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Abstract：
UNASSIGNED: Endodontic microbial flora plays a pivotal role in the development and persistence of periodontal endodontic lesions (PELs). Understanding the composition and prevalence of microbial species in PELs is essential for effective treatment strategies.
UNASSIGNED: Microbial samples were collected from 50 teeth diagnosed with PELs. Sterile paper points were used to obtain samples from the root canals. Deoxyribonucleic acid (DNA) was extracted and subjected to polymerase chain reaction (PCR) amplification of the 16S ribosomal RNA (rRNA) gene to identify bacterial species. The obtained data were analyzed using statistical methods.
UNASSIGNED: The microbial analysis revealed a diverse range of bacterial species in PELs. The most prevalent species were Porphyromonas gingivalis (32.5%), Treponema denticola (28.0%), and Fusobacterium nucleatum (22.5%). Streptococcus mutans (9.0%) and Actinomyces naeslundii (8.0%) were also frequently detected. Additionally, Prevotella intermedia (7.0%), Aggregatibacter actinomycetemcomitans (3.5%), and Enterococcus faecalis (2.5%) were present in lower frequencies.
UNASSIGNED: The presence of a diverse microbial flora in teeth with PELs underscores the polymicrobial nature of these lesions. The predominance of periodontal pathogens such as Porphyromonas gingivalis, Treponema denticola, and Fusobacterium nucleatum suggests a strong association between periodontal and endodontic infections. A comprehensive understanding of the microbial profile in PELs is crucial for tailored therapeutic approaches targeting the specific pathogens involved.
UNASSIGNED: Microbial samples were collected from 50 teeth diagnosed with PELs. Sterile paper points were used to obtain samples from the root canals. Deoxyribonucleic acid (DNA) was extracted and subjected to polymerase chain reaction (PCR) amplification of the 16S ribosomal RNA (rRNA) gene to identify bacterial species. The obtained data were analyzed using statistical methods.
UNASSIGNED: The microbial analysis revealed a diverse range of bacterial species in PELs. The most prevalent species were Porphyromonas gingivalis (32.5%), Treponema denticola (28.0%), and Fusobacterium nucleatum (22.5%). Streptococcus mutans (9.0%) and Actinomyces naeslundii (8.0%) were also frequently detected. Additionally, Prevotella intermedia (7.0%), Aggregatibacter actinomycetemcomitans (3.5%), and Enterococcus faecalis (2.5%) were present in lower frequencies.
UNASSIGNED: The presence of a diverse microbial flora in teeth with PELs underscores the polymicrobial nature of these lesions. The predominance of periodontal pathogens such as Porphyromonas gingivalis, Treponema denticola, and Fusobacterium nucleatum suggests a strong association between periodontal and endodontic infections. A comprehensive understanding of the microbial profile in PELs is crucial for tailored therapeutic approaches targeting the specific pathogens involved.
摘要：
■牙髓微生物菌群在牙周牙髓病变（PEL）的发展和持续中起着关键作用。了解PEL中微生物物种的组成和流行对于有效的治疗策略至关重要。
■从诊断为PEL的50颗牙齿收集微生物样本。使用无菌纸点从根管获得样品。提取脱氧核糖核酸（DNA），并对16S核糖体RNA（rRNA）基因进行聚合酶链反应（PCR）扩增，以鉴定细菌种类。使用统计学方法对获得的数据进行分析。
■微生物分析揭示了PEL中多种细菌种类。最普遍的物种是牙龈卟啉单胞菌（32.5％），Denticola密螺旋体（28.0%），和具核梭杆菌(22.5%)。还经常检测到变形链球菌（9.0％）和放线菌（8.0％）。此外,中间普氏菌(7.0%)，放线菌聚集杆菌（3.5%），粪肠球菌（2.5％）的频率较低。
■带有PEL的牙齿中存在多种微生物菌群，这突显了这些病变的多微生物性质。牙周病原体的优势，如牙龈卟啉单胞菌，Denticola密螺旋体,和核梭杆菌提示牙周和牙髓感染之间有很强的关联。全面了解PEL中的微生物谱对于针对所涉及的特定病原体的定制治疗方法至关重要。
■从诊断为PEL的50颗牙齿收集微生物样本。使用无菌纸点从根管获得样品。提取脱氧核糖核酸（DNA），并对16S核糖体RNA（rRNA）基因进行聚合酶链反应（PCR）扩增，以鉴定细菌种类。使用统计学方法对获得的数据进行分析。
■微生物分析揭示了PEL中多种细菌种类。最普遍的物种是牙龈卟啉单胞菌（32.5％），Denticola密螺旋体（28.0%），和具核梭杆菌(22.5%)。还经常检测到变形链球菌（9.0％）和放线菌（8.0％）。此外,中间普氏菌(7.0%)，放线菌聚集杆菌（3.5%），粪肠球菌（2.5％）的频率较低。
■带有PEL的牙齿中存在多种微生物菌群，这突显了这些病变的多微生物性质。牙周病原体的优势，如牙龈卟啉单胞菌，Denticola密螺旋体,和核梭杆菌提示牙周和牙髓感染之间有很强的关联。全面了解PEL中的微生物谱对于针对所涉及的特定病原体的定制治疗方法至关重要。
