伤口愈合一直是医生和初级卫生保健系统的严重问题。此外,脂肪干细胞来源的外泌体已被证明在组织修复和再生中起着积极有效的作用。对这些临床前研究进行了系统评价,以评估脂肪干细胞来源的外泌体(ADSC-Exos)在治疗伤口中的功效。本文旨在研究ADSC-Exos治疗动物皮肤伤口的有效性,并对一般伤口和糖尿病溃疡伤口的外泌体在动物体外模型中的作用进行荟萃分析,为临床翻译提供理论依据。
■通过搜索PubMed,确定了总共19项针对356只动物的研究,科克伦,MEDLINE完成,WebofScience,CNKI和万方数据库从成立到2022年11月15日。没有语言或时间限制。Stata17用于所有数据分析。
■荟萃分析表明,ADSC-Exo疗法可显著提高对照组的伤口愈合率,除了第7天的糖尿病组。一般伤口的第7天[标准平均差(SMD)2.87,95%置信区间(CI)1.91-3.83]和第14天(SMD2.89,95CI1.47-4.30)。糖尿病伤口的第14天(SMD3.43,95CI1.28-5.58)。其他成果,比如血管密度,胶原沉积和伤口再上皮化,随着ADSC-Exos的管理而改善。
■一项荟萃分析表明,ADSC-Exo疗法应用于全身和糖尿病伤口可促进新生血管形成,改善上皮形成和胶原纤维沉积,促进愈合,减少疤痕的形成。ADSC-Exos在临床前研究和临床领域具有广泛的潜力。
UNASSIGNED: Wound healing has always been a serious issue for doctors and primary health care systems. In addition, adipose stem cell-derived exosomes have been proven to play a positive and effective role in tissue repair and regeneration. A systematic review of these preclinical studies was performed to assess the efficacy of adipose stem cell-derived exosomes (ADSC-Exos) in treating wounds. This article aimed to study the effectiveness of ADSC-Exos for the treatment of animal skin wounds and includes a meta-analysis of exosomes from general wounds and diabetic ulcer wounds in in vitro models of animals to provide a theoretical basis for clinical translation.
UNASSIGNED: A total of 19 studies with 356 animals were identified by searching the PubMed, Cochrane, MEDLINE Complete, Web of Science, CNKI and Wanfang databases from inception to 15 November 2022. No language or time restrictions were applied. Stata17 was used for all the data analyses.
UNASSIGNED: The meta-analysis showed that ADSC-Exo therapy significantly improved the wound healing rate in the control group, except in the diabetes group on day 7. Day 7 of general wounds [standard mean difference (SMD) 2.87, 95% confidence interval (CI) 1.91-3.83)] and day 14 (SMD 2.89, 95%CI 1.47-4.30). Day 14 (SMD 3.43, 95%CI 1.28-5.58) of diabetic wounds. Other outcomes, such as blood vessel density, collagen deposition and wound re-epithelization, improved with the administration of ADSC-Exos.
UNASSIGNED: A meta-analysis showed that ADSC-Exo therapy applied to general and diabetic wounds can promote
neovascularization, improve epithelization and collagen fiber deposition, promote healing, and reduce scar formation. ADSC-Exos have broad potential in preclinical research and clinical fields.