背景:SARS-CoV-2是COVID-19的病原体,是一种与地方性人类冠状病毒(hCoV)OC43和HKU1同属的β冠状病毒,与αhCoV229E和NL63不同。在一项针对菲律宾青少年的研究中,我们评估了hCoV的血清阳性率,大流行前的hCoV免疫是否调节了SARS-CoV-2感染的后续风险,以及SARS-CoV-2感染是否影响了hCoV的传播。
方法:从2021年收集的499个样本中,通过SARS-CoV-2受体结合域(RBD)酶联免疫吸附测定(ELISA)进行筛选,我们随机选择59例SARS-CoV-2阴性和61例阳性个体进行进一步的血清学评估。我们通过ELISA在大流行前(2018-2019年)和大流行中期(2021年)收集的相同参与者的样本中测量了对四种hCoV和SARS-CoV-2的RBD和尖峰抗体。在COVID-19疫苗接种前。
结果:我们观察到4种hCoV大流行前血清阳性超过72%。结合抗体随年龄增加至229E和OC43,提示地方病循环,而HKU1和NL63的豁免权在不同年龄段持平。在COVID-19大流行期间,在SARS-CoV-2阴性和阳性青少年中,OC43,NL63和229E的RBD以及所有四种hCoV的峰值抗体水平显着增加。2021年12-15岁的人群对RBD的抗体和OC43,NL63和229E的峰值高于2019年同龄青少年,进一步证明了在大流行期间hCoV的强烈传播。
结论:我们观察到COVID-19大流行对地方性hCoV传播的影响有限。这项研究为hCoV和SARS-CoV-2的共同循环提供了见解。
UNASSIGNED: SARS-CoV-2, the causative agent of COVID-19, is a betacoronavirus belonging to the same genus as endemic human coronaviruses (hCoVs) OC43 and HKU1 and is distinct from alpha hCoVs 229E and
NL63. In a study of adolescents in the Philippines, we evaluated the seroprevalence to hCoVs, whether pre-pandemic hCoV immunity modulated subsequent risk of SARS-CoV-2 infection, and if SARS-CoV-2 infection affected the transmission of the hCoVs.
UNASSIGNED: From 499 samples collected in 2021 and screened by SARS-CoV-2 receptor binding domain (RBD) enzyme-linked immunosorbent assay (ELISA), we randomly selected 59 SARS-CoV-2 negative and 61 positive individuals for further serological evaluation. We measured RBD and spike antibodies to the four hCoVs and SARS-CoV-2 by ELISA in samples from the same participants collected pre-pandemic (2018-2019) and mid-pandemic (2021), before COVID-19 vaccination.
UNASSIGNED: We observed over 72% seropositivity to the four hCoVs pre-pandemic. Binding antibodies increased with age to 229E and OC43, suggesting endemic circulation, while immunity was flat across ages for HKU1 and
NL63. During the COVID-19 pandemic, antibody level increased significantly to the RBDs of OC43,
NL63, and 229E and spikes of all four hCoVs in both SARS-CoV-2 negative and positive adolescents. Those aged 12-15 years old in 2021 had higher antibodies to RBD and spike of OC43,
NL63, and 229E than adolescents the same age in 2019, further demonstrating intense transmission of the hCoVs during the pandemic.
UNASSIGNED: We observe a limited impact of the COVID-19 pandemic on endemic hCoV transmission. This study provides insight into co-circulation of hCoVs and SARS-CoV-2.