由于其优异的抗氧化和抗肥胖特性,岩藻黄质是食品和制药工业中的通用物质。几种微藻,包括巴夫洛娃物种。,可以生产岩藻黄质,是潜在的工业岩藻黄质生产者,因为它们缺乏坚硬的细胞壁,这有利于岩藻黄质的提取。然而,Pavlovaspp的商业应用。由于生物质生产不足而受到限制。在这项研究中,我们旨在开发一种混合营养培养方法,以增加PavlovagyransOPMS30543X的生物量和岩藻黄质产量。不同有机碳源培养OPMS30543X的效果,甘油浓度,混合营养条件,和光强度对有机碳源消耗的影响,生物质生产,并分析了岩藻黄质的积累。几种有机碳源,如甘油,葡萄糖,蔗糖,和醋酸盐,被检查,揭示甘油被微藻充分消耗。与没有甘油时观察到的相比,OPMS30543X的生物质和岩藻黄质生产在10mM甘油存在下增加。代谢组学分析显示与糖酵解相关的代谢物水平较高,卡尔文-本森-巴斯舍姆,和三羧酸在兼养条件下比在自养条件下循环。在兼养条件下以100µmol光子m-2s-1的光强度生长的培养物比自养培养物产生更多的岩藻黄质。值得注意的是,到目前为止,Pavlova物种的岩藻黄质产量(18.9mg/L)最高。总之,使用混合营养培养是增加巴夫洛娃物种岩藻黄质产量的有希望的策略。关键点:•甘油增强了巴甫洛瓦州的生物量和岩藻黄质生产•在混合营养条件下代谢物水平增加•混合营养条件和中等光照强度适用于P.gyrans。
Fucoxanthin is a versatile substance in the food and pharmaceutical industries owing to its excellent antioxidant and anti-obesity properties. Several microalgae, including the haptophyte Pavlova spp., can produce fucoxanthin and are potential industrial fucoxanthin producers, as they lack rigid cell walls, which facilitates fucoxanthin extraction. However, the commercial application of Pavlova spp. is limited owing to insufficient biomass production. In this study, we aimed to develop a
mixotrophic cultivation method to increase biomass and fucoxanthin production in Pavlova gyrans OPMS 30543X. The effects of culturing OPMS 30543X with different organic carbon sources, glycerol concentrations, mixed-nutrient conditions, and light intensities on the consumption of organic carbon sources, biomass production, and fucoxanthin accumulation were analyzed. Several organic carbon sources, such as glycerol, glucose, sucrose, and acetate, were examined, revealing that glycerol was well-consumed by the microalgae. Biomass and fucoxanthin production by OPMS 30543X increased in the presence of 10 mM glycerol compared to that observed without glycerol. Metabolomic analysis revealed higher levels of the metabolites related to the glycolytic, Calvin-Benson-Bassham, and tricarboxylic acid cycles under
mixotrophic conditions than under autotrophic conditions. Cultures grown under
mixotrophic conditions with a light intensity of 100 µmol photons m-2 s-1 produced more fucoxanthin than autotrophic cultures. Notably, the amount of fucoxanthin produced (18.9 mg/L) was the highest reported thus far for Pavlova species. In conclusion, the use of
mixotrophic culture is a promising strategy for increasing fucoxanthin production in Pavlova species. KEY POINTS: • Glycerol enhances biomass and fucoxanthin production in Pavlova gyrans • Metabolite levels increase under mixotrophic conditions •
Mixotrophic conditions and medium-light intensity are appropriate for P. gyrans.