Globally, fungal infections have evolved as a strenuous challenge for clinicians, particularly in patients with compromised immunity in intensive care units. Fungal co-infection in Covid-19 patients has made the situation more formidable for healthcare practitioners. Surface adhered fungal population known as biofilm often develop at the diseased site to elicit antifungal tolerance and recalcitrant traits. Thus, an innovative strategy is required to impede/eradicate developed biofilm and avoid the formation of new colonies. The development of nanocomposite-based antibiofilm solutions is the most appropriate way to withstand and dismantle biofilm structures. Nanocomposites can be utilized as a drug delivery medium and for fabrication of anti-biofilm surfaces capable to resist fungal colonization. In this context, the present review comprehensively described different forms of nanocomposites and mode of their action against fungal biofilms. Amongst various nanocomposites, efficacy of metal/organic nanoparticles and nanofibers are particularly emphasized to highlight their role in the pursuit of antibiofilm strategies. Further, the inevitable concern of nanotoxicology has also been introduced and discussed with the exigent need of addressing it while developing nano-based therapies. Further, a list of FDA-approved nano-based antifungal formulations for therapeutic usage available to date has been described. Collectively, the review highlights the potential, scope, and future of nanocomposite-based antibiofilm therapeutics to address the fungal biofilm management issue.

The pathogenesis of chronic wounds (CW) involves a multifaceted interplay of biochemical, immunological, hematological, and microbiological interactions. Biofilm development is a significant virulence trait which enhances microbial survival and pathogenicity and has various implications on the development and management of CW. Biofilms induce a prolonged suboptimal inflammation in the wound microenvironment, associated with delayed healing. The composition of wound fluid (WF) adds more complexity to the subject, with proven pro-inflammatory properties and an intricate crosstalk among cytokines, chemokines, microRNAs, proteases, growth factors, and ECM components. One approach to achieve information on the mechanisms of disease progression and therapeutic response is the use of multiple high-throughput \'OMIC\' modalities (genomic, proteomic, lipidomic, metabolomic assays), facilitating the discovery of potential biomarkers for wound healing, which may represent a breakthrough in this field and a major help in addressing delayed wound healing. In this review article, we aim to summarize the current progress achieved in host-microbiome crosstalk in the spectrum of CW healing and highlight future innovative strategies to boost the host immune response against infections, focusing on the interaction between pathogens and their hosts (for instance, by harnessing microorganisms like probiotics), which may serve as the prospective advancement of vaccines and treatments against infections.

Maintenance of the quality and hygiene of maxillofacial prosthesis allows to maintain the health of the residual tissues. Sampling of the maxillofacial prostheses has relieved presence of microbial colonization on silicone surfaces. Cleaning procedures of maxillofacial silicones are done using mechanical means or using adjunctive with chemical means. Cleaning with a 2-4% chlorhexidine gluconate spray or dipping in solution for a minute and then washing under running water can sufficiently condition to reduce the amount of bacterial contamination. Due to rising microorganism resistance and fewer adverse effects, phytoextracts appear to be a viable option. Additionally, the use of excipients derived from plants is provides new opportunities for the pharmaceutical industry into the creation of innovative pharmaceutical products that are sustainable.
UNASSIGNED: To evaluate and compare the leaf extracts of Mangifera indica (M.indica), Anacardium occidentale(A.occidentale) and 0.2% chlorhexidine gluconate (CHX) on disinfection of maxillofacial silicone material surface contaminated with Staphylococcus aureus (S.aureus) and Candida albicans (C.albicans).
UNASSIGNED: Of the 150 maxillofacial silicone elastomer silicone samples, 75 samples were contaminated with S. aureus and 75 with C.albicans. The contaminated disc was rolled on blood agar and pre-disinfection Colony Forming Units (CFU) were evaluated followed by subjecting the discs to disinfection protocols. The contaminated discs with S. aureus and C.albicans were disinfected using M.indica leaf extracts, A.occidentale leaf extracts and 0.2% CHX for 10 min. Post-disinfection CFUs were evaluated by rolling the disc on blood agar. The results were tabulated and analysed using dependent t-test, one-way ANOVA and Tukeys multiple posthoc procedure.
UNASSIGNED: Pair-wise comparison of pre-and post-disinfection log CFU counts of S.aureus gave a statistical significance between 0.2% CHX and and M.indica leaf extract. No statistically significant results were found between 0.2% CHX and A.occidentale. Pair wise comparison of the log CFU from pre-disinfection to post-disinfection of C.albicans gave a statistical significance between all the three groups.
UNASSIGNED: In the present study A.occidentale leaf extract and M.indica leaf extract have shown significant reduction in CFU of both the organisms. 0.2% CHX showed the most CFU reduction post disinfection of maxillofacial silicone material surface contaminated S.aureus and C.albicans followed by A.occidentale leaf extracts and M.indica leaf extracts. Given the limitations of the current research, A.occidentale leaf extract and M.indica leaf extract can be used as an alternative for disinfection of maxillofacial silicone prosthesis.

