一种新型革兰氏染色阴性菌株,指定JM10B15T,从江门市收集的凡纳滨对虾池塘水中分离出来,广东省,南公关中国。该菌株的细胞是有氧的,杆状,并通过侧面鞭毛运动。JM10B15T可以在15-40°C生长,pH6.0-9.5,在0-3.0%NaCl中,在25-35°C下最佳生长,pH7.5-8.5,在0%NaCl中,分别。此外,该菌株在Reasoner的2A琼脂上生长良好,但在营养肉汤琼脂或Luria-Bertani琼脂上生长良好。JM10B15T是一种能够去除亚硝酸盐和硝酸盐的反硝化细菌,和三个关键的功能基因,美国国家航空航天局,nirs,和nosz,在它的基因组中被鉴定出来。基于16SrRNA基因和基因组序列的系统发育分析结果表明,JM10B15T属于Gemmobacter属。JM10B15T显示出最高的16SrRNA序列相似性,与绿藻GemmobacterYJ-T1-11T(98.8%),其次是水杆菌IFAM1031T(98.6%)和蛇形杆菌HB-1T(98.1%)。JM10B15T与Gemobacter属其他类型菌株的平均核苷酸同一性和数字DNA-DNA杂交值分别为78.1-82.1%和18.4-22.1%,分别。菌株JM10B15T的主要脂肪酸为特征8(C18:1ω6c和/或C18:1ω7c)和C18:1ω7c11-甲基。此外,该新菌株的主要呼吸醌是Q-10,主要的极性脂质是磷脂酰甘油,磷脂酰乙醇胺,四种身份不明的磷脂,三种身份不明的脂质,还有一种身份不明的氨基磷脂.系统发育分析的结果,基因组,生理,和生化特征表明JM10B15T代表了Gemmobacter属的一种新物种,名称为Gemmobacterdenitrificanssp。11月。是提议的。菌株类型为JM10B15T(=GDMCC1.4148T=KCTC8140T)。
A novel Gram-stain-negative strain, designated JM10B15T, was isolated from pond water for Litopenaeus vannamei collected from Jiangmen City, Guangdong province, south PR China. Cells of the strain were aerobic, rod-shaped, and motile by lateral flagella. JM10B15T could grow at 15-40 °C, pH 6.0-9.5, and in 0-3.0 % NaCl, with optimal growth at 25-35 °C, pH 7.5-8.5, and in 0 % NaCl, respectively. Furthermore, this strain grew well on Reasoner\'s 2A agar but not on nutrient broth agar or Luria-Bertani agar. JM10B15T was a denitrifying bacterium capable of removing
nitrites and nitrates, and three key functional genes, nasA, nirS, and nosZ, were identified in its genome. The results of phylogenetic analyses based on the 16S rRNA gene and genome sequences indicated that JM10B15T belonged to the genus Gemmobacter. JM10B15T showed the highest 16S rRNA sequence similarity to Gemmobacter lutimaris YJ-T1-11T (98.8 %), followed by Gemmobacter aquatilis IFAM 1031T (98.6 %) and Gemmobacter serpentinus HB-1T (98.1 %). The average nucleotide identity and digital DNA-DNA hybridization values between JM10B15T and the other type strains of genus Gemmobacter were 78.1-82.1 % and 18.4-22.1 %, respectively. The major fatty acids of strain JM10B15T were summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C18 : 1 ω7c 11-methyl. In addition, the major respiratory quinone of this novel strain was Q-10, and the predominant polar lipids were phosphatidylglycerol, phosphatidylethanolamine, four unidentified phospholipids, three unidentified lipids, and an unidentified aminophospholipid. Results of analyses of the phylogenetic, genomic, physiological, and biochemical characteristics indicated that JM10B15T represents a novel species of the genus Gemmobacter, for which the name Gemmobacter denitrificans sp. nov. is proposed. The type strain is JM10B15T (=GDMCC 1.4148T=KCTC 8140T).