气候变化和人类活动导致盐度水平和铬(Cr)毒性的增加。在植物中改变这些应激源的一种有希望的方法是使用有效的纳米颗粒(NPs)。虽然二氧化钛纳米颗粒(TiO2NP)和羟基磷灰石(HAPNP)已被证明可以通过增强抗氧化能力来提高植物对非生物胁迫的耐受性,脂质过氧化,和次生代谢产物,尚不清楚这两种化合物在存在盐和Cr毒性的情况下如何协同工作。当前研究的目的是确定叶面施用的TiO2NPs(15mgL-1)和HAPNPs(250mgL-1)分别和组合对生长的影响,叶绿素(Chl),含水量,脂质过氧化,抗氧化能力,酚类物质含量,和盐度(100mMNaCl)和Cr毒性(100mgkg-1土壤)下的加拿大一枝黄花精油(EOs)。通过降低植物重量,盐度比Cr更有害,Chla+b,相对含水量(RWC),EO产率,增加丙二醛(MDA),电解液泄漏(EL),超氧化物歧化酶(SOD)活性,和过氧化氢酶(CAT)活性。TiO2和HAPNP的共同应用被证明是更成功的。这证明了增加的枝条重量(36%),根重(29%),Chla+b(23%),RWC(15%),总酚含量(TPC,34%),总黄酮含量(TFC,28%),和EO收率(56%),但MDA降低(21%),EL(11%),盐暴露植物中的SOD(22%)和CAT活性(38%)。该研究证明了共同应用这些NP以通过提高酚类化合物和EO产量作为关键结果来修饰非生物胁迫的有效策略。
Climate change and human activity have led to an increase in salinity levels and the toxicity of chromium (Cr). One promising approach to modifying these stressors in plants is to use effective nanoparticles (NPs). While titanium dioxide nanoparticles (TiO2 NPs) and hydroxyapatite (HAP NPs) have been demonstrated to increase plant tolerance to abiotic stress by enhancing antioxidant capacity, lipid peroxidation, and secondary metabolites, it is unknown how these two compounds can work together in situations when salt and Cr toxicity are present. The objective of the current study was to determine the effects of foliar-applied TiO2 NPs (15 mg L-1) and HAP NPs (250 mg L-1) separately and in combination on growth, chlorophyll (Chl), water content, lipid peroxidation, antioxidant capacity, phenolic content, and essential oils (EOs) of Solidago canadensis L. under salinity (100 mM NaCl) and Cr toxicity (100 mg kg-1 soil). Salinity was more deleterious than Cr by decreasing plant weight, Chl a + b, relative water content (RWC), EO yield, and increasing malondialdehyde (MDA), electrolyte leakage (EL), superoxide dismutase (SOD) activity, and catalase (CAT) activity. The co-application of TiO2 and HAP NPs proved to be more successful. This was evidenced by the increased shoot weight (36%), root weight (29%), Chl a + b (23%), RWC (15%), total phenolic content (TPC, 34%), total flavonoid content (TFC, 28%), and EO yield (56%), but decreased MDA (21%), EL (11%), SOD (22%) and CAT activity (38%) in salt-exposed plants. The study demonstrated the effective strategy of co-applying these NPs to modify abiotic stress by enhancing phenolic compounds and EO yield as key results.