The blue mussel Mytilus edulis is a widespread and abundant bivalve species along the North Sea with high economic and ecological importance as an engineer species. The shell of mussels is intensively colonized by microbial organisms that can produce significant quantities of nitrous oxide (N2O), a potent greenhouse gas. To characterize the impacts of climate change on the composition, structure and functioning of microbial biofilms on the shell surface of M. edulis, we experimentally exposed them to orthogonal combinations of increased seawater temperature (20 vs. 23 °C) and decreased pH (8.0 vs. 7.7) for six weeks. We used amplicon sequencing of the 16S rRNA gene to characterize the alpha and beta diversity of microbial communities on the mussel shell. The functioning of microbial biofilms was assessed by measuring aerobic respiration and nitrogen emission rates. We did not report any significant impacts of climate change treatments on the diversity of mussel microbiomes nor on the structure of these communities. Lowered pH and increased temperature had antagonistic effects on the functioning of microbial communities with decreased aerobic respiration and N2O emission rates of microbial biofilms in acidified seawater compared to increased rates in warmer conditions. An overriding impact of acidification over warming was finally observed on N2O emissions when the two factors were combined. Although acidification and warming in combination significantly reduced N2O biofilm emissions, the promotion of aquaculture activities in coastal waters where shellfish do not normally occur at high biomass and density could nonetheless result in unwanted emissions of this greenhouse gas in a near future.

Microbial biofilms are prevalent in various environments and pose significant challenges to food safety and public health. The biofilms formed by pathogens can cause food spoilage, foodborne illness, and infectious diseases, which are difficult to treat due to their enhanced antimicrobial resistance. While the composition and development of biofilms have been widely studied, their profound impact on food, the food industry, and public health has not been sufficiently recapitulated. This review aims to provide a comprehensive overview of microbial biofilms in the food industry and their implication on public health. It highlights the existence of biofilms along the food-producing chains and the underlying mechanisms of biofilm-associated diseases. Furthermore, this review thoroughly summarizes the enhanced understanding of microbial biofilms achieved through machine learning approaches in biofilm research. By consolidating existing knowledge, this review intends to facilitate developing effective strategies to combat biofilm-associated infections in both the food industry and public health.

The utilization of nanoparticles derived from algae has generated increasing attention owing to their environmentally sustainable characteristics and their capacity to interact harmoniously with biologically active metabolites. The present study utilized P. boergesenii for the purpose of synthesizing copper oxide nanoparticles (CuONPs), which were subsequently subjected to in vitro assessment against various bacterial pathogens and cancer cells A375. The biosynthesized CuONPs were subjected to various analytical techniques including FTIR, XRD, HRSEM, TEM, and Zeta sizer analyses in order to characterize their stability and assess their size distribution. The utilization of Fourier Transform Infrared (FTIR) analysis has provided confirmation that the algal metabolites serve to stabilize the CuONPs and function as capping agents. The X-ray diffraction (XRD) analysis revealed a distinct peak associated with the (103) plane, characterized by its sharpness and high intensity, indicating its crystalline properties. The size of the CuONPs in the tetragonal crystalline structure was measured to be 76 nm, and they exhibited a negative zeta potential. The biological assay demonstrated that the CuONPs exhibited significant antibacterial activity when tested against both Bacillus subtilis and Escherichia coli. The cytotoxic effects of CuONPs and cisplatin, when tested at a concentration of 100 µg/mL on the A375 malignant melanoma cell line, were approximately 70% and 95%, respectively. The CuONPs that were synthesized demonstrated significant potential in terms of their antibacterial properties and their ability to inhibit the growth of malignant melanoma cells.

This work reports biochemical characterization of Thermothelomyces thermophilus cellobiose dehydrogenase (TthCDHIIa) and its application as an antimicrobial and antibiofilm agent. We demonstrate that TthCDHIIa is thermostable in different ionic solutions and is capable of oxidizing multiple mono and oligosaccharide substrates and to continuously produce H2O2. Kinetics measurements depict the enzyme catalytic characteristics consistent with an Ascomycota class II CDH. Our structural analyses show that TthCDHIIa substrate binding pocket is spacious enough to accommodate larger cello and xylooligosaccharides. We also reveal that TthCDHIIa supplemented with cellobiose reduces the viability of S. aureus ATCC 25923 up to 32 % in a planktonic growth model and also inhibits its biofilm growth on 62.5 %. Furthermore, TthCDHIIa eradicates preformed S. aureus biofilms via H2O2 oxidative degradation of the biofilm matrix, making these bacteria considerably more susceptible to gentamicin and tetracycline.

