%0 Journal Article
%T Neurite outgrowth promotion in PC12 cells by 24(R)-ethyllophenol from Morus alba.
%A Honma S
%A Handa K
%A Mitsumata R
%A Shimada D
%A Takei S
%A Tanaka G
%A Watanabe K
%A Yoshida M
%J Pharmazie
%V 79
%N 3
%D 2024 May 15
%M 38872272
%F 1.515
%R 10.1691/ph.2024.3656
%X We examined the mechanism by which 24(R)-ethyllophenol (MAB28) isolated from the branches of Morus alba caused neurite outgrowth in rat pheochromocytoma cells (PC12). MAB28 significantly promoted neurite outgrowth to a similar degree as the positive control, nerve growth factor (NGF). After incubation with MAB28 in PC12 cells, phosphorylation of extracellular signal-regulated kinase, p38 mitogen-activated protein kinase, and cyclic AMP response element-binding protein was detected, but the time course of phosphorylation was different from that induced by NGF. The expression of chloride intracellular channel protein 3 (CLIC3) was significantly decreased by MAB28. 5-Nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB), an outward rectifying chloride channel inhibitor, significantly promoted neurite outgrowth in PC12 cells. These data suggested that MAB28 could induce neurite outgrowth by downregulating CLIC3 expression.