■骨组织工程(BTE)是一种有前途的替代自体骨移植的临床治疗骨缺损,无机/有机复合水凝胶作为BTE支架是当前研究的热点。纳米羟基磷灰石/明胶甲基丙烯酸酯/氧化海藻酸钠(nHAP/GelMA/OSA)的构建,缩写为HGO,复合水凝胶加载骨形态发生蛋白7(BMP7)将提供一个合适的三维微环境,以促进细胞聚集,扩散,和差异化,从而促进骨修复和再生。
■通过将GelMA和OSA组合制备了双重交联的水凝胶,而HGO水凝胶通过掺入不同量的nHAP来配制。对水凝胶进行物理和化学表征,然后评估其生物相容性。BMP7-HGO(BHGO)水凝胶通过将合适浓度的BMP7掺入到HGO水凝胶中来制备。然后通过体外实验并使用大鼠股骨缺损模型验证BHGO水凝胶的成骨潜力。
■nHAP的添加显着改善了水凝胶的物理性质,和10%nHAP的复合水凝胶在所有组中表现出最佳的整体性能。选定浓度的HGO水凝胶用作BMP7负载的载体,并在体内和体外评估其成骨潜力。与空白对照中观察到的结果相比,BHGO水凝胶显示出优异的体外成骨诱导和体内修复骨组织的潜力。BMP7和HGO组。
■使用含有10%HGO的水凝胶似乎有望用于骨组织工程支架,特别是当负载BMP7以增强其成骨潜力时。然而,需要进一步的调查来优化GelMA,OSA,和nHAP比率,随着BMP7的浓度,最大限度地发挥成骨潜力。
UNASSIGNED: Bone tissue engineering (BTE) is a promising alternative to autologous bone grafting for the clinical treatment of bone defects, and inorganic/organic composite hydrogels as BTE scaffolds are a hot spot in current research. The construction of nano-hydroxyapatite/gelatin methacrylate/oxidized sodium alginate (nHAP/GelMA/OSA), abbreviated as HGO, composite hydrogels loaded with bone morphogenetic protein 7 (BMP7) will provide a suitable 3D microenvironment to promote cell aggregation, proliferation, and differentiation, thus facilitating bone repair and regeneration.
UNASSIGNED: Dually-crosslinked hydrogels were fabricated by combining GelMA and OSA, while HGO hydrogels were formulated by incorporating varying amounts of nHAP. The hydrogels were physically and chemically characterized followed by the assessment of their biocompatibility. BMP7-HGO (BHGO) hydrogels were fabricated by incorporating suitable concentrations of BMP7 into HGO hydrogels. The osteogenic potential of BHGO hydrogels was then validated through in vitro experiments and using rat femoral defect models.
UNASSIGNED: The addition of nHAP significantly improved the physical properties of the hydrogel, and the composite hydrogel with 10% nHAP demonstrated the best overall performance among all groups. The selected concentration of HGO hydrogel served as a carrier for BMP7 loading and was evaluated for its osteogenic potential both in vivo and in vitro. The BHGO hydrogel demonstrated superior in vitro osteogenic induction and in vivo potential for repairing bone tissue compared to the outcomes observed in the blank control, BMP7, and HGO groups.
UNASSIGNED: Using hydrogel containing 10% HGO appears promising for bone tissue engineering scaffolds, especially when loaded with BMP7 to boost its osteogenic potential. However, further investigation is needed to optimize the GelMA, OSA, and nHAP ratios, along with the BMP7 concentration, to maximize the osteogenic potential.