Biofilm-associated infections are a critical element in infectious diseases and play an important role in antibiotic resistance. Biosynthesized gold nanoparticles (AuNPs) using ethanolic extract of Musa sapientum unripe fruit were performed. The nanoparticles demonstrated an absorption peak at 554 nm with particle sizes ranging from 5.45 to 104.44 nm. High negative zeta potential value of -33.97 mV confirmed the high stability of AuNPs. The presence of bioconstituents responsible for capping and stabilization was indicated by intensity changes of several peaks from Fourier-transform infrared spectroscopy analysis. The minimum inhibitory concentrations (MIC) of the biosynthesized AuNPs against important pathogens ranged from 10 to 40 μg mL-1 . Synthesized nanoparticles at 0.062 to 0.5 × MIC significantly inhibited biofilm formation in all the tested microorganisms (p < 0.05). Scanning electron microscopy and confocal scanning laser microscopy images clearly illustrated in disruption and architectural changes of microbial biofilms at sub-MIC of biosynthesized AuNPs. Excellent antioxidant and antityrosinase activities of AuNPs were observed. The biosynthesized AuNPs at 20 μg mL-1 significantly inhibited nitric oxide production by 93% in lipopolysaccharide-stimulated RAW 264.7 cells, compared with control (p < 0.05). The biosynthesized AuNPs at 0.6 to 40 μg mL-1 demonstrated no toxic effects on L929 fibroblast cells.

In microbial biofilms, bacterial cells are encased in a self-produced matrix of polymers (e.g., exopolysaccharides) that enable surface adherence and protect against environmental stressors. For example, the wrinkly spreader phenotype of Pseudomonas fluorescens colonizes food/water sources and human tissue to form robust biofilms that can spread across surfaces. This biofilm largely consists of bacterial cellulose produced by the cellulose synthase proteins encoded by the wss (WS structural) operon, which also occurs in other species, including pathogenic Achromobacter species. Although phenotypic mutant analysis of the wssFGHI genes has previously shown that they are responsible for acetylation of bacterial cellulose, their specific roles remain unknown and distinct from the recently identified cellulose phosphoethanolamine modification found in other species. Here, we have purified the C-terminal soluble form of WssI from P. fluorescens and Achromobacter insuavis and demonstrated acetylesterase activity with chromogenic substrates. The kinetic parameters (kcat/KM values of 13 and 8.0 M-1 s-1, respectively) indicate that these enzymes are up to four times more catalytically efficient than the closest characterized homolog, AlgJ from the alginate synthase. Unlike AlgJ and its cognate alginate polymer, WssI also demonstrated acetyltransferase activity onto cellulose oligomers (e.g., cellotetraose to cellohexaose) with multiple acetyl donor substrates (p-nitrophenyl acetate, 4-methylumbelliferyl acetate, and acetyl-CoA). Finally, a high-throughput screen identified three low micromolar WssI inhibitors that may be useful for chemically interrogating cellulose acetylation and biofilm formation.

Since the birth of civilization, people have recognized that infectious microbes cause serious and often fatal diseases in humans. One of the most dangerous characteristics of microorganisms is their propensity to form biofilms. It is linked to the development of long-lasting infections and more severe illness. An obstacle to eliminating such intricate structures is their resistance to the drugs now utilized in clinical practice (biofilms). Finding new compounds with anti-biofilm effect is, thus, essential. Infections caused by bacterial biofilms are something that nanotechnology has lately shown promise in treating. More and more studies are being conducted to determine whether nanoparticles (NPs) are useful in the fight against bacterial infections. While there have been a small number of clinical trials, there have been several in vitro outcomes examining the effects of antimicrobial NPs. Nanotechnology provides secure delivery platforms for targeted treatments to combat the wide range of microbial infections caused by biofilms. The increase in pharmaceuticals\' bioactive potential is one of the many ways in which nanotechnology has been applied to drug delivery. The current research details the utilization of several nanoparticles in the targeted medication delivery strategy for managing microbial biofilms, including metal and metal oxide nanoparticles, liposomes, micro-, and nanoemulsions, solid lipid nanoparticles, and polymeric nanoparticles. Our understanding of how these nanosystems aid in the fight against biofilms has been expanded through their use.