未经鉴定:从中国陕西省长庆油田的废水中分离出兼性厌氧菌厦门希瓦氏菌CQ-Y1。希瓦氏杆菌是重要的异化金属还原菌。它在生物降解和生物修复中显示出良好的潜在应用。
未经批准:基因组测序,进行组装和功能注释以探索CQ-Y1的基因组信息。通过紫外可见分光光度法研究了温度和NaCl浓度对CQ-Y1生长和Fe(III)还原的影响,SEM和XRD。
UNASSIGNED:基因组分析显示其完整的基因组是4,710,887bp的环状染色体,GC含量为46.50%和4,110个CDS基因,86个tRNAs和26个rRNAs。它包含编码Na+/H+反转运蛋白的基因,K+/Cl-转运蛋白,热休克蛋白与NaCl和耐高温性有关。与黄素相关的基因的存在,细胞色素c,铁载体,和其他相关蛋白支持Fe(III)还原。此外,CQ-Y1可以在10%NaCl(w/v)和45°C下存活,与NaCl浓度相比,温度对细菌生长的影响更为明显。在不含NaCl的情况下,CQ-Y1在30°C下的最大Fe(III)还原率达到70.1%,并且还原反应在40°C和3%NaCl(w/v)下保持活性。NaCl浓度比温度对微生物Fe(III)还原更有效。在高温和高NaCl条件下的还原产物为Fe3(PO4)2,FeCl2和Fe(OH)2。
未经批准:因此,假设细胞色素c和黄素介导的CQ-Y1的Fe(III)还原机制。这些发现可以为更好地了解厦门S的基因组和代谢多样性的起源和进化提供信息。
UNASSIGNED: A facultative anaerobe bacterium Shewanella xiamenensis CQ-Y1 was isolated from the wastewater of Changqing oilfield in Shaanxi Province of China. Shewanella is the important dissimilatory metal-reducing bacteria. It exhibited a well potential application in biodegradation and bioremediation.
UNASSIGNED: Genome sequencing, assembling and functional annotation were conducted to explore the genome information of CQ-Y1. The effect of temperatures and NaCl concentrations on the CQ-Y1 growth and Fe(III) reduction were investigated by UV visible spectrophotometry, SEM and XRD.
UNASSIGNED: Genomic analysis revealed its complete genome was a circular chromosome of 4,710,887 bp with a GC content of 46.50% and 4,110 CDSs genes, 86 tRNAs and 26 rRNAs. It contains genes encoding for Na+/H+ antiporter, K+/Cl- transporter, heat shock protein associated with NaCl and high-temperature resistance. The presence of genes related to flavin, Cytochrome c, siderophore, and other related proteins supported Fe(III) reduction. In addition, CQ-Y1 could survive at 10% NaCl (w/v) and 45°C, and temperature showed more pronounced effects than NaCl concentration on the bacterial growth. The maximum Fe(III) reduction ratio of CQ-Y1 reached 70.1% at 30°C without NaCl, and the reduction reaction remained active at 40°C with 3% NaCl (w/v). NaCl concentration was more effective than temperature on microbial Fe(III) reduction. And the reduction products under high temperature and high NaCl conditions were characterized as Fe3(PO4)2, FeCl2 and Fe(OH)2.
UNASSIGNED: Accordingly, a Fe(III) reduction mechanism of CQ-Y1 mediated by Cytochrome c and flavin was hypothesised. These findings could provide information for a better understanding of the origin and evolution of genomic and metabolic diversity of S. xiamenensis.