PDF(Pubmed)
Abstract：
Previously, we isolated a novel lactic acid bacteria species (Lactobacillus nasalidis) from the fresh forestomach contents of a captive proboscis monkey (Nasalis larvatus) in a Japanese zoo. In this study, we isolated two strains of L. nasalidis from the freeze-dried forestomach contents of a wild proboscis monkey inhabiting a riverine forest in Malaysia. The samples had been stored for more than six years. Phenotypic analysis showed that strains isolated from the wild individual had more diverse sugar utilization and lower salt tolerance than strains previously isolated from the captive counterpart. These phenotypic differences are most likely induced by feeding conditions; wild individuals consume a wide variety of natural food, unlike their zoo-raised counterparts that consume formula feed with sodium sufficiency. Since 16s rRNA sequences of L. nasalidis were detected in the previously created 16S rRNA libraries of wild, provisioned, and captive proboscis monkeys in Malaysia and Japan, L. nasalidis may be an essential bacterium of the foregut microbial community of the proboscis monkey. The currently established method for the isolation of gut bacteria from freeze-dried samples under storage will be applicable to many already-stored precious samples.
摘要：
以前,我们从日本动物园的圈养长鼻猴（Nasalislarvatus）的新鲜前胃内容物中分离出一种新型乳酸菌（Nasalislarvatus）。在这项研究中,我们从居住在马来西亚河流森林中的野生长鼻猴的冻干前胃内容物中分离出了两株纳氏乳杆菌。这些样品已经储存了六年多。表型分析表明，与先前从圈养对应物中分离的菌株相比，从野生个体中分离的菌株具有更多的糖利用率和更低的耐盐性。这些表型差异很可能是由摄食条件引起的；野生个体食用各种各样的天然食物，与动物园饲养的同行不同，后者食用含钠充足的配方饲料。由于在先前创建的野生型16SrRNA文库中检测到了纳氏乳杆菌的16SrRNA序列，预配置，马来西亚和日本圈养的长鼻猴，纳氏乳杆菌可能是长鼻猴前肠微生物群落的必需细菌。目前建立的从储存的冻干样品中分离肠道细菌的方法将适用于许多已经储存的珍贵样品。
