BACKGROUND: Colorectal cancer is a common disease worldwide with non-specific symptoms such as blood in the stool, bowel movements, weight loss and fatigue. Chemotherapy drugs can cause side effects such as nausea, vomiting and a weakened immune system. The use of antioxidants such as hesperidin could reduce the side effects, but its low bioavailability is a major problem. In this research, we aimed to explore the drug delivery and efficiency of this antioxidant on the HCT116 colorectal cancer cell line by loading hesperidin into PLGA nanoparticles.
METHODS: Hesperidin loaded PLGA nanoparticles were produced by single emulsion evaporation method. The physicochemical properties of the synthesized hesperidin-loaded nanoparticles were determined using SEM, AFM, FT-IR, DLS and UV-Vis. Subsequently, the effect of the PLGA loaded hesperidin nanoparticles on the HCT116 cell line after 48 h was investigated by MTT assay at three different concentrations of the nanoparticles.
RESULTS: The study showed that 90% of hesperidin were loaded in PLGA nanoparticles by UV-Vis spectrophotometry and FT-IR spectrum. The nanoparticles were found to be spherical and uniform with a hydrodynamic diameter of 76.2 nm in water. The release rate of the drug was about 93% after 144 h. The lowest percentage of cell viability of cancer cells was observed at a concentration of 10 µg/ml of PLGA nanoparticles loaded with hesperidin.
CONCLUSIONS: The results indicate that PLGA nanoparticles loaded with hesperidin effectively reduce the survival rate of HCT116 colorectal cancer cells. However, further studies are needed to determine the appropriate therapeutic dosage and to conduct animal and clinical studies.

Breast phyllodes tumor (PT) is a rare fibroepithelial neoplasm with potential malignant behavior. Long non-coding RNAs (lncRNAs) play multifaceted roles in various cancers, but their involvement in breast PT remains largely unexplored. In this study, microarray was leveraged for the first time to investigate the role of lncRNA in PT. We identified lncRNA ZFPM2-AS1 was significantly upregulated in malignant PT, and its overexpression endowed PT with high tumor grade and adverse prognosis. Furthermore, we elucidated that ZFPM2-AS1 promotes the proliferation, migration, and invasion of malignant PT in vitro. Targeting ZFPM2-AS1 through nanomaterial-mediated siRNA delivery in patient-derived xenograft (PDX) model could effectively inhibit tumor progression in vivo. Mechanistically, our findings showed that ZFPM2-AS1 is competitively bound to CDC42, inhibiting ACK1 and STAT1 activation, thereby launching the transcription of TNFRSF19. In conclusion, our study provides evidence that ZFPM2-AS1 plays a pivotal role in the pathogenesis of breast PT, and suggests that ZFPM2-AS1 could serve as a prognostic indicator for patients with PT as well as a promising novel therapeutic target.

This review describes the formation of a protein corona (or its absence) on different classes of nanoparticles, its basic principles, and its consequences for nanomedicine. For this purpose, it describes general concepts to control (guide/minimize) the interaction between artificial nanoparticles and plasma proteins to reduce protein corona formation. Thereafter, methods for the qualitative or quantitative determination of protein corona formation are presented, as well as the properties of nanoparticle surfaces, which are relevant for protein corona prevention (or formation). Thereby especially the role of grafting density of hydrophilic polymers on the surface of the nanoparticle is discussed to prevent the formation of a protein corona. In this context also the potential of detergents (surfactants) for a temporary modification as well as grafting-to and grafting-from approaches for a permanent modification of the surface are discussed. The review concludes by highlighting several promising avenues. This includes (i) the use of nanoparticles without protein corona for active targeting, (ii) the use of synthetic nanoparticles without protein corona formation to address the immune system, (iii) the recollection of nanoparticles with a defined protein corona after in vivo application to sample the blood proteome and (iv) further concepts to reduce protein corona formation.

