目的:抗生素耐药性的上升引发了人们对分枝杆菌噬菌体作为分枝杆菌感染的替代治疗策略的新兴趣。到目前为止,绝大多数的分枝杆菌噬菌体都是使用模型物种耻垢分枝杆菌分离的,这意味着环境中绝大多数分枝杆菌噬菌体仍未培养,未分类,它们的具体宿主和感染策略仍然未知。进行这项研究以分离和表征针对败血分枝杆菌的新型分枝杆菌噬菌体。
方法:从武汉的土壤样品中分离出一种新型的抗败血分枝杆菌噬菌体WXIN,中国。全基因组分析表明,噬菌体基因组由115,158bp组成,GC含量为61.9%。在260个推定的开放阅读框架中,46可能与噬菌体包装有关,结构,裂解,溶源性,基因组修饰/复制,和其他功能角色。有限的全基因组相似性,以及基于病毒蛋白质组和直系同源基因构建的系统发育树表明,噬菌体WXIN代表与簇J分枝杆菌噬菌体(Omegavirus属)远近相关的新型簇。总的来说,这些结果为分枝杆菌噬菌体的基因组特性提供了新的见解,突出分枝杆菌噬菌体与其宿主相关的巨大遗传多样性。
OBJECTIVE: The rising of antibiotic resistance has sparked a renewed interest in mycobacteriophage as alternative therapeutic strategies against mycobacterial infections. So far, the vast majority of
mycobacteriophages have been isolated using the model species Mycobacterium smegmatis, implying an overwhelming majority of
mycobacteriophages in the environment remain uncultured, unclassified, and their specific hosts and infection strategies are still unknown. This study was undertaken to isolate and characterize novel
mycobacteriophages targeting Mycobacterium septicum.
METHODS: Here a novel mycobacteriophage WXIN against M. septicum was isolated from soil samples in Wuhan, China. Whole genome analysis indicates that the phage genome consists of 115,158 bp with a GC content of 61.9%. Of the 260 putative open reading frames, 46 may be associated with phage packaging, structure, lysis, lysogeny, genome modification/replication, and other functional roles. The limited genome-wide similarity, along with phylogenetic trees constructed based on viral proteome and orthologous genes show that phage WXIN represents a novel cluster distantly related to cluster J mycobacteriophages (genus Omegavirus). Overall, these results provide novel insights into the genomic properties of
mycobacteriophages, highlighting the great genetic diversity of
mycobacteriophages in relation to their hosts.