Abstract：
This study provided evidence that the inclusion of hydrolysable tannin (HT) in high soybean meal (SBM) diets improved growth performance and glycolipid metabolism of largemouth bass (Micropterus salmoides). In vivo, various levels of HT were added to high SBM diets and fed to largemouth bass (initial weight: 6.00 ± 0.03 g) for 56 days. Results showed that a high level of SBM led to the reduction in growth performance, as evidenced by decreased weight gain rate and impaired hepatic function. Dietary supplementation with HT (1.0 g/kg) improved growth performance of largemouth bass, accompanied by the enhancements in hepatic antioxidant capacity and glycolipid metabolism. In vitro, HT facilitated glucose utilization in hepatocytes and positively influenced the modulation of crucial genes within the PI3K/Akt signaling pathway. Conversely, administration of LY294002 (a PI3K inhibitor) reversed the detrimental effects observed in hepatocytes exposed to high glucose levels. Overall, incorporating HT (1.0 g/kg) into the diet enhanced liver health and improved the absorption and utilization of SBM in largemouth bass, potentially achieved through modulation of the PI3K/Akt signaling pathway.
摘要：
这项研究提供了证据，表明在高豆粕（SBM）日粮中加入可水解单宁（HT）可以改善大嘴鲈鱼（Micropterussalmoides）的生长性能和糖脂代谢。在体内,将各种水平的HT添加到高SBM日粮中，并饲喂大口鲈鱼（初始重量：6.00±0.03g）56天。结果表明，高水平的SBM导致生长性能下降，体重增加率下降和肝功能受损证明了这一点。日粮补充HT（1.0g/kg）可改善大口鲈鱼的生长性能，伴随着肝脏抗氧化能力和糖脂代谢的增强。体外，HT促进肝细胞中的葡萄糖利用，并积极影响PI3K/Akt信号通路内关键基因的调节。相反，施用LY294002(PI3K抑制剂)逆转了在暴露于高葡萄糖水平的肝细胞中观察到的有害作用。总的来说,将HT（1.0g/kg）掺入饮食中可增强肝脏健康，并改善大口鲈鱼对SBM的吸收和利用，可能通过调节PI3K/Akt信号通路来实现。
