■当地的保健医生已经使用本港榕树来治疗疼痛,炎症,风湿病,和其他健康问题。
■在这项研究中,粗提物和不同的馏分,与分离的F.benghalensis化合物一起检查了它们作为肌肉松弛剂的作用,镇痛药,和镇静剂。
■对提取物和分离的化合物1进行肌肉松弛剂筛选,镇痛药,和镇静行动。乙酸介导的扭体模型用于镇痛评估,肌肉松弛潜力通过牵引和倾斜计划测试进行量化,并应用野外试验观察镇静效果。
以各种剂量测试提取物/级分(25、50和100mg/kg)和分离的化合物(2.5、5、10和20mg/kg)。在乙酸介导的扭体模型中,观察到对carpachromene(64.44%)的明显减少(p<0.001),随后是乙酸乙酯(60.67%)和甲醇(58.42%)级分。对分离的化合物(71.09%)观察到明显的(p<0.001)肌肉松弛活性,随后是乙酸乙酯(66.98%)和甲醇(67.10%)级分。关于镇静作用,对分离的化合物(71.09%)有显著作用,随后是乙酸乙酯(66.98%)和甲醇(67.10%)级分。此外,使用分子对接探索分离化合物的结合模式。分子对接研究表明,分离的化合物对COX2和GABA具有良好的结合亲和力。我们分离的化合物可能对COX2和GABA受体具有抑制活性。
■无花果的提取物和分离的化合物可用作镇痛药,肌肉松弛剂,和镇静剂。然而,详细的分子和功能分析对于确定它们作为肌肉松弛剂的功能至关重要,镇痛药,和镇静剂。
UNASSIGNED: Ficus benghalensis has been used by local health care practitioners to treat pain, inflammation, rheumatism, and other health issues.
UNASSIGNED: In this study, the crude extract and diverse fractions, along with the isolated compound of F. benghalensis were examined for their roles as muscle relaxants, analgesics, and sedatives.
UNASSIGNED: The extract and isolated compound 1 were screened for muscle-relaxant, analgesic, and sedative actions. The acetic acid-mediated writhing model was utilized for analgesic assessment, the muscle relaxant potential was quantified through traction and inclined plan tests, and the open field test was applied for sedative effects.
UNASSIGNED: The extract/fractions (25, 50, and 100 mg/kg) and isolated compounds (2.5, 5, 10, and 20 mg/kg) were tested at various doses. A profound (p< 0.001) reduce in the acetic acid-mediated writhing model was observed against carpachromene (64.44%), followed by ethyl acetate (60.67%) and methanol (58.42%) fractions. A marked (p< 0.001) muscle relaxant activity was noticed against the isolated compound (71.09%), followed by ethyl acetate (66.98%) and methanol (67.10%) fractions. Regarding the sedative effect, a significant action was noted against the isolated compound (71.09%), followed by ethyl acetate (66.98%) and methanol (67.10%) fractions. Furthermore, the binding modes of the isolated compounds were explored using molecular docking. The molecular docking study revealed that the isolated compound possessed good binding affinity for COX2 and GABA. Our isolated compound may possess inhibitory activity against COX2 and GABA receptors.
UNASSIGNED: The extract and isolated compounds of Ficus benghalensis can be used as analgesics, muscle relaxants, and sedatives. However, detailed molecular and functional analyses are essential to ascertain their function as muscle relaxants, analgesics, and sedatives.