未经证实:在视网膜色素上皮(RPE)的基底层(BL)和Bruch膜的内层胶原层(亚RPE-BL空间)之间的细胞外沉积物中发现了由羟基磷灰石或白铁矿形成的微米大小的球体。这项调查旨在表征形态学特征,结构,以及这些球体在有或没有年龄相关性黄斑变性(AMD)临床指征的老年人眼中的分布。
未经评估:实验研究。
UNASSIGNED:从伦敦大学学院眼科研究所和Moorfield的眼科医院组织库或AdvancingSightNetwork获得了五只具有不同程度的亚RPE-BL沉积物的人眼。据报道有两只眼睛有AMD的临床指征(年龄,76-87岁),而3人被认为是健康的(年龄,69-91岁)。
UNASSIGNED:将具有亚RPE-BL沉积物的尸体眼包埋在石蜡中,并切成4-10μm的厚度。使用高分辨率扫描电子显微镜(SEM)鉴定和表征球,能量色散X射线光谱,和飞行时间二次离子质谱。
UNASSIGNED:球体的高分辨率扫描电子显微照片,球粒的大小-频率分布,包括平均直径,以及颗粒在中心-外周轴上的分布。还获得了元素图和飞行时间二次离子质谱。
未经评估:球体的沉淀遍布中部,中间外围,和老年人眼的远轴。沿该轴的小球频率没有发现显着差异。然而,统计分析表明,在这些区域中,小球表现出明显不同的大小。深入分析显示,具有AMD临床征象的眼睛的亚RPE-BL间隙中的小球明显更大(中值直径,1.64μm)比健康老年人眼(中位直径,1.16μm)。最后,球粒的表面形貌和内部结构变化较大。
UNASSIGNED:在人的眼睛中,亚RPE-BL空间中的球体沉淀在中心-外围轴上普遍存在。然而,与没有AMD临床症状的眼睛相比,在有AMD临床症状的眼睛中,球体的大小和频率存在显着差异,提示球体的大小和频率可能与AMD有关。
UNASSIGNED: Micrometer-sized spherules formed of hydroxyapatite or whitlockite were identified within extracellular deposits that accumulate in the space between the basal lamina (BL) of retinal pigment epithelium (RPE) and the inner collagenous layer of Bruch\'s membrane (sub-RPE-BL space). This investigation aimed to characterize the morphologic features, structure, and distribution of these spherules in aged human eyes with and without clinical indications of age-related macular degeneration (AMD).
UNASSIGNED: Experimental study.
UNASSIGNED: Five human eyes with varying degrees of sub-RPE-BL deposits were obtained from the University College London Institute of Ophthalmology and Moorfield\'s Eye Hospital Tissue Repository or the Advancing Sight Network. Two eyes were reported as having clinical indications of AMD (age, 76-87 years), whereas 3 were considered healthy (age, 69-91 years).
UNASSIGNED: Cadaveric eyes with sub-RPE-BL deposits were embedded in paraffin wax and sectioned to a thickness of 4-10 μm. Spherules were identified and characterized using high-resolution scanning electron microscopy (SEM), energy-dispersive x-ray spectroscopy, and time-of-flight secondary ion mass spectroscopy.
UNASSIGNED: High-resolution scanning electron micrographs of spherules, the size-frequency distribution of spherules including average diameter, and the distribution of particles across the central-peripheral axis. Elemental maps and time-of-flight secondary ion mass spectra also were obtained.
UNASSIGNED: The precipitation of spherules is ubiquitous across the central, mid-peripheral, and far-peripheral axis in aged human eyes. No significant difference was found in the frequency of spherules along this axis. However, statistical analysis indicated that spherules exhibited significantly different sizes in these regions. In-depth analysis revealed that spherules in the sub-RPE-BL space of eyes with clinical signs of AMD were significantly larger (median diameter, 1.64 μm) than those in healthy aged eyes (median diameter, 1.16 μm). Finally, spherules showed great variation in surface topography and internal structure.
UNASSIGNED: The precipitation of spherules in the sub-RPE-BL space is ubiquitous across the central-peripheral axis in aged human eyes. However, a marked difference exists in the size and frequency of spherules in eyes with clinical signs of AMD compared to those without, suggesting that the size and frequency of spherules may be associated with AMD.