Abstract：
Bacteriophages infecting Mycobacterium smegmatis mc2155 are numerous and, hence, are classified into clusters based on nucleotide sequence similarity. Analyzing phages belonging to clusters/subclusters can help gain deeper insights into their biological features and potential therapeutic applications. In this study, for genomic characterization of B1 subcluster mycobacteriophages, a framework of online tools was developed, which enabled functional annotation of about 55% of the previously deemed hypothetical proteins in B1 phages. We also studied the phenotype, lysogeny status, and antimycobacterial activity of 10 B1 phages against biofilm and an antibiotic-resistant M. smegmatis strain (4XR1). All 10 phages belonged to the Siphoviridae family, appeared temperate based on their spontaneous release from the putative lysogens and showed antibiofilm activity. The highest inhibitory and disruptive effects on biofilm were 64% and 46%, respectively. This systematic characterization using a combination of genomic and experimental tools is a promising approach to furthering our understanding of viral dark matter.
摘要：
感染耻垢分枝杆菌mc2155的噬菌体数量众多，因此，根据核苷酸序列相似性分为簇。分析属于簇/子簇的噬菌体可以帮助获得对其生物学特征和潜在治疗应用的更深入了解。在这项研究中,对于B1亚簇分枝杆菌噬菌体的基因组表征，开发了一个在线工具框架，这使得在B1噬菌体中约55％的先前认为的假设蛋白质的功能注释成为可能。我们还研究了表型，溶源状态，和10个B1噬菌体抗生物膜和抗生素抗性耻垢分枝杆菌菌株（4XR1）的抗分枝杆菌活性。所有10个噬菌体都属于虹彩科，基于它们从推定的溶原中自发释放而显得温和，并显示出抗生物膜活性。对生物膜的抑制作用和破坏作用最高，分别为64％和46％，分别。使用基因组和实验工具的组合的这种系统表征是促进我们对病毒暗物质的理解的有希望的方法。
