金属耐受性蛋白(MTP)家族的成员在介导二价金属阳离子的转运和耐受性方面至关重要。尽管意义重大,对芥菜(芥菜)中MTP基因的理解仍然有限,特别是关于它们对重金属(HM)胁迫的反应。在我们的研究中,我们鉴定了芸苔属中的MTP基因集(17个基因),黑油菜(18个基因),和使用HMMER(阳离子流出;PF01545)和BLAST分析的结球芽孢杆菌(33个基因)。对于33个BjMTP,全面的生物信息学分析,涵盖物理化学性质,系统发育关系,保守的图案,蛋白质结构,共线性,时空RNA-seq表达,GO富集,在六个HM应力(Mn2+,Fe2+,Zn2+,Cd2+,Sb3+,和Pb2+)进行。根据物理化学特征的发现,系统发育树,和共线性,异源多倍体结球芽孢杆菌的MTP基因遗传自其祖细胞,B.rapa和B.nigra,在多倍体化过程中基因损失最小。BjMTP家族的成员表现出保守的基序,启动子元件,和跨子组的表达模式,与MTP的七个进化分支(G1、G4-G9和G12)一致。Further,HM胁迫下的时空表达谱分析成功地鉴定了与BjMTP对HM胁迫的响应相关的特定基因和关键顺式调节元件。这些发现可能有助于改良双歧杆菌的遗传,以增强HM耐受性,促进修复受HM污染的地区。
Members of the Metal Tolerance Protein (MTP) family are critical in mediating the transport and tolerance of divalent metal cations. Despite their significance, the understanding of MTP genes in mustard (Brassica juncea) remains limited, especially regarding their response to heavy metal (HM) stress. In our study, we identified MTP gene sets in Brassica rapa (17 genes), Brassica nigra (18 genes), and B. juncea (33 genes) using the HMMER (Cation_efflux; PF01545) and BLAST analysis. For the 33 BjMTPs, a comprehensive bioinformatics analysis covering the physicochemical properties, phylogenetic relationships, conserved motifs, protein structures, collinearity, spatiotemporal RNA-seq expression, GO enrichment, and expression profiling under six HM stresses (Mn2+, Fe2+, Zn2+, Cd2+, Sb3+, and Pb2+) were carried out. According to the findings of physicochemical characteristics, phylogenetic tree, and collinearity, the allopolyploid B. juncea\'s MTP genes were inherited from its progenitors, B. rapa and B. nigra, with minimal gene loss during polyploidization. Members of the BjMTP family exhibited conserved motifs, promoter elements, and expression patterns across subgroups, consistent with the seven evolutionary branches (G1, G4-G9, and G12) of the MTPs. Further, spatiotemporal expression profiling under HM stresses successfully identified specific genes and crucial cis-regulatory elements associated with the response of BjMTPs to HM stresses. These findings may contribute to the genetic improvement of B. juncea for enhanced HM tolerance, facilitating the remediation of HM-contaminated areas.