本研究旨在探讨鸽子成肌分化1(MYOD1)基因多态性与car体性状及其表达与胸肌发育的相关性。在鸽子MYOD1基因中发现了四个SNP。相关分析表明,AA基因型个体在两个SNPsg.2967A>G(p<.01)和g.3044G>A(p<.05)具有显著较高的活重(LW)。car体重量(CW),半内脏重量(SEW),内脏重量(EW)和胸肌重量(宝马)。此外,两种SNP对鸽子胸肌MYOD1mRNA表达水平也有同样显著的影响,ie,AA基因型显示更高的MYOD1mRNA表达水平。肌纤维的直径和横截面面积从0w连续增加到4w(p<0.05),伴随着MYOD1基因表达的增加,而密度从0w大幅下降(p<.05)到1w,并在接下来的几周内持续下降(p>.05)。更重要的是,MYOD1基因的表达水平与肌纤维的直径(r=0.937,p<0.05)和横截面积(r=0.956,p<0.01)呈正相关,与密度呈负相关(r=-0.769,p<0.01)。结果表明,在SNPsg.2967A>G和g.3044G>A处具有AA基因型的个体均表现出较高的car体性状(LW,CW,SEW,电子战,和BMW),胸肌中的MYOD1mRNA表达水平高于AB和BB基因型。此外,MYOD1基因表达水平与肌肉特征性状密切相关,提示MYOD1基因的变异与肌肉发育密切相关,可能是标记辅助选择鸽子的潜在候选基因。
This study was to investigate the correlations of myogenic differentiation 1 (
MYOD1) gene polymorphisms with carcass traits and its expression with breast muscle development in pigeons. Four SNPs were found in the pigeon
MYOD1 gene. Correlation analysis showed that individuals with AA genotype at both SNPs g.2967A > G (p < .01) and g.3044G > A (p < .05) have significantly higher live weight (LW), carcass weight (CW), semi-eviscerated weight (SEW), eviscerated weight (EW) and breast muscle weight (BMW). Moreover, the two SNPs also had the same significant effects on
MYOD1 mRNA expression levels in breast muscle of pigeons, ie, the AA genotype showed higher
MYOD1 mRNA expression levels. The diameter and cross-section area of muscle fibers continuously increased from 0w to 4w (p < .05), accompanied with the increasing expression of MYOD1 gene, while the density decreased (p < .05) dramatically from 0w to 1w and continuously fell over in the next few weeks (p > .05). What\'s more, the expression level of
MYOD1 gene was positively correlated with a diameter (r = 0.937, p < .05) and cross-sectional area (r = 0.956, p < .01) of myofiber, and negatively correlated with density (r = -0.769, p < .01). The results showed that individuals with AA genotype at both SNPs g.2967A > G and g.3044G > A have showed higher carcass traits (LW, CW, SEW, EW, and BMW) and higher MYOD1 mRNA expression level in breast muscle than AB and BB genotypes. Moreover, the expression level of MYOD1 gene was closely correlated with muscle characteristic traits, indicating variants of MYOD1 gene was closely related to muscle development and could be a potential candidate gene in marker-assisted selection of pigeons.