BACKGROUND: Biosynthesis of metallic nanoparticles using microorganisms are a fabulous and emerging eco-friendly science with well-defined sizes, shapes and controlled monodispersity. Copper nanoparticles, among other metal particles, have sparked increased attention due to their applications in electronics, optics, catalysis, and antimicrobial agents.
RESULTS: This investigation explains the biosynthesis and characterization of copper nanoparticles from soil strains, Niallia circulans G9 and Paenibacillus sp. S4c by an eco-friendly method. The maximum reduction of copper ions and maximum synthesis CuNPs was provided by these strains. Biogenic formation of CuNPs have been characterized by UV-visible absorption spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, X-ray analysis and transmission electron microscopy analysis. Using UV-visible spectrum scanning, the synthesised CuNPs\' SPR spectra showed maximum absorption peaks at λ304&308 nm. TEM investigation of the produced CuNPs revealed the development of spherical/hexagonal nanoparticles with a size range of 13-100 nm by the G9 strain and spherical nanoparticles with a size range of 5-40 nm by the S4c strain. Functional groups and chemical composition of CuONPs were also confirmed. The antimicrobial activity of the biosynthesized CuNPs were investigated against some human pathogens. CuNPs produced from the G9 strain had the highest activity against Candida albicans ATCC 10,231 and the lowest against Pseudomonas aeruginosa ATCC 9027. CuNPs from the S4c strain demonstrated the highest activity against Escherichia coli ATCC 10,231 and the lowest activity against Klebsiella pneumonia ATCC 13,883.
CONCLUSIONS: The present work focused on increasing the CuNPs production by two isolates, Niallia circulans G9 and Paenibacillus sp. S4c, which were then characterized alongside. The used analytics and chemical composition techniques validated the existence of CuONPs in the G9 and S4c biosynthesized nano cupper. CuNPs of S4c are smaller and have a more varied shape than those of G9 strain, according to TEM images. In terms of antibacterial activity, the biosynthesized CuNPs from G9 and S4c were found to be more effective against Candida albicans ATCC 10,231 and E. coli ATCC 10,231, respectively.

Designing catalysts that can effectively make use of renewable energy benefits to solve the current challenges of environmental pollution and increasing energy demands. Piezo-photocatalysis that can utilize solar energy and natural vibration-energy has emerged as a \"green\" technique. In this work, we fabricated BiFeO3/C nano composites that can harvest solar and vibration energies and degrade organic pollutants. The incorporated carbon quantum dots bring about more efficient visible light absorbance and separation of photoinduced electron-hole pairs. The piezoelectric polarization further suppresses the recombination of photoinduced electron-hole pairs. The catalysts own higher reaction rates in piezo-photocatalysis and the BiFeO3/C-0.12 shows the highest degradation efficiency (k-value of 0.0835 min-1).

OBJECTIVE: To evaluate the effect of adding vanillin nanoparticles on the antimicrobial and mechanical properties of the orthodontic adhesive.
METHODS: Transbond XT orthodontic adhesive (3M Unitek, Monrovia, California, USA) was modified with 1% and 2% vanillin nanoparticles. The chemical composition and degree of chemical conversion in orthodontic adhesive before and after adding vanillin nanoparticles to orthodontic adhesive were measured using Fourier transformation infrared spectroscopy (FTIR). Mechanical properties of unmodified orthodontic adhesive (UMOA) and 1% and 2% vanillin-modified orthodontic adhesive (VMOA) were assessed in shear bond strength (SBS) and tensile bond strength (TBS). The antimicrobial properties were evaluated using a Mueller-Hinton plate swapped with streptococcus mutans. The zone of bacterial inhibition for UMOA, 1% VMOA, and 2% VMOA was measured. Descriptive statistics, multiple comparisons, one-way ANOVA, and post hoc Duncan\'s test were used to compare among the results.
RESULTS: FTIR showed no chemical conversion of 1% VMOA and 2% VMOA. There was significant streptococcus mutans growth inhibition in 1% VMOA and 2% VMOA compared to UMOA. No significant difference in streptococcus mutans growth inhibition in 1% VMOA and 2% VMOA. The SBS decreased significantly in 1% VMOA compared to UMOA. In addition, SBS decreased insignificantly when comparing 1% VMOA and 2% VMOA. TBS significantly reduced in 2% VMOA compared with UMOA. In addition, there was no significant difference in TBS between UMOA and 1% VMOA, and 1% VMOA and 2% VMOA, respectively.
CONCLUSIONS: The 1% VMOA has improved antimicrobial properties and kept mechanical properties of orthodontic adhesive within the acceptable level.

Hybrid nanofibers of a chitosan-polycaprolactone blend containing titanium dioxide nanoparticles TiO2NPs, were prepared through electrospinning to study their adsorption and photocatalytic degradation capabilities of the model organic water pollutants, rhodamine B, RhB. To obtain uniform and bead-free nanofibers, an optimization of the electrospinning parameters was performed. The optimization was carried out by systematically adjusting the solution conditions (solvent, concentration, and polymer ratio) and instrumental parameters (voltage, needle tip-collector distance, and flow). The obtained materials were characterized by FT-IR, TGA, DSC, SEM, TEM, mechanical tensile test, and water contact angle. The photoactivity was investigated using a batch-type system by following UV-Vis absorbance and fluorescence of RhB. TiO2NPs were incorporated ex-situ into the polymer matrix, contributing to good mechanical properties and higher hydrophilicity of the material. The results showed that the presence of chitosan in the nanofibers significantly increased the adsorption of RhB and its photocatalytic degradation by TiO2NPs (5, 55 and 80 % of RhB degradation with NFs of PCL, TiO2/PCL and TiO2/CS-PCL, after 30 h of light irradiation, respectively), evidencing a synergistic effect between them. The results are attributed to an attraction of RhB by chitosan to the vicinity of TiO2NPs, favouring initial adsorption and degradation, phenomenon known as \"bait-and-hook-and-destruct\" effect.

