线粒体在卵泡发育和排卵中起着至关重要的作用,至少部分通过生长激素(GH)/胰岛素样生长因子-1(IGF-1)对线粒体生物发生的作用。这项研究旨在确定麝香大鼠(Ondatrazibethicus)卵巢中GH/IGF-1系统和线粒体生物发生的季节性变化。我们利用了麝鼠,典型的季节性饲养员,使用免疫组织化学阐明GH/IGF-1系统对不同繁殖季节线粒体生物发生的潜在影响,基因表达和高通量测序。在繁殖季节(BS)和非繁殖季节(NBS)之间,麝香大鼠卵巢中存在卵泡发育的变化,伴随着循环和卵巢GH和IGF-1浓度的显着降低。GH,GHR,IGF-1,IGF-1R,线粒体生物发生标记在两个季节都定位在麝香大鼠的卵巢细胞中。相比之下,Gh,Ghr,Igf-1,Igf-1r,ppargc1a,ppargc1b,Tfam,BS中Nrf1/2mRNA水平较高。循环和卵巢中GH和IGF-1的相对水平与线粒体生物发生标志物呈正相关。此外,RNA-seq分析表明,差异表达基因可能与胰岛素和PI3K/Akt信号通路有关。以及线粒体功能相关途径。这些发现表明,卵巢内GH/IGF-1系统,这与线粒体生物发生的季节性变化有关,在BS的麝香大鼠卵巢中被激活。
Mitochondria play a critical role in follicular development and ovulation, at least in part through the actions of growth hormone (GH)/insulin-like growth factor-1 (IGF-1) on mitochondrial biogenesis. This study aimed to identify seasonal alterations in the GH/IGF-1 system and mitochondrial biogenesis in muskrat (Ondatra zibethicus) ovaries. We utilized the muskrat, a typical seasonal breeder, to clarify the potential impact of the GH/IGF-1 system on mitochondrial biogenesis across different breeding seasons using immunohistochemistry, gene expression and high-throughput sequencing. Alterations in follicular development existed in muskrat ovaries between the breeding season (BS) and non-breeding season (NBS), accompanied by a striking decrease in circulating and ovarian GH and IGF-1 concentrations. GH, GHR, IGF-1, IGF-1R, and mitochondrial biogenesis markers were localized in the ovarian cells of muskrats during both seasons. In contrast, Gh, Ghr, Igf-1, Igf-1r, Ppargc1a, Ppargc1b, Tfam, and Nrf1/2 mRNA levels were higher in BS. The relative levels of GH and IGF-1 in circulation and ovaries were positively associated with mitochondrial biogenesis markers. Additionally, RNA-seq analysis demonstrated that differentially expressed genes might be associated with insulin and PI3K/Akt signaling pathways, as well as mitochondrial function-related pathways. These findings suggest that the intra-ovarian GH/IGF-1 system, which is associated with seasonal changes in mitochondrial biogenesis, is activated in muskrat ovaries in BS.