PDF(Pubmed)
Abstract：
The aim of this study is to introduce a dental capping agent for the treatment of pulp inflammation (pulpitis). Nanohydroxyapatite with Elaeagnus angustifolia L. extract (nHAEA) loaded with metronidazole (nHAEA@MTZ) was synthesized and evaluated using a lipopolysaccharide (LPS) in vitro model of pulpitis. nHAEA was synthesized through sol-gel method and analyzed using Scanning Electron Microscopy, Transmission Electron Microscopy, and Brunauer Emmett Teller. Inflammation in human dental pulp stem cells (HDPSCs) induced by LPS. A scratch test assessed cell migration, RT PCR measured cytokines levels, and Alizarin red staining quantified odontogenesis. The nHAEA nanorods were 17-23 nm wide and 93-146 nm length, with an average pore diameter of 27/312 nm, and a surface area of 210.89 m2/g. MTZ loading content with controlled release, suggesting suitability for therapeutic applications. nHAEA@MTZ did not affect the odontogenic abilities of HDPSCs more than nHAEA. However, it was observed that nHAEA@MTZ demonstrated a more pronounced anti-inflammatory effect. HDPSCs treated with nanoparticles exhibited improved migration compared to other groups. These findings demonstrated that nHAEA@MTZ could be an effective material for pulp capping and may be more effective than nHAEA in reducing inflammation and activating HDPSCs to enhance pulp repair after pulp damage.
摘要：
这项研究的目的是介绍一种用于治疗牙髓炎症（牙髓炎）的牙齿盖帽剂。合成了负载甲硝唑（nHAEA@MTZ）的带有沙枣提取物（nHAEA）的纳米羟基磷灰石，并使用脂多糖（LPS）的牙髓炎体外模型进行了评估。nHAEA通过溶胶-凝胶法合成，并使用扫描电子显微镜进行分析，透射电子显微镜,还有BrunauerEmmettTeller.LPS诱导的人牙髓干细胞（HDPSC）的炎症。划痕测试评估细胞迁移，RTPCR测量细胞因子水平，和茜素红染色定量牙本质形成。nHAEA纳米棒的宽度为17-23nm，长度为93-146nm，平均孔径为27/312nm，表面积为210.89m2/g。具有受控释放的MTZ装载内容物，建议适合治疗应用。nHAEA@MTZ对HDPSCs牙源性能力的影响不超过nHAEA。然而,观察到nHAEA@MTZ表现出更明显的抗炎作用。与其他组相比,用纳米颗粒处理的HDPSC表现出改善的迀移。这些发现表明，nHAEA@MTZ可能是一种有效的盖髓材料，并且可能比nHAEA更有效地减少炎症和激活HDPSC以增强牙髓损伤后的牙髓修复。
