR-Cas12a method can be adjusted and reconfigured within days to detect other viruses, providing equipment for nucleic acid diagnostics in the field of food safety. The future work will focus on the development of portable microfluidic devices for multiple detection. Shao et al. employed physical separation methods to separate Cas proteins in different microfluidic channels to achieve multiple detection, and each channel simultaneously detected different targets by adding crRNA with different spacer sequences. Although CRISPR-Cas12a technology has outstanding advantages in detection, there are several technical barriers in the transformation from emerging technologies to practical applications. The newly developed CRISPR-Cas12a-based applications and methods promote the development of numerous diagnostic and detection solutions, and have great potential in medical diagnosis, environmental monitoring, and especially food detection.