Microplastics and pesticides are emerging contaminants in the marine biota, which cause many harmful effects on aquatic organisms, especially on fish. Fish is a staple and affordable food source, rich in animal protein, along with various vitamins, essential amino acids, and minerals. Exposure of fish to microplastics, pesticides, and various nanoparticles generates ROS and induces oxidative stress, inflammation, immunotoxicity, genotoxicity, and DNA damage and alters gut microbiota, thus reducing the growth and quality of fish. Changes in fish behavioral patterns, swimming, and feeding habits were also observed under exposures to the above contaminants. These contaminants also affect the Nrf-2, JNK, ERK, NF-κB, and MAPK signaling pathways. And Nrf2-KEAP1 signalling modulates redox status marinating enzymes in fish. Effects of pesticides, microplastics, and nanoparticles found to modulate many antioxidant enzymes, including superoxide dismutase, catalase, and glutathione system. So, to protect fish health from stress, the contribution of nano-technology or nano-formulations was researched. A decrease in fish nutritional quality and population significantly impacts on the human diet, influencing traditions and economics worldwide. On the other hand, traces of microplastics and pesticides in the habitat water can enter humans by consuming contaminated fish which may result in serious health hazards. This review summarizes the oxidative stress caused due to microplastics, pesticides and nano-particle contamination or exposure in fish habitat water and their impact on human health. As a rescue mechanism, the use of nano-technology in the management of fish health and disease was discussed.

This study is aimed to optimise the preparation factors, such as sonication time (5-20 min), cholesterol to lecetin ratio (CHLR) (0.2-0.8), and essential oil content (0.1-0.3 g/100 g) in solvent evaporation method for formulation of liposomal nanocarriers containing garlic essential oil (GEO) in order to find the highest encapsulation efficiency and stability with strongest antioxidant and antimicrobial activity. The droplet size, zeta potential, encapsulation efficiency, turbidity, changes in turbidity after storage (as a measure of instability), antioxidant capacity, and antimicrobial activity were measured for all prepared samples of nanoliposome. The sonication time is recognised as the most effective factor on the droplet size, zeta potential, encapsulation efficiency, turbidity, and instability while CHLR was the most effective factor on zeta potential and instability. The content of GEO significantly affected the antioxidant and antimicrobial activity in particular against gram-negative bacteria (Escherichia coli). The results of FTIR based on the identification of functional groups confirmed the presence of GEO in the spectra of the prepared nanoliposome and also it was not observed the interaction between the components of the nanoliposome. The overall optimum conditions were determined by response surface methodology (RSM) as the predicted values of the studied factors (sonication time: 18.99 min, CHLR: 0.59 and content of GEO: 0.3 g/100 g) based on obtaining the highest stability and efficiency as well as strongest antioxidant and antimicrobial activity.

The rapid development of nanomedicine has increased the likelihood that manufactured nanoparticles will one day come into contact with people and the environment. A variety of academic fields, including engineering and the health sciences, have taken a keen interest in the development of nanotechnology. Any significant development in nanomaterial-based applications would depend on the production of functionalized nanoparticles, which are believed to have the potential to be used in fields like pharmaceutical and biomedical sciences. The functionalization of nanoparticles with particular recognition chemical moieties does result in multifunctional nanoparticles with greater efficacy while at the same time minimising adverse effects, according to early clinical studies. This is because of traits like aggressive cellular uptake and focused localization in tumours. To advance this field of inquiry, chemical procedures must be developed that reliably attach chemical moieties to nanoparticles. The structure-function relationship of these functionalized nanoparticles has been extensively studied as a result of the discovery of several chemical processes for the synthesis of functionalized nanoparticles specifically for drug delivery, cancer therapy, diagnostics, tissue engineering, and molecular biology. Because of the growing understanding of how to functionalize nanoparticles and the continued work of innovative scientists to expand this technology, it is anticipated that functionalized nanoparticles will play an important role in the aforementioned domains. As a result, the goal of this study is to familiarise readers with nanoparticles, to explain functionalization techniques that have already been developed, and to examine potential applications for nanoparticles in the biomedical sciences. This review\'s information is essential for the safe and broad use of functionalized nanoparticles, particularly in the biomedical sector.