益生菌在大嘴鲈鱼(Micropterussalmoides)水产养殖部门中起着至关重要的作用。它们帮助鱼类预防疾病,肠道结构改善,食物吸收,和免疫系统的加强。在这个实验中,枯草芽孢杆菌(BS,107CFU/g)和罗伊乳杆菌(LR,将107CFU/g)添加到饲料中,然后将其喂入M.salmoides35天。两种益生菌对生长的影响,豁免权,并对S菌的代谢进行了研究。结果表明,BS组显着提高了M.salmoides的生长速率和比生长速率,而BS和LR组均显着增加绒毛M.salmoides肠的长度。BS组AKP水平显著升高,T-AOC,和猫在沙尔米德氏杆菌的血液中,以及肠道中的AKP水平。此外,BS组肠道基因Nrf2、SOD1、GPX的表达显著增加,CAT,同时显著降低keap1基因的表达。沙氏M.salmoides肠道微生物分析表明,对照组和实验组的Planctomycetota丰度显着不同。在属一级分析,丰富的柠檬酸杆菌,Paracocus,Luedemannella,Sphingomonas,对照组和实验组的链霉菌和黄单胞菌均存在显着差异。BS组的差异表达基因主要富集在肠道的氧化磷酸化途径中,表明它们对肠道代谢和炎症抑制有良好的影响。相比之下,LR组中的差异表达基因主要富集在胰岛素信号传导和亚油酸代谢途径中,表明改善肠道代谢性能。总之,B.subtilis和罗伊氏乳杆菌改善了M.salmoides的生长和健康,显示了增强肠道代谢的巨大潜力,具有显著的应用价值。
Probiotics play an essential role in the largemouth bass (Micropterus salmoides) aquaculture sector. They aid the fish in sickness prevention, intestinal structure improvement, food absorption, and immune system strengthening. In this experiment, Bacillus subtilis (BS, 107 CFU/g) and Lactobacillus reuteri (LR, 107 CFU/g) were added to the feed and then fed to M. salmoides for 35 days. The effects of two probiotics on the growth, immunity, and metabolism of M. salmoides organisms were studied. The results revealed that the BS group significantly increased the growth rate and specific growth rate of M. salmoides, while both the BS and LR groups significantly increase the length of villi M. salmoides intestines. The BS group significantly increased the levels of AKP, T-AOC, and CAT in the blood of M. salmoides, as well as AKP levels in the intestine. Furthermore, the BS group significantly increased the expression of intestinal genes Nrf2, SOD1, GPX, and CAT, while significantly decreasing the expression of the keap1 gene. M. salmoides gut microbial analysis showed that the abundance of Planctomycetota was significantly different in both control and experimental groups. Analyzed at the genus level, the abundance of Citrobacter, Paracoccus, Luedemannella, Sphingomonas, Streptomyces and Xanthomonas in the both control and experimental groups were significantly different. The BS group\'s differentially expressed genes were predominantly enriched in oxidative phosphorylation pathways in the intestine, indicating that they had a good influence on intestinal metabolism and inflammation suppression. In contrast, differentially expressed genes in the LR group were primarily enriched in the insulin signaling and linoleic acid metabolism pathways, indicating improved intestine metabolic performance. In conclusion, B. subtilis and L. reuteri improve the growth and health of M. salmoides, indicating tremendous potential for enhancing intestinal metabolism and providing significant application value.