微生物区系在微生物强化技术中起着重要作用。这些试剂的污染物降解能力和可行数量对于保证其实际应用至关重要。在这项研究中,筛选出高效降解污染物的微生物菌群,对其培养基成分和培养条件进行了优化,并在沸石滴滤塔中验证了其效果。在最佳条件下培养24小时后,活菌计数达到4.76×109cfu/mL,氨氮(NH4+-N)的降解率,硝酸盐氮(NO3--N),总氮(TN),总磷(TP),化学需氧量(COD)增加到93.5%,100%,68.3%,32.6%,85%,分别。优化喂养策略后,活菌浓度达到5.80×109cfu/mL。在应用效果验证实验中,NH4+-N的降解率,TN,TP,实验组COD达到96.69%,75.18%,73.82%,90.83%,分别,与对照组的结果相比,显示出显着的改善。对照组主要成分为Dokdonella,Brevundimonas,Alishewanella,红杆菌属,假黄单胞菌,和Thauera,而实验组的人是Dokdonella,变形杆菌,红杆菌属,Dechlomonas,和硝基螺旋体。变形杆菌,Dechlomonas,和Nitrosra,这是实验组独有的,是用于脱氮除磷的常见细菌。这解释了两组之间污水处理能力的差异。本研究提供了一种替代的污水处理微生物菌群,具有合理的生产成本和较高的降解效率,TN,TP,和COD。
Microbial flora plays an important role in microorganism-enhanced technology. The pollutant degradation ability and viable counts of these agents are crucial to guarantee their practical application. In this study, an efficient pollutant-degrading microbial flora was screened, its medium components and culture conditions were optimized, and its effect was verified in zeolite trickling filter towers. After a 24 h culture under the optimal conditions, the viable count reached 4.76 × 109 cfu/mL, with the degradation rates of ammonia nitrogen (NH4+-N), nitrate nitrogen (NO3--N), total nitrogen (TN), total phosphorus (TP), and chemical oxygen demand (COD) increased to 93.5%, 100%, 68.3%, 32.6%, and 85%, respectively. After optimizing the feeding strategy, the concentration of viable bacteria reached 5.80 × 109 cfu/mL. In the application effect verification experiment, the degradation rates of NH4+-N, TN, TP, and COD in the experimental group reached 96.69%, 75.18%, 73.82%, and 90.83%, respectively, showing a significant improvement compared to the results of the control group. The main components in the control group were Dokdonella, Brevundimonas, Alishewanella, Rhodobacter, Pseudoxanthomonas, and Thauera, whereas those in the experimental group were Dokdonella, Proteocatella, Rhodobacter, Dechlomonas, and Nitrospira. Proteocatella, Dechlomonas, and Nitrosra, which were unique to the experimental group, are common bacteria used for nitrogen and phosphorus removal. This explains the difference in the sewage treatment capacity between the two groups. This study provides an alternative sewage treatment microbial flora with a reasonable production cost and high degradation efficiency for NH4+-N, TN, TP, and COD.