背景:辣木的叶子。,阿育吠陀几千年的药用和食用草药,在身体清洁过程中和之后用作Pancha(na)业力(泻药),治疗便秘,降低胆固醇和体重。
目的:探讨油茶叶对便秘大鼠腹泻的影响及其可能机制。
方法:制备油菌叶的热水提取物(WEMOL),并使用LC-20ATHPLC系统进行分析。用低纤维饲料喂养21天,建立大鼠便秘模型。口服WEMOL7天后,排泄参数分析,胃肠推进,通过HE和阿尔辛蓝染色进行组织学分析,用ELISA法检测大鼠消化道胃肠激素,评价WEMOL的通便作用。用LC-MS/MS进行无标记定量(LFQ),生物信息学和Westernblot用于发现和验证WEMOL与腹泻相关的信号通路和关键蛋白。
结果:异槲皮苷和黄芪苷的含量分别为2.7mg/g和1.7mg/g,分别在WEMOL。凳子号,体重,便秘大鼠的含水量明显降低,指示模型已成功建立。WEMOL(1.25和2.5g/kg)增加了粪便的含水量和气体水平,MTL,NPY在胃窦和VIP,便秘大鼠结肠中的SP,改善结肠肌层厚度和粘蛋白分泌。蛋白质组学共揭示了1731种差异蛋白和9条信号通路,WEMOL增加了Vamp2、Glai3和Prkacb的表达。
结论:WEMOL的通便机制可能调控5-HT和Ach受体介导的信号通路,与胃肠蠕动和肠液分泌有关。它可以被认为是阿育吠陀油茶叶的Pancha(na)业力的科学内涵。
BACKGROUND: The leaf of Moringa oleifera Lam., a medicinal and edible herb for thousands of years in Ayurveda, is used as Pancha (na) karma (purgative) during and after the body cleansing process, to treat constipation, reduce cholesterol and body weight.
OBJECTIVE: The aim was to investigate the diarrhea effects and possible mechanism of M. oleifera leaves in constipation rats.
METHODS: The hot water extract of M. oleifera leaves (WEMOL) was prepared and analyzed using LC-20AT HPLC system. The constipated rat model was induced by feeding with low fiber diet for 21 days. After oral administration of WEMOL for 7 days, the excretion parameter analysis, gastro-intestinal propulsion, histological analysis by HE and Alcian blue staining, and gastrointestinal hormone in rat\'s digestive tract through ELISA were used to evaluate the laxative effect of WEMOL. Label-free quantitation (LFQ) with LC-MS/MS, bioinformatics and Western blot were used to discover and verify the signal pathways and key proteins of WEMOL related to diarrhea.
RESULTS: The contents of isoquercitrin and astragalin were 2.7 mg/g and 1.7 mg/g, respectively in WEMOL. The stool number, weight, and water content of constipation rats were significantly reduced, indicating model had been established successfully. WEMOL (1.25 and 2.5 g/kg) increased water content of feces and the levels of Gas, MTL, NPY in gastric antrum and VIP, SP in colon of constipation rats, improved the muscle layer thickness and mucin secretion of colon. The proteomics revealed a total of 1731 differential proteins and 9 signaling pathways, WEMOL increased the expression of Vamp2, Gnai3, and Prkacb.
CONCLUSIONS: The laxative mechanism of WEMOL maybe modulate the signaling pathways mediated by 5-HT and Ach receptors, related to gastrointestinal motility and intestinal fluid secretion. It can be considered as the scientific connotation of Pancha (na) karma of M. oleifera leaves in ayurveda.