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Abstract：
Leaf mustard (Brassica juncea L. Czern & Coss), an important vegetable crop, experiences pronounced adversity due to seasonal drought stress, particularly at the seed germination stage. Although there is partial comprehension of drought-responsive genes, the role of long non-coding RNAs (lncRNAs) in adjusting mustard\'s drought stress response is largely unexplored. In this study, we showed that the drought-tolerant cultivar \'Weiliang\' manifested a markedly lower base water potential (-1.073 MPa vs -0.437 MPa) and higher germination percentage (41.2% vs 0%) than the drought-susceptible cultivar \'Shuidong\' under drought conditions. High throughput RNA sequencing techniques revealed a significant repertoire of lncRNAs from both cultivars during germination under drought stress, resulting in the identification of 2,087 differentially expressed lncRNAs (DELs) and their correspondingly linked 12,433 target genes. It was noted that 84 genes targeted by DEL exhibited enrichment in the photosynthesis pathway. Gene network construction showed that MSTRG.150397, a regulatory lncRNA, was inferred to potentially modulate key photosynthetic genes (Psb27, PetC, PetH, and PsbW), whilst MSTRG.107159 was indicated as an inhibitory regulator of six drought-responsive PIP genes. Further, weighted gene co-expression network analysis (WGCNA) corroborated the involvement of light intensity and stress response genes targeted by the identified DELs. The precision and regulatory impact of lncRNA were verified through qPCR. This study extends our knowledge of the regulatory mechanisms governing drought stress responses in mustard, which will help strategies to augment drought tolerance in this crop.
摘要：
芥菜（BrassicajunceaL.Czern和Coss），一种重要的蔬菜作物，由于季节性干旱压力，经历了明显的逆境，特别是在种子发芽阶段。虽然对干旱响应基因有部分理解，长链非编码RNA（lncRNAs）在调节芥菜的干旱胁迫反应中的作用在很大程度上尚未被研究。在这项研究中,我们表明，与干旱易感品种“水东”相比，耐旱品种“威良”表现出明显较低的基础水势（-1.073MPavs-0.437MPa）和较高的发芽率（41.2％vs0％）。干旱条件下。高通量RNA测序技术揭示了干旱胁迫下萌发过程中来自两个品种的lncRNAs的大量库。结果鉴定了2,087个差异表达的lncRNAs（DELs）及其相应连接的12,433个靶基因。注意到DEL靶向的84个基因在光合作用途径中表现出富集。基因网络的构建表明,MSTRG.150397,一种调控lncRNA,被推断可能调节关键的光合基因(Psb27，PetC，PetH,和PsbW），而MSTRG.107159被指示为六个干旱响应性PIP基因的抑制调节因子。Further,加权基因共表达网络分析（WGCNA）证实了所确定的DEL靶向的光强度和应激反应基因的参与。通过qPCR验证了lncRNA的精确性和调控影响。这项研究扩展了我们对芥菜干旱胁迫反应调控机制的认识，这将有助于提高这种作物耐旱性的策略。
