PDF(Pubmed)
Abstract：
UNASSIGNED: Pistacia chinensis is extensively employed in traditional medicine. This study aimed to isolate and evaluate the therapeutic effects of 3\'4\'78-tetrahydroxy-3-methoxyflavone from P. chinensis crude extract.
UNASSIGNED: The study utilized column chromatography for isolation. The plant extract and its isolated compound were assessed for in vivo analgesic (hot plate model), anti-inflammatory (carrageenan-induced paw edema), sedative (open field model), and muscle relaxing properties (inclined plane and traction test).
UNASSIGNED: In the thermal-induced analgesic model, a significant analgesic effect was observed for the extract (25, 50, and 100 mg/kg) and the isolated compound (2.5, 5, 10, and 15 mg/kg) at higher doses. The extract (100 mg/kg) significantly prolonged latency time (21.98 seconds) after 120 minutes of administration. The isolated compound elevated the latency time (20.03 seconds) after 30 minutes, remaining significant up to 120 minutes with a latency time of 24.11 seconds. The anti-inflammatory effect showed a reduction in inflammatory reactions by 50.23% (extract) and 67.09% (compound) after the fifth hour of treatment. Both samples demonstrated significant sedative effects, with the extract hindering movement by 54.11 lines crossed compared to the negative control (180.99 lines). The isolated compound reduced the number of lines crossed to 15.23±SEM compared to the negative control. Both samples were also significant muscle relaxants. Docking studies indicated that the compound\'s therapeutic effect is due to inhibiting COX and nociceptive pathways.
UNASSIGNED: The isolated compound from Pistacia chinensis exhibits significant analgesic, anti-inflammatory, sedative, and muscle relaxing properties, with potential therapeutic applications by inhibiting COX and nociceptive pathways.
UNASSIGNED: The study utilized column chromatography for isolation. The plant extract and its isolated compound were assessed for in vivo analgesic (hot plate model), anti-inflammatory (carrageenan-induced paw edema), sedative (open field model), and muscle relaxing properties (inclined plane and traction test).
UNASSIGNED: In the thermal-induced analgesic model, a significant analgesic effect was observed for the extract (25, 50, and 100 mg/kg) and the isolated compound (2.5, 5, 10, and 15 mg/kg) at higher doses. The extract (100 mg/kg) significantly prolonged latency time (21.98 seconds) after 120 minutes of administration. The isolated compound elevated the latency time (20.03 seconds) after 30 minutes, remaining significant up to 120 minutes with a latency time of 24.11 seconds. The anti-inflammatory effect showed a reduction in inflammatory reactions by 50.23% (extract) and 67.09% (compound) after the fifth hour of treatment. Both samples demonstrated significant sedative effects, with the extract hindering movement by 54.11 lines crossed compared to the negative control (180.99 lines). The isolated compound reduced the number of lines crossed to 15.23±SEM compared to the negative control. Both samples were also significant muscle relaxants. Docking studies indicated that the compound\'s therapeutic effect is due to inhibiting COX and nociceptive pathways.
UNASSIGNED: The isolated compound from Pistacia chinensis exhibits significant analgesic, anti-inflammatory, sedative, and muscle relaxing properties, with potential therapeutic applications by inhibiting COX and nociceptive pathways.
摘要：
■黄连木在传统医学中被广泛使用。本研究旨在分离和评价黄连粗提物中3'4'78-四羟基-3-甲氧基黄酮的治疗效果。
■该研究利用柱色谱法进行分离。评估植物提取物及其分离的化合物的体内镇痛（热板模型），抗炎（角叉菜胶诱导的爪水肿），镇静剂(露天模型)，和肌肉放松性能（倾斜平面和牵引试验）。
■在热诱导镇痛模型中，提取物(25,50和100mg/kg)和分离的化合物(2.5,5,10和15mg/kg)在较高剂量下观察到显著的镇痛效果.给药120分钟后，提取物（100mg/kg）显着延长了潜伏期（21.98秒）。分离的化合物在30分钟后延长了潜伏期（20.03秒），保持显著长达120分钟，延迟时间为24.11秒。抗炎作用显示在治疗的第5小时后,炎性反应减少50.23%(提取物)和67.09%(化合物)。两个样本都显示出显著的镇静作用,与阴性对照（180.99线）相比，提取物阻碍了54.11线的移动。与阴性对照相比,分离的化合物将交叉线的数目减少至15.23±SEM。两个样品也是显著的肌肉松弛剂。对接研究表明，该化合物的治疗效果是由于抑制COX和伤害性途径。
■从黄连木中分离的化合物具有显着的镇痛作用，抗炎,镇静剂,和肌肉放松的特性，通过抑制COX和伤害性途径具有潜在的治疗应用。
■该研究利用柱色谱法进行分离。评估植物提取物及其分离的化合物的体内镇痛（热板模型），抗炎（角叉菜胶诱导的爪水肿），镇静剂(露天模型)，和肌肉放松性能（倾斜平面和牵引试验）。
■在热诱导镇痛模型中，提取物(25,50和100mg/kg)和分离的化合物(2.5,5,10和15mg/kg)在较高剂量下观察到显著的镇痛效果.给药120分钟后，提取物（100mg/kg）显着延长了潜伏期（21.98秒）。分离的化合物在30分钟后延长了潜伏期（20.03秒），保持显著长达120分钟，延迟时间为24.11秒。抗炎作用显示在治疗的第5小时后,炎性反应减少50.23%(提取物)和67.09%(化合物)。两个样本都显示出显著的镇静作用,与阴性对照（180.99线）相比，提取物阻碍了54.11线的移动。与阴性对照相比,分离的化合物将交叉线的数目减少至15.23±SEM。两个样品也是显著的肌肉松弛剂。对接研究表明，该化合物的治疗效果是由于抑制COX和伤害性途径。
■从黄连木中分离的化合物具有显着的镇痛作用，抗炎,镇静剂,和肌肉放松的特性，通过抑制COX和伤害性途径具有潜在的治疗应用。
