本文介绍了Brazzein和Monellin的综合毒性评估结果,酵母生产的重组甜味蛋白。过量食用糖是世界上主要的饮食和营养问题之一,导致肥胖等健康并发症,高血压,和心血管疾病。虽然人工小分子甜味剂广泛替代食品中的糖,它们的安全性和长期健康影响仍有争议。许多甜味蛋白质,包括Thaumatin,Miraculin,五肽,Curculin,马宾林,Brazzein,莫内林在热带植物中被发现。这些蛋白质,比如Brazzein和Monellin,比蔗糖甜数千倍。多个报道已经提出了重组甜味蛋白的制备。要在食品工业中引入和应用甜味蛋白,必须对其毒性和安全性进行全面全面的评估。我们通过实验评估了急性,亚慢性,和慢性毒性作用,以及重组Brazzein和Monellin的致敏和诱变特性。我们的研究是在三种哺乳动物物种(小鼠,老鼠,和豚鼠)。动物生理评估,生物化学,血液学,形态学,和行为指数使我们能够断言莫内林和布拉泽因对哺乳动物生物是安全无毒的,这为它们在食品工业中作为糖替代品的应用提供了巨大的机会。
This article presents the results of a comprehensive toxicity assessment of brazzein and monellin, yeast-produced recombinant sweet-tasting proteins. Excessive sugar consumption is one of the leading dietary and nutritional problems in the world, resulting in health complications such as obesity, high blood pressure, and cardiovascular disease. Although artificial small-molecule sweeteners widely replace sugar in food, their safety and long-term health effects remain debatable. Many sweet-tasting proteins, including thaumatin, miraculin, pentadin, curculin, mabinlin, brazzein, and monellin have been found in tropical plants. These proteins, such as brazzein and monellin, are thousands-fold sweeter than sucrose. Multiple reports have presented preparations of recombinant sweet-tasting proteins. A thorough and comprehensive assessment of their toxicity and safety is necessary to introduce and apply sweet-tasting proteins in the food industry. We experimentally assessed acute, subchronic, and chronic toxicity effects, as well as allergenic and mutagenic properties of recombinant brazzein and monellin. Our study was performed on three mammalian species (mice, rats, and guinea pigs). Assessment of animals\' physiological, biochemical, hematological, morphological, and behavioral indices allows us to assert that monellin and brazzein are safe and nontoxic for the mammalian organism, which opens vast opportunities for their application in the food industry as sugar alternatives.